红癣菌、萌癣菌和吉普森皮真菌生物膜对光动力疗法反应的比较转录组分析。

IF 3.6 3区 生物学 Q2 MYCOLOGY Mycopathologia Pub Date : 2024-06-18 DOI:10.1007/s11046-024-00865-y
Borui Chen, Jinyan Zhang, Juanjuan Li, Yuwen Qian, Binbin Huang, Xiaomo Wu
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引用次数: 0

摘要

皮癣菌生物膜经常导致对标准抗真菌治疗的反应不足和耐药性,从而导致难治性慢性甲癣感染。虽然临床证明抗菌光动力疗法(aPDT)具有显著的抗真菌效果,甚至能够根除皮癣菌生物膜,但人们对 aPDT 的分子机制以及可能拮抗其作用的信号网络的潜在失调却知之甚少。本研究的目的是阐明 aPDT 对抗顽固性甲癣皮癣菌生物膜的分子机制,并破译 aPDT 可能引发的解毒过程,从而促进开发更有效的光动力干预措施。我们应用全基因组比较转录组分析,研究了 aPDT 如何破坏由三种不同的皮癣菌(包括最常见的致病菌红毛癣菌、门冬癣毛癣菌和小孢子菌)形成的甲癣生物膜。我们总共获得了 352.13 Gb 的皮癣菌生物膜转录组纯净数据,其中包括 24.2242 亿个 GC 含量为 51.84% 的读数,分别覆盖了红癣毛癣菌、门冬癣毛癣菌和小孢子菌的 99.9%、98.5% 和 99.4% 的注释基因。全基因组直向同源分析在所有三个物种中发现了 6624 个转录的单拷贝直向同源基因,其中 36.5%、6.8% 和 17.9% 的基因在 aPDT 处理后有差异表达。整合同源分析表明,氧化还原酶活性的上调是所有被研究的皮真菌生物膜对aPDT反应的一种高度保守的解毒信号改变。这项研究为了解 aPDT 抗皮真菌生物膜效应的分子机制提供了新的视角,并成功鉴定了应用 aPDT 后保守的解毒调节机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Comparative Transcriptome Analysis of T. rubrum, T. mentagrophytes, and M. gypseum Dermatophyte Biofilms in Response to Photodynamic Therapy.

Dermatophyte biofilms frequently count for inadequate responses and resistance to standard antifungal treatments, resulting in refractory chronic onychomycosis infection. Although antimicrobial photodynamic therapy (aPDT) has clinically proven to exert significant antifungal effects or even capable of eradicating dermatophyte biofilms, considerably less is known about the molecular mechanisms underlying aPDT and the potential dysregulation of signaling networks that could antagonize its action. The aim of this study is to elucidate the molecular mechanisms underlining aPDT combat against dermatophyte biofilm in recalcitrant onychomycosis and to decipher the potential detoxification processes elicited by aPDT, facilitating the development of more effective photodynamic interventions. We applied genome-wide comparative transcriptome analysis to investigate how aPDT disrupting onychomycosis biofilm formed by three distinct dermatophytes, including Trichophyton rubrum, Trichophyton mentagrophytes, and Microsporum gypseum, the most frequently occurring pathogenic species. In total, 352.13 Gb of clean data were obtained for the transcriptomes of dermatophyte biofilms with or without aPDT treatment, resulting in 2,422.42 million reads with GC content of 51.84%, covering 99.9%, 98.5% and 99.4% of annotated genes of T. rubrum, T. mentagrophytes, and M. gypseum, respectively. The genome-wide orthologous analysis identified 6624 transcribed single-copy orthologous genes in all three species, and 36.5%, 6.8% and 17.9% of which were differentially expressed following aPDT treatment. Integrative orthology analysis demonstrated the upregulation of oxidoreductase activities is a highly conserved detoxification signaling alteration in response to aPDT across all investigated dermatophyte biofilms. This study provided new insights into the molecular mechanisms underneath anti-dermatophyte biofilm effects of aPDT and successfully identified a conserved detoxification regulation upon the aPDT application.

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来源期刊
Mycopathologia
Mycopathologia 生物-真菌学
CiteScore
6.80
自引率
3.60%
发文量
76
审稿时长
3 months
期刊介绍: Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.
期刊最新文献
Dermatophytes: Update on Clinical Epidemiology and Treatment. Invasive Fungal Diseases of Combat Wounds: Burden, Epidemiology, and Mycology. Phaeohyphomycosis Due to Verruconis gallopava: Rare Indolent Pulmonary Infection or Severe Cerebral Fungal Disease? Epidemiological Trends of Mucormycosis in Europe, Comparison with Other Continents. Epidemiological survey of 32,786 culture-positive dermatophytosis cases in Hangzhou from 2018 to 2023.
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