Fan Yang, Ling Ling, Ya-Yin Hou, Lan Yang, Mei Sun, Fang-Fang Wang, Duo-Nan Yu
{"title":"[不同类型贫血症中 MiR-144/451 的表达]","authors":"Fan Yang, Ling Ling, Ya-Yin Hou, Lan Yang, Mei Sun, Fang-Fang Wang, Duo-Nan Yu","doi":"10.19746/j.cnki.issn.1009-2137.2024.03.026","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression level and clinical correlation of <i>microRNA-144/451</i> gene cluster (<i>miR-144/451</i>) in different types of anemia.</p><p><strong>Methods: </strong>The peripheral blood of patients with aplastic anemia (AA), myelodysplastic syndrome (MDS) and diffuse large B-cell lymphoma (DLBCL) who had been diagnosed with anemia for the first time and after chemotherapy were collected. The expression levels of <i>miR-144</i> and <i>miR-451</i> were measured by RT-qPCR, and the correlation between the expression levels of <i>miR-144</i> and <i>miR-451</i> and routine laboratory indexes was analyzed by Spearman correlation analysis.</p><p><strong>Results: </strong>The expression levels of <i>miR-144</i> and <i>miR-451</i> in the peripheral blood of AA and MDS patients were significantly lower than those in normal controls (all <i>P</i> < 0.01). No statistical differences were observed in the expression level of <i>miR-144</i> in three subgroups of DLBCL patients (<i>P</i> >0.05), while the expression level of <i>miR-451</i> in peripheral blood of three subgroups of DLBCL patients were significantly higher than those in normal controls (all <i>P</i> < 0.05). Correlation analysis showed that the expression levels of <i>miR-144</i> and <i>miR-451</i> in AA patients were positively correlated with red blood cell distribution width-coefficient of variation (RDW-CV) (<i>r</i> =0.629, 0.574). There were no significant correlations between the expression levels of <i>miR-144</i> and <i>miR-451</i> and laboratory parameters in MDS and DLBCL patients.</p><p><strong>Conclusion: </strong>Different types of anemia disorders have varying levels of <i>miR-144</i> and <i>miR-451</i> expression, which is anticipated to develop into a secondary diagnostic and differential diagnostic indicator for clinical anemia diseases.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Expression of <i>MiR-144/451</i> in Different Types of Anemia].\",\"authors\":\"Fan Yang, Ling Ling, Ya-Yin Hou, Lan Yang, Mei Sun, Fang-Fang Wang, Duo-Nan Yu\",\"doi\":\"10.19746/j.cnki.issn.1009-2137.2024.03.026\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the expression level and clinical correlation of <i>microRNA-144/451</i> gene cluster (<i>miR-144/451</i>) in different types of anemia.</p><p><strong>Methods: </strong>The peripheral blood of patients with aplastic anemia (AA), myelodysplastic syndrome (MDS) and diffuse large B-cell lymphoma (DLBCL) who had been diagnosed with anemia for the first time and after chemotherapy were collected. The expression levels of <i>miR-144</i> and <i>miR-451</i> were measured by RT-qPCR, and the correlation between the expression levels of <i>miR-144</i> and <i>miR-451</i> and routine laboratory indexes was analyzed by Spearman correlation analysis.</p><p><strong>Results: </strong>The expression levels of <i>miR-144</i> and <i>miR-451</i> in the peripheral blood of AA and MDS patients were significantly lower than those in normal controls (all <i>P</i> < 0.01). No statistical differences were observed in the expression level of <i>miR-144</i> in three subgroups of DLBCL patients (<i>P</i> >0.05), while the expression level of <i>miR-451</i> in peripheral blood of three subgroups of DLBCL patients were significantly higher than those in normal controls (all <i>P</i> < 0.05). Correlation analysis showed that the expression levels of <i>miR-144</i> and <i>miR-451</i> in AA patients were positively correlated with red blood cell distribution width-coefficient of variation (RDW-CV) (<i>r</i> =0.629, 0.574). There were no significant correlations between the expression levels of <i>miR-144</i> and <i>miR-451</i> and laboratory parameters in MDS and DLBCL patients.</p><p><strong>Conclusion: </strong>Different types of anemia disorders have varying levels of <i>miR-144</i> and <i>miR-451</i> expression, which is anticipated to develop into a secondary diagnostic and differential diagnostic indicator for clinical anemia diseases.</p>\",\"PeriodicalId\":35777,\"journal\":{\"name\":\"中国实验血液学杂志\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国实验血液学杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.03.026\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国实验血液学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2024.03.026","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
目的研究microRNA-144/451基因簇(miR-144/451)在不同类型贫血症中的表达水平及临床相关性:方法:收集首次确诊贫血和化疗后的再生障碍性贫血(AA)、骨髓增生异常综合征(MDS)和弥漫大 B 细胞淋巴瘤(DLBCL)患者的外周血。采用RT-qPCR法测定miR-144和miR-451的表达水平,并采用Spearman相关分析法分析miR-144和miR-451的表达水平与常规实验室指标的相关性:结果:miR-144和miR-451在AA和MDS患者外周血中的表达水平明显低于正常对照组(P均<0.01)。miR-144 在三个亚组 DLBCL 患者外周血中的表达水平无统计学差异(P >0.05),而 miR-451 在三个亚组 DLBCL 患者外周血中的表达水平明显高于正常对照组(均 P <0.05)。相关分析显示,AA 患者 miR-144 和 miR-451 的表达水平与红细胞分布宽度-变异系数(RDW-CV)呈正相关(r =0.629,0.574)。MDS和DLBCL患者中miR-144和miR-451的表达水平与实验室指标无明显相关性:结论:不同类型的贫血症有不同水平的miR-144和miR-451表达,有望发展成为临床贫血症的辅助诊断和鉴别诊断指标。
[Expression of MiR-144/451 in Different Types of Anemia].
Objective: To investigate the expression level and clinical correlation of microRNA-144/451 gene cluster (miR-144/451) in different types of anemia.
Methods: The peripheral blood of patients with aplastic anemia (AA), myelodysplastic syndrome (MDS) and diffuse large B-cell lymphoma (DLBCL) who had been diagnosed with anemia for the first time and after chemotherapy were collected. The expression levels of miR-144 and miR-451 were measured by RT-qPCR, and the correlation between the expression levels of miR-144 and miR-451 and routine laboratory indexes was analyzed by Spearman correlation analysis.
Results: The expression levels of miR-144 and miR-451 in the peripheral blood of AA and MDS patients were significantly lower than those in normal controls (all P < 0.01). No statistical differences were observed in the expression level of miR-144 in three subgroups of DLBCL patients (P >0.05), while the expression level of miR-451 in peripheral blood of three subgroups of DLBCL patients were significantly higher than those in normal controls (all P < 0.05). Correlation analysis showed that the expression levels of miR-144 and miR-451 in AA patients were positively correlated with red blood cell distribution width-coefficient of variation (RDW-CV) (r =0.629, 0.574). There were no significant correlations between the expression levels of miR-144 and miR-451 and laboratory parameters in MDS and DLBCL patients.
Conclusion: Different types of anemia disorders have varying levels of miR-144 and miR-451 expression, which is anticipated to develop into a secondary diagnostic and differential diagnostic indicator for clinical anemia diseases.