胆盐对金头鲷肠上皮功能的影响

IF 2.5 3区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Fish Physiology and Biochemistry Pub Date : 2024-08-01 Epub Date: 2024-06-25 DOI:10.1007/s10695-024-01369-8
J Fuentes, S F Gregório, F Fonseca, R Robles-Arozarena, J A Martos-Sitcha, F J Moyano
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引用次数: 0

摘要

在现代水产养殖的背景下,为了减少鱼类饮食对鱼粉/海洋配料的依赖,人们开始探索以植物为基础的蛋白质来源作为潜在的替代品,这种饮食转变会破坏鱼类的胆汁酸谱。因此,人们正在寻找胆汁盐作为添加剂。然而,人为提高肠道胆汁酸水平可能会严重影响粘膜功能。因此,我们在这里利用上皮电压钳技术,在乌星腔室中探索了(i) 醋去氧胆酸(CDC)、(ii) 由两种胆汁酸(3% 胆酸和 97% 去氧胆酸)形成的混合物(MIX)和(iii) 共轭胆盐牛磺胆酸钠(TC)在金头鲷(Sparus aurata)肠道中的调节作用。我们在 50-500 μg/ml 的浓度范围内对胆盐进行了测试,所有胆盐都促进了离子的吸收。然而,在后肠观察到了明显的浓度依赖性和更明显的离子转运效应。另一方面,胆盐对组织阻力没有影响或影响较小。然而,有迹象表明,高浓度(500 微克/毫升)的 MIX 可能会产生不利影响,无论在哪个肠道区域,使用 FITC-葡聚糖(4 kD)测量的组织通透性都会增加三倍,从而表明高浓度胆盐会改变肠道通透性。我们的研究结果强调了在考虑将某种胆盐用作膳食补充剂时考虑肠道功能的重要性。看来,胆盐无论是单独作用还是联合作用,都会通过影响上皮离子转运功能在协调营养吸收方面发挥关键作用。然而,要完全掌握胆盐对离子转运的影响因区域而异的细微差别,以及在水产养殖中对营养吸收的最终影响,还需要进一步的研究。
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Effect of bile salts on intestinal epithelial function in gilthead seabream (Sparus aurata).

In the context of modern aquaculture, the effort to reduce the reliance on fishmeal/marine ingredients in fish diets has led to the exploration of plant-based protein sources as potential substitutes, a dietary shift that disrupts the bile acid profile in fish. Therefore, bile salts are being sought as additives. However, artificially increased intestinal levels of bile acids may significantly impact mucosal function. Therefore, here, we explored the regulatory role in the intestine of gilthead sea bream (Sparus aurata) of (i) chenodeoxycholic acid (CDC), (ii) a mixture formed by two bile acids, 3% cholic acid and 97% deoxycholic acid (MIX), and (iii) a conjugated bile salt sodium taurocholate (TC) in Ussing chambers with the epithelial voltage clamp technique. We tested the bile salts in a 50-500 μg/ml concentration range, and all of them promoted ion absorption. Yet, clear concentration-dependent and more pronounced effects on the ion transport were observed in the posterior intestine. On the other hand, bile salts had no or minor effects on tissue resistance. However, there are indications that the MIX could have adverse effects at high concentrations (500 μg/ml), promoting a threefold increase in tissue permeability measured using FITC-dextran (4 kD) regardless of the intestinal region, thus suggesting an alteration in intestinal permeability at high bile salt concentrations. The findings from our study emphasize the importance of considering intestinal function when contemplating the possible use of a particular bile salt as a dietary supplement. It appears that bile salts, whether acting individually or in combination, play a pivotal role in orchestrating nutrient absorption by influencing the function of epithelial ion transport. However further research is needed to fully grasp the region-dependent nuances of bile salt effects on ion transport and the ultimate consequences for nutrient absorption in the context of fish aquaculture.

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来源期刊
Fish Physiology and Biochemistry
Fish Physiology and Biochemistry 农林科学-生化与分子生物学
CiteScore
5.60
自引率
6.90%
发文量
106
审稿时长
4 months
期刊介绍: Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.
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