Dingfei Ma, Qiangwei Li, Yuanyuan Xie, Youqin Kong, Zhili Ding, Jinyun Ye, Chenglong Wu, Yan Liu
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It also markedly increased the levels of low-density lipoprotein cholesterol, total cholesterol, nonesterified fatty acids, and triglyceride in the liver and serum in a dose-dependent manner. The EA groups had lower values of total superoxide dismutase, total antioxidant capacity, catalase, and higher activities of aspartate aminotransferase and alanine aminotransferase, as dietary EA levels increased. Feeding fish with diets containing EA (20 and 27 g/kg diet) significantly increased the malondialdehyde content. Moreover, dietary EA greatly altered the fatty acid profile in the liver and muscle. It especially elevated the percentages of C18 : 2n-6, C20 : 1n-9, and C22 : 1n-9 while decreasing the C18 : 0 and C16 : 0 levels. When the levels of EA in diets were 12, 20, and 27 g/kg, genes correlated with lipophagy, lipolysis, and <i>β</i>-oxidation were significantly reduced. Meanwhile, genes concerned in triglyceride synthesis were largely increased in the liver and muscle. In summary, high-dose EA (20 g/kg diet) in the diets significantly induced fat accumulation, hepatic oxidative damage, and abnormal lipid metabolism in tilapia. The current findings expand our understanding on the antinutritional role of EA in lipid homeostasis and fish health.</p>\n </div>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6670740","citationCount":"0","resultStr":"{\"title\":\"Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)\",\"authors\":\"Dingfei Ma, Qiangwei Li, Yuanyuan Xie, Youqin Kong, Zhili Ding, Jinyun Ye, Chenglong Wu, Yan Liu\",\"doi\":\"10.1155/2024/6670740\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n <p>Erucic acid (EA) in rapeseed oil has adverse effects on terrestrial animal and fish health. However, its antinutritional role in fish remains unclear due to the limited information on EA. Therefore, this study was conducted to assess the impact of EA on growth performance, antioxidative capacity, fatty acid profile, and lipid metabolism in tilapia. Six diets containing different amounts of EA (0, 3, 6, 12, 20, and 27 g/kg diet) were fed to tilapia (initial weight: 3.01 ± 0.01 g) for 8 weeks. The results exhibited that dietary EA did not affect growth performance but remarkedly increased the crude lipid contents (in the whole body, liver, and muscle). It also markedly increased the levels of low-density lipoprotein cholesterol, total cholesterol, nonesterified fatty acids, and triglyceride in the liver and serum in a dose-dependent manner. The EA groups had lower values of total superoxide dismutase, total antioxidant capacity, catalase, and higher activities of aspartate aminotransferase and alanine aminotransferase, as dietary EA levels increased. Feeding fish with diets containing EA (20 and 27 g/kg diet) significantly increased the malondialdehyde content. Moreover, dietary EA greatly altered the fatty acid profile in the liver and muscle. It especially elevated the percentages of C18 : 2n-6, C20 : 1n-9, and C22 : 1n-9 while decreasing the C18 : 0 and C16 : 0 levels. When the levels of EA in diets were 12, 20, and 27 g/kg, genes correlated with lipophagy, lipolysis, and <i>β</i>-oxidation were significantly reduced. Meanwhile, genes concerned in triglyceride synthesis were largely increased in the liver and muscle. In summary, high-dose EA (20 g/kg diet) in the diets significantly induced fat accumulation, hepatic oxidative damage, and abnormal lipid metabolism in tilapia. 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引用次数: 0
摘要
菜籽油中的芥酸(EA)对陆生动物和鱼类的健康有不利影响。然而,由于有关 EA 的信息有限,其在鱼类中的抗营养作用仍不清楚。因此,本研究评估了 EA 对罗非鱼生长性能、抗氧化能力、脂肪酸组成和脂质代谢的影响。研究人员给罗非鱼(初始体重:3.01 ± 0.01 g)喂食了六种不同含量的 EA 日粮(0、3、6、12、20 和 27 g/kg 日粮),为期 8 周。结果表明,日粮 EA 不影响生长性能,但显著增加了(全身、肝脏和肌肉)粗脂含量。它还显著增加了肝脏和血清中的低密度脂蛋白胆固醇、总胆固醇、非酯化脂肪酸和甘油三酯的含量,且呈剂量依赖性。随着膳食中 EA 含量的增加,EA 组的总超氧化物歧化酶、总抗氧化能力和过氧化氢酶的值降低,天门冬氨酸氨基转移酶和丙氨酸氨基转移酶的活性升高。用含 EA(20 和 27 克/千克日粮)的日粮喂鱼会显著增加丙二醛含量。此外,膳食 EA 还极大地改变了肝脏和肌肉中的脂肪酸谱。尤其是 C18 : 2n-6, C20 :1n-9 和 C22 :1n-9 的百分比,而 C18 :0 和 C16 :0 的水平。当日粮中的 EA 含量为 12、20 和 27 克/千克时,与脂肪吞噬、脂肪分解和 β 氧化相关的基因显著减少。与此同时,肝脏和肌肉中与甘油三酯合成有关的基因大量增加。总之,日粮中的高剂量 EA(20 克/千克日粮)可明显诱导罗非鱼脂肪积累、肝脏氧化损伤和脂质代谢异常。目前的研究结果拓展了我们对 EA 在脂质平衡和鱼类健康中的抗营养作用的认识。
Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)
Erucic acid (EA) in rapeseed oil has adverse effects on terrestrial animal and fish health. However, its antinutritional role in fish remains unclear due to the limited information on EA. Therefore, this study was conducted to assess the impact of EA on growth performance, antioxidative capacity, fatty acid profile, and lipid metabolism in tilapia. Six diets containing different amounts of EA (0, 3, 6, 12, 20, and 27 g/kg diet) were fed to tilapia (initial weight: 3.01 ± 0.01 g) for 8 weeks. The results exhibited that dietary EA did not affect growth performance but remarkedly increased the crude lipid contents (in the whole body, liver, and muscle). It also markedly increased the levels of low-density lipoprotein cholesterol, total cholesterol, nonesterified fatty acids, and triglyceride in the liver and serum in a dose-dependent manner. The EA groups had lower values of total superoxide dismutase, total antioxidant capacity, catalase, and higher activities of aspartate aminotransferase and alanine aminotransferase, as dietary EA levels increased. Feeding fish with diets containing EA (20 and 27 g/kg diet) significantly increased the malondialdehyde content. Moreover, dietary EA greatly altered the fatty acid profile in the liver and muscle. It especially elevated the percentages of C18 : 2n-6, C20 : 1n-9, and C22 : 1n-9 while decreasing the C18 : 0 and C16 : 0 levels. When the levels of EA in diets were 12, 20, and 27 g/kg, genes correlated with lipophagy, lipolysis, and β-oxidation were significantly reduced. Meanwhile, genes concerned in triglyceride synthesis were largely increased in the liver and muscle. In summary, high-dose EA (20 g/kg diet) in the diets significantly induced fat accumulation, hepatic oxidative damage, and abnormal lipid metabolism in tilapia. The current findings expand our understanding on the antinutritional role of EA in lipid homeostasis and fish health.
期刊介绍:
Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers.
Aquaculture Nutrition publishes papers which strive to:
increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research.
improve understanding of the relationships between nutrition and the environmental impact of aquaculture.
increase understanding of the relationships between nutrition and processing, product quality, and the consumer.
help aquaculturalists improve their management and understanding of the complex discipline of nutrition.
help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.