在秀丽隐杆线虫替代模型中对 Achyrocline satureioides 水提取物进行毒理学评估。

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-09-16 Epub Date: 2024-06-21 DOI:10.1080/15287394.2024.2368618
Péterson Alves Santos, Mariana Uczay, Pricila Pflüger, Larissa Aline Carneiro Lobo, Marilise Brittes Rott, Jose Angel Fontenla, Ionara Rodrigues Siqueira, Patrícia Pereira
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引用次数: 0

摘要

Achyrocline satureioides 在巴西俗称为 "marcela",是南美洲的传统药物。A.satureioides的花序可用于治疗多种疾病,包括减轻Sars-Cov-2症状。因此,本研究的目的是利用秀丽隐杆线虫(C. elegans)替代模型,确定 A. satureioides 水提取物(ASAE)的毒性概况。研究进行了生存、繁殖、发育和转代试验。研究了 ASAE 在热应力和氧化剂过氧化氢(H2O2)条件下的作用。此外,还研究了含有高抗氧化酶水平和高daf-16、skn-1和daf-2调控途径品系的秀丽隐杆线虫菌株。发现 ASAE 半数致死浓度值为 77.3 ± 4 毫克/毫升。ASAE 的浓度为 10 毫克/毫升(人类常用浓度),在处理 24 或 72 小时后,L1 或 L4 阶段的蠕虫存活率均未显著降低。ASAE 没有明显改变虫体面积。在 N2 菌株中,ASAE(10 或 25 毫克/毫升)可逆转 H2O2 造成的损害。此外,在含有大量 sod-3、gst-4 和 ctl - 1,2,3 的菌株中,ASAE 保护了 H2O2 造成的损伤,这表明这种植物提取物调节了这些抗氧化系统。即使在极端胁迫下,ASAE 也能激活 daf-16 和 skn-1 的胁迫响应转录途径,而不依赖于 daf-2。数据表明,ASAE 在秀丽隐杆线虫体内的测试浓度显示出可靠的毒性特征,这可能有助于考虑对人类的安全使用。
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Toxicological assessment of the Achyrocline satureioides aqueous extract in the Caenorhabditis elegans alternative model.

Achyrocline satureioides, popularly called "marcela" in Brazil, is used in traditional medicine in South America. A. satureioides, inflorescences are used for many conditions, including to minimize the Sars-Cov-2 symptoms. Therefore, the aim of this study was to determine the toxicity profile of A. satureioides aqueous extract (ASAE), using the Caenorhabditis elegans (C. elegans) alternative model. Survival, reproduction, development, and transgenerational assays were performed. The effects of ASAE were investigated under conditions of thermal stress and presence of oxidant hydrogen peroxide (H2O2). In addition, C. elegans strains containing high antioxidant enzyme levels and elevated lineages of daf-16, skn-1 and daf-2 regulatory pathways were examined. The ASAE LC50 value was found to be 77.3 ± 4 mg/ml. The concentration of ASAE 10 mg/ml (frequently used in humans) did not exhibit a significant reduction in worm survival at either the L1 or L4 stage, after 24 or 72 hr treatment. ASAE did not markedly alter the body area. In N2 strain, ASAE (10 or 25 mg/ml) reversed the damage initiated by H2O2. In addition, ASAE protected the damage produced by H2O2 in strains containing significant levels of sod-3, gst-4 and ctl - 1,2,3, suggesting modulation in these antioxidant systems by this plant extract. ASAE exposure activated daf-16 and skn-1 stress response transcriptional pathways independently of daf-2, even under extreme stress. Data suggest that ASAE, at the concentrations tested in C. elegans, exhibits a reliable toxicity profile, which may contribute to consideration for safe use in humans.

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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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