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引用次数: 0
摘要
在特定器官或细胞中发挥作用的纳米装置是合成生物学的终极目标之一。DNA 纳米技术(如 DNA 折纸)的最新进展使我们能够构建纳米装置,将有效载荷(如药物)输送到肿瘤。然而,由于 DNA 纳米结构的脆弱性和较低的靶向能力,向特定器官递送仍然很困难。在这里,我们构建了坚韧的DNA折纸,使我们能够在苛刻的条件(低pH值)下将DNA折纸封装到脂基纳米颗粒(LNPs)中,利用器官特异性递送感兴趣的基因(GOI)。我们发现,通过低温电子显微镜(Cryo-EM)显示的不同 LNP 结构的贡献,DNA 折纸封装的 LNPs 可以提高有效载荷 GOIs(mRNA 和 siRNA)在小鼠器官内的功能。这些数据应成为未来利用DNA折纸纳米器件控制器官特异性基因表达的基础。
Organ-Specific Gene Expression Control Using DNA Origami-Based Nanodevices
Nanodevices that function in specific organs or cells are one of the ultimate goals of synthetic biology. The recent progress in DNA nanotechnology such as DNA origami has allowed us to construct nanodevices to deliver a payload (e.g., drug) to the tumor. However, delivery to specific organs remains difficult due to the fragility of the DNA nanostructure and the low targeting capability of the DNA nanostructure. Here, we constructed tough DNA origami that allowed us to encapsulate the DNA origami into lipid-based nanoparticles (LNPs) under harsh conditions (low pH), harnessing organ-specific delivery of the gene of interest (GOI). We found that DNA origami-encapsulated LNPs can increase the functionality of payload GOIs (mRNA and siRNA) inside mouse organs through the contribution from different LNP structures revealed by cryogenic electron microscope (Cryo-EM). These data should be the basis for future organ-specific gene expression control using DNA origami nanodevices.
期刊介绍:
Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including:
- Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale
- Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies
- Modeling and simulation of synthetic, assembly, and interaction processes
- Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance
- Applications of nanoscale materials in living and environmental systems
Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.