槲皮素共轭 PSC 衍生外泌体通过调节球囊损伤后大鼠颈动脉中的 ERK、Akt 和 NF-κB 信号通路抑制内膜增生。

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-17 DOI:10.1016/j.nano.2024.102763
Xin Mao MD , Yaming Du MD , Rubo Sui MD , Xiaodong Yu MD , Yue Zhu MD , Meiyi Huang MD
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引用次数: 0

摘要

经皮冠状动脉介入治疗血管再狭窄的主要挑战是再狭窄的发生,其定义是新内膜组织的过度增生。在此,我们的研究团队发现,从PSC中获得的外泌体与槲皮素(Q@PSC-E)配伍后,能成功减少Sprague-Dawley大鼠模型中新内膜的增生。此外,还利用紫外可见光、DLS 和傅立叶变换红外光谱表征技术检测了合成的 Q@PSC-E 的物理性质。利用 2-Fr Fogarty 动脉栓塞切除球囊导管对大鼠进行球囊损伤(BI)。通过组织学分析评估了接受 30 mg/kg/d Q@PSC-E 剂量的大鼠组的内膜增生和血管内皮细胞增殖程度。值得注意的是,Q@PSC-E抑制细胞增殖的途径不包括脂氧合酶,[3H]胸苷掺入、MTT和流式细胞术研究均证明了这一点。此外,数据还表明,Q@PSC-E 通过靶向促进细胞生长的特定事件(包括激活 Akt 和 NF-κB、破坏细胞周期进程以及阻碍 ERK 信号通路)来阻碍细胞增殖。
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Quercetin conjugated PSC-derived exosomes to inhibit intimal hyperplasia via modulating the ERK, Akt, and NF-κB signaling pathways in the rat carotid artery post balloon injury

The primary challenge in percutaneous coronary interventions for vascular restenosis is the occurrence of restenosis, which is defined by the excessive proliferation of neointimal tissue. Herein, our research team suggests that exosomes obtained from PSC, when paired with quercetin (Q@PSC-E), successfully reduce neointimal hyperplasia in a Sprague-Dawley rat model. Furthermore, the physical properties of the synthesized Q@PSC-E were examined using UV–vis, DLS, and FT-IR characterization techniques. The rats were subjected to balloon injury (BI) utilizing a 2-Fr Fogarty arterial embolectomy balloon catheter. Intimal hyperplasia and the degree of VSMC proliferation were evaluated using histological analysis in the rat groups that received a dosage of Q@PSC-E at 30 mg/kg/d. Significantly, Q@PSC-E inhibited cell proliferation through a pathway that does not include lipoxygenase, as demonstrated by [3H] thymidine incorporation, MTT, and flow cytometry studies. Additionally, the data indicate that Q@PSC-E hinders cell proliferation by targeting particular events that promote cell growth, including the activation of Akt and NF-κB, disruption of cell-cycle progression and also obstructs the ERK signaling pathway.

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来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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