Vladimir Farkaš, Anđelo Beletić, Josipa Kuleš, Funmilola Clara Thomas, Dina Rešetar Maslov, Ivana Rubić, Miroslav Benić, Goran Bačić, Nino Mačešić, Ines Jović, Vida Eraghi, Andrea Gelemanović, David Eckersall, Vladimir Mrljak
{"title":"亚临床牛乳腺炎的生物标志物:基于高通量 TMT 的蛋白质组学研究。","authors":"Vladimir Farkaš, Anđelo Beletić, Josipa Kuleš, Funmilola Clara Thomas, Dina Rešetar Maslov, Ivana Rubić, Miroslav Benić, Goran Bačić, Nino Mačešić, Ines Jović, Vida Eraghi, Andrea Gelemanović, David Eckersall, Vladimir Mrljak","doi":"10.1007/s11259-024-10442-9","DOIUrl":null,"url":null,"abstract":"<p><p>Mastitis represents the biggest threat to the health and productivity of dairy cows, leading to substantial economic losses in milk production. It manifests in two forms: clinical mastitis, easily diagnosed by visible symptoms, and subclinical mastitis (SCM), which lacks overt clinical signs. SCM's elusive nature often results in it going undetected, thus facilitating the spread of the disease-causing agent due to lack of treatment. Finding a reliable biomarker for early SCM would reduce the possibility of mastitis spreading in the herd, reduce the need for antibiotic use and ultimately reduce milk losses for producers. Utilizing state-of-the-art proteomics techniques, 138 milk samples from dairy cows in continental Croatia underwent analysis. These samples were categorized into four groups based on the Zagreb Mastitis Test (ZMT) and microbiological analysis: lowSCC- (n = 20), lowSCC + (n = 20), medSCC + (n = 79), and highSCC + (n = 19). A total of 386 proteins were identified and quantified, with 76 proteins showing significant differential abundances among the groups. Many of these proteins are linked to the innate immune system, as well as neutrophil and platelet degranulation processes. Through fold changes observed between groups, 15 proteins exhibiting biomarker characteristics for subclinical mastitis (SCM) were identified. Among these, five proteins-cathelicidins (-1, -4, and -7), lactoferrin, and haptoglobin-showed particular promise.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biomarkers for subclinical bovine mastitis: a high throughput TMT-based proteomic investigation.\",\"authors\":\"Vladimir Farkaš, Anđelo Beletić, Josipa Kuleš, Funmilola Clara Thomas, Dina Rešetar Maslov, Ivana Rubić, Miroslav Benić, Goran Bačić, Nino Mačešić, Ines Jović, Vida Eraghi, Andrea Gelemanović, David Eckersall, Vladimir Mrljak\",\"doi\":\"10.1007/s11259-024-10442-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mastitis represents the biggest threat to the health and productivity of dairy cows, leading to substantial economic losses in milk production. 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Biomarkers for subclinical bovine mastitis: a high throughput TMT-based proteomic investigation.
Mastitis represents the biggest threat to the health and productivity of dairy cows, leading to substantial economic losses in milk production. It manifests in two forms: clinical mastitis, easily diagnosed by visible symptoms, and subclinical mastitis (SCM), which lacks overt clinical signs. SCM's elusive nature often results in it going undetected, thus facilitating the spread of the disease-causing agent due to lack of treatment. Finding a reliable biomarker for early SCM would reduce the possibility of mastitis spreading in the herd, reduce the need for antibiotic use and ultimately reduce milk losses for producers. Utilizing state-of-the-art proteomics techniques, 138 milk samples from dairy cows in continental Croatia underwent analysis. These samples were categorized into four groups based on the Zagreb Mastitis Test (ZMT) and microbiological analysis: lowSCC- (n = 20), lowSCC + (n = 20), medSCC + (n = 79), and highSCC + (n = 19). A total of 386 proteins were identified and quantified, with 76 proteins showing significant differential abundances among the groups. Many of these proteins are linked to the innate immune system, as well as neutrophil and platelet degranulation processes. Through fold changes observed between groups, 15 proteins exhibiting biomarker characteristics for subclinical mastitis (SCM) were identified. Among these, five proteins-cathelicidins (-1, -4, and -7), lactoferrin, and haptoglobin-showed particular promise.
期刊介绍:
Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial.
The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.