Zhengyi Zhang, Yulong Guo, Mingsheng Zhuang, Fugang Liu, Zhongyan Xia, Zhihao Zhang, Fan Yang, Huayan Zeng, Yueguo Wu, Jiaxing Huang, Kai Xu, Jilian Li
{"title":"中华大黄蜂肠道微生物组多样性与生物地理学","authors":"Zhengyi Zhang, Yulong Guo, Mingsheng Zhuang, Fugang Liu, Zhongyan Xia, Zhihao Zhang, Fan Yang, Huayan Zeng, Yueguo Wu, Jiaxing Huang, Kai Xu, Jilian Li","doi":"10.1128/msystems.00459-24","DOIUrl":null,"url":null,"abstract":"<p><p>Gut microbiota of the bumblebee is critical as it modulates the health and fitness of the host. However, the mechanisms underlying the formation and maintenance of the diversity of bumblebee gut bacteria over a long period of evolution have yet to be elucidated. In particular, the gut bacterial diversity and community assembly processes of <i>Bombus pyrosoma</i> across the Chinese border remain unclear. In this study, we systematically carried out unprecedented sampling of 513 workers of the species <i>Bombus pyrosoma</i> across the Chinese landscape and used full-length 16S rRNA gene sequencing to examine their gut microbiota diversity and biogeography. The gut microbiota composition and community structure of <i>Bombus pyrosoma</i> from different geographical locations were diverse. On the whole, the gut bacteria <i>Gilliamella</i> and <i>Snodgrassella</i> are dominant in bumblebees, but opportunistic pathogens <i>Serratia</i> and <i>Pseudomonas</i> are dominant in some sampling sites such as Hb15, Gs1, Gs45, Qhs15, and Ssx35. All or part of environmental factors such as latitude, annual mean temperature, elevation, human footprint, population density, and annual precipitation can affect the alpha diversity and community structure of gut bacteria. Further analysis showed that the assembly and shift of bumblebee gut bacterial communities under geographical variation were mainly driven by the stochastic drift of the neutral process rather than by variable selection of niche differentiation. In conclusion, our unprecedented sampling uncovers bumblebee gut microbiome diversity and shifts over evolutionary time.</p><p><strong>Importance: </strong>The microbiotas associated with organisms facilitates host health and fitness, and the homeostasis status of gut microbiota also reflects the habitat security faced by the host. In addition, managing gut microbiota is important to improve bumblebee health by understanding the ecological process of the gut microbiome. Thus, we first carried out an runprecedented sampling of 513 workers of the species <i>Bombus pyrosoma</i> across the Chinese landscape and used full-length 16S rRNA gene sequencing to uncover their gut microbiota diversity and biogeography. Our study provides new insights into the understanding of gut microbiome diversity and shifts for Chinese Bumblebee over evolutionary time.</p>","PeriodicalId":18819,"journal":{"name":"mSystems","volume":null,"pages":null},"PeriodicalIF":5.0000,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264632/pdf/","citationCount":"0","resultStr":"{\"title\":\"Gut microbiome diversity and biogeography for Chinese bumblebee <i>Bombus pyrosoma</i>.\",\"authors\":\"Zhengyi Zhang, Yulong Guo, Mingsheng Zhuang, Fugang Liu, Zhongyan Xia, Zhihao Zhang, Fan Yang, Huayan Zeng, Yueguo Wu, Jiaxing Huang, Kai Xu, Jilian Li\",\"doi\":\"10.1128/msystems.00459-24\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Gut microbiota of the bumblebee is critical as it modulates the health and fitness of the host. However, the mechanisms underlying the formation and maintenance of the diversity of bumblebee gut bacteria over a long period of evolution have yet to be elucidated. In particular, the gut bacterial diversity and community assembly processes of <i>Bombus pyrosoma</i> across the Chinese border remain unclear. In this study, we systematically carried out unprecedented sampling of 513 workers of the species <i>Bombus pyrosoma</i> across the Chinese landscape and used full-length 16S rRNA gene sequencing to examine their gut microbiota diversity and biogeography. The gut microbiota composition and community structure of <i>Bombus pyrosoma</i> from different geographical locations were diverse. On the whole, the gut bacteria <i>Gilliamella</i> and <i>Snodgrassella</i> are dominant in bumblebees, but opportunistic pathogens <i>Serratia</i> and <i>Pseudomonas</i> are dominant in some sampling sites such as Hb15, Gs1, Gs45, Qhs15, and Ssx35. All or part of environmental factors such as latitude, annual mean temperature, elevation, human footprint, population density, and annual precipitation can affect the alpha diversity and community structure of gut bacteria. Further analysis showed that the assembly and shift of bumblebee gut bacterial communities under geographical variation were mainly driven by the stochastic drift of the neutral process rather than by variable selection of niche differentiation. In conclusion, our unprecedented sampling uncovers bumblebee gut microbiome diversity and shifts over evolutionary time.</p><p><strong>Importance: </strong>The microbiotas associated with organisms facilitates host health and fitness, and the homeostasis status of gut microbiota also reflects the habitat security faced by the host. In addition, managing gut microbiota is important to improve bumblebee health by understanding the ecological process of the gut microbiome. 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Gut microbiome diversity and biogeography for Chinese bumblebee Bombus pyrosoma.
Gut microbiota of the bumblebee is critical as it modulates the health and fitness of the host. However, the mechanisms underlying the formation and maintenance of the diversity of bumblebee gut bacteria over a long period of evolution have yet to be elucidated. In particular, the gut bacterial diversity and community assembly processes of Bombus pyrosoma across the Chinese border remain unclear. In this study, we systematically carried out unprecedented sampling of 513 workers of the species Bombus pyrosoma across the Chinese landscape and used full-length 16S rRNA gene sequencing to examine their gut microbiota diversity and biogeography. The gut microbiota composition and community structure of Bombus pyrosoma from different geographical locations were diverse. On the whole, the gut bacteria Gilliamella and Snodgrassella are dominant in bumblebees, but opportunistic pathogens Serratia and Pseudomonas are dominant in some sampling sites such as Hb15, Gs1, Gs45, Qhs15, and Ssx35. All or part of environmental factors such as latitude, annual mean temperature, elevation, human footprint, population density, and annual precipitation can affect the alpha diversity and community structure of gut bacteria. Further analysis showed that the assembly and shift of bumblebee gut bacterial communities under geographical variation were mainly driven by the stochastic drift of the neutral process rather than by variable selection of niche differentiation. In conclusion, our unprecedented sampling uncovers bumblebee gut microbiome diversity and shifts over evolutionary time.
Importance: The microbiotas associated with organisms facilitates host health and fitness, and the homeostasis status of gut microbiota also reflects the habitat security faced by the host. In addition, managing gut microbiota is important to improve bumblebee health by understanding the ecological process of the gut microbiome. Thus, we first carried out an runprecedented sampling of 513 workers of the species Bombus pyrosoma across the Chinese landscape and used full-length 16S rRNA gene sequencing to uncover their gut microbiota diversity and biogeography. Our study provides new insights into the understanding of gut microbiome diversity and shifts for Chinese Bumblebee over evolutionary time.
mSystemsBiochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
10.50
自引率
3.10%
发文量
308
审稿时长
13 weeks
期刊介绍:
mSystems™ will publish preeminent work that stems from applying technologies for high-throughput analyses to achieve insights into the metabolic and regulatory systems at the scale of both the single cell and microbial communities. The scope of mSystems™ encompasses all important biological and biochemical findings drawn from analyses of large data sets, as well as new computational approaches for deriving these insights. mSystems™ will welcome submissions from researchers who focus on the microbiome, genomics, metagenomics, transcriptomics, metabolomics, proteomics, glycomics, bioinformatics, and computational microbiology. mSystems™ will provide streamlined decisions, while carrying on ASM''s tradition of rigorous peer review.