同时评估胃癌组织中 HER2 基因扩增、p53 和 Ki67 表达的意义。

IF 2.8 3区 医学 Q2 ONCOLOGY Clinical & Translational Oncology Pub Date : 2025-01-01 Epub Date: 2024-06-22 DOI:10.1007/s12094-024-03534-0
Su-Nan Wang, Yang-Kun Wang, Chao-Ya Zhu, Bo Jiang, Dong-Feng Ge, Ying-Ying Li
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引用次数: 0

摘要

研究目的本研究的主要目的是探讨同时评估胃癌组织中 HER2 基因扩增及 p53 和 Ki67 表达的意义:方法:采用荧光原位杂交(FISH)和免疫组化(IHC)方法检测78例胃癌患者的HER2基因扩增情况以及HER2、p53和Ki67蛋白的表达水平:HER2蛋白的表达率为43.6%(34/78),其中17.9%(14/78)为HER2蛋白3+,14.1%(11/78)为HER2蛋白2+,11.5%(9/78)为HER2蛋白1+。利用 FISH 技术,HER2 基因扩增率为 19.2%(15/78),其中包括 3 例 HER2 基因簇扩增、5 例大颗粒扩增、4 例点状扩增和 3 例高度多体。胃癌细胞中p53的阳性率为52.6%(41/78),其中62.8%(49/78)的患者ki67增殖指数≤30,37.2%(29/78)的患者ki67增殖指数大于30。胃癌组织中 HER2 基因、p53 和 ki67 的表达率与胃癌分期和淋巴结转移均有显著相关性(PHER2 基因扩增率和基因拷贝数与 p53 和 ki67 的表达率呈正相关。将这些评估结合起来,可为评估胃肿瘤细胞的转移潜力、疾病进展和预后提供重要见解。这对指导制定个体化治疗策略至关重要。
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Significance of concurrent evaluation of HER2 gene amplification and p53 and Ki67 expression in gastric cancer tissues.

Objective: The primary objective of this study is to explore the significance of concurrent evaluation of HER2 gene amplification and p53 and Ki67 expression in gastric cancer tissues.

Methods: Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) methodologies were used to detect HER2 gene amplification, as well as the expression levels of HER2, p53, and Ki67 proteins, across a group of 78 gastric cancer cases.

Results: The expression rate of the HER2 protein was determined to be 43.6% (34/78), with 17.9% (14/78) categorized as HER2 protein 3 + , 14.1% (11/78) as HER2 protein 2 + , and 11.5% (9/78) as HER2 protein 1 + . Using FISH technology, the HER2 gene amplification rate was identified as 19.2% (15/78), including 3 cases of HER2 gene cluster amplification, 5 cases of large granular amplification, 4 cases of punctate amplification, and 3 cases of high polysomy. The positive rate of p53 in gastric cancer cells was 52.6% (41/78), with 62.8% (49/78) of patients exhibiting a ki67 proliferation index ≤ 30, and 37.2% (29/78) accounting for a ki67 proliferation index > 30. The expression rates of the HER2 gene, p53, and ki67 in gastric cancer tissues were significantly associated with both gastric cancer staging and lymph node metastasis (P < 0.05).

Conclusion: The HER2 gene amplification rate and gene copy number exhibit a positive correlation with the expression rates of p53 and ki67. Combining these assessments can provide crucial insights into the assessment of metastatic potential, disease progression, and prognosis of gastric tumor cells. This holds paramount importance in steering the formulation of individualized treatment strategies.

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来源期刊
CiteScore
6.20
自引率
2.90%
发文量
240
审稿时长
1 months
期刊介绍: Clinical and Translational Oncology is an international journal devoted to fostering interaction between experimental and clinical oncology. It covers all aspects of research on cancer, from the more basic discoveries dealing with both cell and molecular biology of tumour cells, to the most advanced clinical assays of conventional and new drugs. In addition, the journal has a strong commitment to facilitating the transfer of knowledge from the basic laboratory to the clinical practice, with the publication of educational series devoted to closing the gap between molecular and clinical oncologists. Molecular biology of tumours, identification of new targets for cancer therapy, and new technologies for research and treatment of cancer are the major themes covered by the educational series. Full research articles on a broad spectrum of subjects, including the molecular and cellular bases of disease, aetiology, pathophysiology, pathology, epidemiology, clinical features, and the diagnosis, prognosis and treatment of cancer, will be considered for publication.
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