Tianyu Wang , Lichen Li , Shan Cao , Lele Sun , Gongqi Yu , Qianqian Xia , Tingting Liu , Qing Zhao , Zhenzhen Wang , Chuan Wang , Baoqi Yang , Yongxia Liu , Xuechao Chen , Shengli Chen , Guizhi Zhou , Hong Liu , Yonghu Sun , Furen Zhang
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引用次数: 0
摘要
线性 IgA 大疱性皮肤病(LABD)和疱疹性皮炎(DH)是 IgA 介导的自身免疫性大疱性皮肤病的主要亚型。我们试图通过血清蛋白质组学来了解疾病的病因。我们利用 Olink 高通量蛋白质组学评估了 LAB、DH 和健康对照组的 92 个器官损伤生物标志物。阳性血清蛋白质组学生物标志物与临床特征和 HLA 类型相关。对 IgA 沉积型牛皮癣与对照组进行的靶向蛋白质组学分析表明,生物标志物有所升高。进一步的聚类和富集分析确定了LABD和DH之间不同的聚类,突出了烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶的参与。比较分析发现了区分 LABD 和 DH 的生物标记物,并在皮肤病变中得到了验证。最后,与 DEPs 的定性相关分析表明,六个生物标记物(NBN、NCF2、CAPG、FES、BID 和 PXN)对 DH 患者的预后有较好的影响。这些发现为区分 IgA 沉积性大疱病的疾病亚型提供了潜在的生物标志物。
Targeted serum proteome profiling reveals nicotinamide adenine dinucleotide phosphate (NADPH)-related biomarkers to discriminate linear IgA bullous disorder from dermatitis herpetiformis
Linear IgA bullous dermatosis (LABD) and dermatitis herpetiformis (DH) represent the major subtypes of IgA mediated autoimmune bullous disorders. We sought to understand the disease etiology by using serum proteomics. We assessed 92 organ damage biomarkers in LABD, DH, and healthy controls using the Olink high-throughput proteomics. The positive proteomic serum biomarkers were used to correlate with clinical features and HLA type. Targeted proteomic analysis of IgA deposition bullous disorders vs. controls showed elevated biomarkers. Further clustering and enrichment analyses identified distinct clusters between LABD and DH, highlighting the involvement of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Comparative analysis revealed biomarkers with distinction between LABD and DH and validated in the skin lesion. Finally, qualitative correlation analysis with DEPs suggested six biomarkers (NBN, NCF2, CAPG, FES, BID, and PXN) have better prognosis in DH patients. These findings provide potential biomarkers to differentiate the disease subtype of IgA deposition bullous disease.
期刊介绍:
Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.