使用浊度计进行实时三重环介导等温扩增（LAMP），检测虾传染性皮下和造血坏死病毒（IHHNV）。

IF 1.5 4区 农林科学 Q3 FISHERIES Journal of aquatic animal health Pub Date : 2024-06-24 DOI:10.1002/aah.10218
Narong Arunrut, Sarocha Jitrakorn, Benyatip Tondee, Vanvimon Saksmerprome, Wansika Kiatpathomchai
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引用次数: 0

摘要

目的：世界动物卫生组织（World Organization for Animal Health）仍在监管对虾传染性皮下和造血坏死病毒（IHHNV）。现有的疾病鉴定方法费时费力，需要昂贵的设备，还需要专业知识，因此限制了养殖场对虾病筛查的可及性。环路介导等温扩增法（LAMP）因其能够高灵敏度和特异性地检测抑制性物质而得到认可：方法：我们开发了一种实时三重 LAMP 检测方法，它结合了护理点检测的简便性和浊度计的准确性。我们的技术使用一组三种 LAMP 引物，可在 45 分钟内通过单次反应快速扩增 DNA，且检测限低（10 个拷贝/次反应）：结果：我们检测了 192 份不同来源的虾样本，证明了我们方法的临床实用性，在检测 IHHNV DNA 方面达到了 100% 的特异性（95% 置信区间 = 93.40-100.00%）、100% 的灵敏度（97.36-100.00%）和 100% 的准确度（98.10-100.00%），与标准的定量聚合酶链反应检测相比，科恩卡帕值（1）较高：结论：我们的方法具有很高的技术就绪水平，使其成为任何实时 LAMP 检测的通用平台，而且成本低、操作简单，非常适合在对虾养殖中快速部署和使用。
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Real-time triplex loop-mediated isothermal amplification (LAMP) using a turbidimeter for detection of shrimp infectious hypodermal and hematopoietic necrosis virus (IHHNV)

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来源期刊
Journal of aquatic animal health
Journal of aquatic animal health 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
25
审稿时长
>24 weeks
期刊介绍： The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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