Ling Lu, Dan Guo, Le-Min Hong, Yu-Wen Jiang, Hong-Ming Fan, Chun-Qun Huang, Jin-Feng Lu, Jie Chen, Hong-Hui Xu, Hong-Ming Huang
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The correlation of <i>LINC00475</i> with patients' characteristics was analyzed.</p><p><strong>Results: </strong>Compared with control groups, the expression of <i>LINC00475</i> was up-regulated in serum of MM patients and MM cell lines (all <i>P</i> < 0.05). ROC curve analysis showed that the optimal cut-off value of <i>LINC00475</i> was 262.4, the area under curve (AUC) was 0.924(95%<i>CI</i> : 0.884-0.964), and sensitivity and specificity was 83.3% and 91.7%, respectively, which indicated that <i>LINC00475</i> had good evaluation value in MM patients. Compared with low-<i>LINC00475</i> expression group, patients in high-<i>LINC00475</i> expression group had higher levels of β<sub>2</sub>microglobulin (β<sub>2</sub>-MG) and Cystatin C (Cys-C) but lower albumin (ALB) (all <i>P</i> < 0.05). Compared with MM patients with International Staging System (ISS) stage I, the expression level of <i>LINC00475</i> was significantly higher in patients with stage II and III (both <i>P</i> < 0.05).</p><p><strong>Conclusion: </strong><i>LINC00475</i> is helpful to distinguish MM patients from healthy adults, which is correlated with the prognostic indicators such as β<sub>2</sub>-MG, ALB, and ISS stage.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Expression and Clinical Significance of <i>LINC00475</i> in Multiple Myeloma].\",\"authors\":\"Ling Lu, Dan Guo, Le-Min Hong, Yu-Wen Jiang, Hong-Ming Fan, Chun-Qun Huang, Jin-Feng Lu, Jie Chen, Hong-Hui Xu, Hong-Ming Huang\",\"doi\":\"10.19746/j.cnki.issn.1009-2137.2024.03.020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the relative expression level and clinical significance of <i>LINC00475</i> in serum of patients with multiple myeloma (MM).</p><p><strong>Methods: </strong>The expression of <i>LINC00475</i> in serum of 108 MM patients and five MM cell lines including RPMI 8226, NCI-H929, U266, OPM2 and CAG were detected by real-time fluorescence quantitative PCR. The diagnostic value of <i>LINC00475</i> in MM was evaluated by receiver operating characteristic (ROC) curve analysis. The correlation of <i>LINC00475</i> with patients' characteristics was analyzed.</p><p><strong>Results: </strong>Compared with control groups, the expression of <i>LINC00475</i> was up-regulated in serum of MM patients and MM cell lines (all <i>P</i> < 0.05). ROC curve analysis showed that the optimal cut-off value of <i>LINC00475</i> was 262.4, the area under curve (AUC) was 0.924(95%<i>CI</i> : 0.884-0.964), and sensitivity and specificity was 83.3% and 91.7%, respectively, which indicated that <i>LINC00475</i> had good evaluation value in MM patients. Compared with low-<i>LINC00475</i> expression group, patients in high-<i>LINC00475</i> expression group had higher levels of β<sub>2</sub>microglobulin (β<sub>2</sub>-MG) and Cystatin C (Cys-C) but lower albumin (ALB) (all <i>P</i> < 0.05). Compared with MM patients with International Staging System (ISS) stage I, the expression level of <i>LINC00475</i> was significantly higher in patients with stage II and III (both <i>P</i> < 0.05).</p><p><strong>Conclusion: </strong><i>LINC00475</i> is helpful to distinguish MM patients from healthy adults, which is correlated with the prognostic indicators such as β<sub>2</sub>-MG, ALB, and ISS stage.</p>\",\"PeriodicalId\":35777,\"journal\":{\"name\":\"中国实验血液学杂志\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国实验血液学杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.03.020\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国实验血液学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.03.020","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
目的研究多发性骨髓瘤(MM)患者血清中LINC00475的相对表达水平及其临床意义:方法:采用实时荧光定量 PCR 法检测 108 例 MM 患者及 RPMI 8226、NCI-H929、U266、OPM2 和 CAG 五种 MM 细胞系血清中 LINC00475 的表达。接收者操作特征曲线(ROC)分析评估了LINC00475在MM中的诊断价值。分析了LINC00475与患者特征的相关性:结果:与对照组相比,LINC00475在MM患者和MM细胞系血清中的表达呈上调趋势(均P < 0.05)。ROC曲线分析显示,LINC00475的最佳临界值为262.4,曲线下面积(AUC)为0.924(95%CI:0.884-0.964),灵敏度和特异度分别为83.3%和91.7%,这表明LINC00475在MM患者中具有良好的评估价值。与低LINC00475表达组相比,高LINC00475表达组患者的β2微球蛋白(β2-MG)和胱抑素C(Cys-C)水平较高,但白蛋白(ALB)水平较低(均P<0.05)。与国际分期系统(ISS)Ⅰ期的MM患者相比,Ⅱ期和Ⅲ期患者的LINC00475表达水平明显更高(均为P<0.05):结论:LINC00475有助于区分MM患者和健康成人,它与β2-MG、ALB和ISS分期等预后指标相关。
[Expression and Clinical Significance of LINC00475 in Multiple Myeloma].
Objective: To investigate the relative expression level and clinical significance of LINC00475 in serum of patients with multiple myeloma (MM).
Methods: The expression of LINC00475 in serum of 108 MM patients and five MM cell lines including RPMI 8226, NCI-H929, U266, OPM2 and CAG were detected by real-time fluorescence quantitative PCR. The diagnostic value of LINC00475 in MM was evaluated by receiver operating characteristic (ROC) curve analysis. The correlation of LINC00475 with patients' characteristics was analyzed.
Results: Compared with control groups, the expression of LINC00475 was up-regulated in serum of MM patients and MM cell lines (all P < 0.05). ROC curve analysis showed that the optimal cut-off value of LINC00475 was 262.4, the area under curve (AUC) was 0.924(95%CI : 0.884-0.964), and sensitivity and specificity was 83.3% and 91.7%, respectively, which indicated that LINC00475 had good evaluation value in MM patients. Compared with low-LINC00475 expression group, patients in high-LINC00475 expression group had higher levels of β2microglobulin (β2-MG) and Cystatin C (Cys-C) but lower albumin (ALB) (all P < 0.05). Compared with MM patients with International Staging System (ISS) stage I, the expression level of LINC00475 was significantly higher in patients with stage II and III (both P < 0.05).
Conclusion: LINC00475 is helpful to distinguish MM patients from healthy adults, which is correlated with the prognostic indicators such as β2-MG, ALB, and ISS stage.