microRNA-8514-5p调控促脂肪激素/冠状病毒相关肽受体，从而影响木虱的发育和繁殖。

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-06-26 DOI:10.1002/ps.8267

Senbo Pang, Shuo Wang, Muhammad Asad, Jiajing Yu, Guifang Lin, Jinzhi Chen, Cuiying Sun, Pengrong Huang, Yanpeng Chang, Hui Wei, Guang Yang

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引用次数: 0

摘要

背景Dicer1 在调节昆虫的发育和繁殖过程中起着至关重要的作用。Dicer1基因敲除会导致木虱（Plutella xylostella）蛹畸形、低卵黄率和低繁殖力，但这一现象背后的机制尚不清楚。本研究旨在确定Dicer1基因敲除株（ΔPxDcr-1）中不同表达的基因和miRNA，并评估它们对木虱繁殖和发育的影响：结果：Dicer1基因敲除影响了包括促脂肪激素/可拉宗素相关肽受体（PxACPR）在内的基因表达。在ΔPxDcr-1中，PxACPR的表达上调，miR-8514-5p的表达下调。双荧光素酶报告实验和牵引实验表明，miR-8514-5p 在体外和体内都与 PxACPR 结合。表达谱显示，在野生型菌株的不同发育阶段，PxACPR mRNA 与 miR-8514-5p 呈负相关。向蛹前期注射miR-8514-5p agomir和ACPR双链RNA（dsPxACPR）都抑制了PxACPR的mRNA水平，导致蛹死亡率和畸形率高、繁殖力和孵化率低，这与ΔPxDcr-1的表型一致。注射 miR-8514-5p antagomir 与注射 miR-8514-5p agomir 的表型相似。此外，注射 miR-8514-5p antagomir 还能显著缓解 dsPxACPR 引起的表型：这些结果表明，miR-8514-5p 通过调控 PxACPR 影响木虱的发育和繁殖，而 PxACPR 的表达平衡对木虱的发育和繁殖至关重要。© 2024 化学工业学会。

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microRNA-8514-5p regulates adipokinetic hormone/corazonin-related peptide receptor to affect development and reproduction of Plutella xylostella.

Background: Dicer1 plays a crucial role in regulating the development and reproduction of insects. Knockout of Dicer1 causes pupal deformity, low eclosion and low fecundity in Plutella xylostella, but the mechanism behind this phenomenon is not clear. This study aims to identify differentially-expressed genes and miRNAs in the Dicer1-knockout strain (ΔPxDcr-1) and assess their impact on the reproduction and development of P. xylostella.

Results: The knockout of Dicer1 affected the expression of genes including the adipokinetic hormone/corazonin-related peptide receptor (PxACPR). The expression of PxACPR was upregulated, and the expression of miR-8514-5p was downregulated in ΔPxDcr-1 of P. xylostella. The dual luciferase reporter assay and pull-down assay showed that miR-8514-5p bound to PxACPR in vitro and in vivo. The expression profiles demonstrated a negative correlation between PxACPR mRNA and miR-8514-5p in different developmental stages of the wild-type strain. Both the miR-8514-5p agomir and double-stranded RNA of ACPR (dsPxACPR) injected into the pre-pupae inhibited the mRNA level of PxACPR, causing high mortality and deformity of pupae, and low fecundity and hatching rate, which were consistent with the phenotype of ΔPxDcr-1. The injection of miR-8514-5p antagomir caused a similar phenotype to the injection of miR-8514-5p agomir. Additionally, the injection of miR-8514-5p antagomir significantly rescued the phenotype caused by dsPxACPR.

Conclusion: These results indicate that miR-8514-5p affects the development and reproduction of P. xylostella by regulating PxACPR, and the homeostasis of PxACPR expression is essential for the development and reproduction of P. xylostella. © 2024 Society of Chemical Industry.

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.