Cléia Santos Cabral, Alessandro Da Costa Freire Gandara, Fabiana Helena Silva Ribeiro Martins, Elenice Alves Barboza, Maurício Rossato, Ailton Reis
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Fourteen isolates were obtained, and they were initially identified through the morphology of reproductive structures. The isolates were subjected to molecular characterization using phylogeny of the sequences from the ITS region and the Cytochrome Oxidase II gene. Ten isolates of the pathogen were tested for pathogenicity on seedlings of two castor bean accessions and species known to be hosts of <i>P. nicotianae</i>. The isolates were identified as <i>P. nicotianae</i> (12 isolates) and <i>P. palmivora</i> (two isolates). In the pathogenicity test, all isolates were capable of causing disease in castor beans. The isolates of <i>P. nicotianae</i> and <i>P. palmivora</i> varied in pathogenicity to other plant species. This is the first report of <i>P. nicotianae</i> causing collar and root rot in castor bean in the Central-West Region of Brazil. 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引用次数: 0
摘要
蓖麻(Ricinus communis)是巴西一种具有重要经济价值的作物。它被用来生产用于制造化妆品和生物柴油的油。Phytophthora 属包含的病原体寄主植物范围很广,包括观赏植物、水果和蔬菜作物。疫霉菌引起的症状包括领腐病和根腐病。本研究旨在确定在联邦区导致蓖麻秧苗出现领腐和根腐病的疫霉菌种,并测试其致病性。研究人员在巴西联邦区的四个卫星城市采集了有症状的植物样本。获得了 14 个分离株,并通过生殖结构的形态进行了初步鉴定。利用 ITS 区域和细胞色素氧化酶 II 基因序列的系统进化对分离物进行了分子鉴定。对 10 个病原体分离物进行了致病性测试,测试对象是两个蓖麻品种的幼苗,以及已知为烟碱蓖麻菌宿主的物种。经鉴定,这些分离物分别为尼古丁 P.(12 个分离物)和棕榈 P.(2 个分离物)。在致病性试验中,所有分离物都能导致蓖麻发病。P. nicotianae 和 P. palmivora 分离物对其他植物物种的致病性各不相同。这是首次报道 P. nicotianae 在巴西中西部地区引起蓖麻领腐病和根腐病。这也是 P. palmivora 在南美洲引起蓖麻病害的首次报道。
Phytophthora species causing root and crown rot on castor bean (Ricinus communis) in Brazil
Castor bean (Ricinus communis) is a crop of great economic importance in Brazil. It is used for producing oil used in the manufacture of cosmetics and biodiesel. The genus Phytophthora contains pathogens with a wide range of host plants, including ornamentals, fruit and vegetable crops. Among the symptoms induced by Phytophthora species are collar and root rot. This study aimed to identify Phytophthora species causing collar and root rot in castor bean seedlings in the Federal District and test their pathogenicity. Samples of symptomatic plants were collected in four satellite cities in Distrito Federal, Brazil. Fourteen isolates were obtained, and they were initially identified through the morphology of reproductive structures. The isolates were subjected to molecular characterization using phylogeny of the sequences from the ITS region and the Cytochrome Oxidase II gene. Ten isolates of the pathogen were tested for pathogenicity on seedlings of two castor bean accessions and species known to be hosts of P. nicotianae. The isolates were identified as P. nicotianae (12 isolates) and P. palmivora (two isolates). In the pathogenicity test, all isolates were capable of causing disease in castor beans. The isolates of P. nicotianae and P. palmivora varied in pathogenicity to other plant species. This is the first report of P. nicotianae causing collar and root rot in castor bean in the Central-West Region of Brazil. It is also the first report of P. palmivora causing diseases in castor beans in South America.
期刊介绍:
Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays.
Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes.
Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.