通过质控细胞仪确定子宫肌瘤的免疫格局

{"title":"通过质控细胞仪确定子宫肌瘤的免疫格局","authors":"","doi":"10.1016/j.xfss.2024.06.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>To study the differences in immune cell<span> profiles in uterine fibroids<span> (Fibs) and matched myometrium (Myo).</span></span></p></div><div><h3>Design</h3><p>Observational study.</p></div><div><h3>Setting</h3><p>Laboratory study.</p></div><div><h3>Patient(s)</h3><p><span>The study included tissue that was collected from 10 pairs of Fib and matched Myo from women, not on hormonal medications, undergoing hysterectomy and </span>myomectomy.</p></div><div><h3>Intervention(s)</h3><p>None.</p></div><div><h3>Main Outcome Measure(s)</h3><p>Differences in immune cell and cytokine composition between Fib and matched Myo.</p></div><div><h3>Result(s)</h3><p>The mass cytometry<span><span><span><span> analysis indicated that Fibs had a significantly higher number of natural killer (NK) cells, total macrophages, M2 macrophages<span>, and conventional dendritic cells when compared with matched Myo from the same patient. In contrast, Fibs had significantly fewer CD3<span> and CD4 T cells when compared with Myo. The mass </span></span></span>cytometry analysis results did not show any significant difference in the number of resting mast cells. Immunoflurorescent and immunohistochemical imaging confirmed the cytometry by time of flight results, showing a significantly higher number of NK cells, tryptase-positive mast cells indicative of mast </span>cell activation, total macrophages, and M2 cells in Fibs and a significantly lower number of CD3 and CD4 T cells. The cytokine assay revealed significantly increased levels of </span>human interferon<span> α2, interleukin (IL)-1α, and platelet-derived growth factor AA and significantly lower levels of macrophage colony-stimulating factor and IL-1 receptor antagonist in Fib.</span></span></p></div><div><h3>Conclusion(s)</h3><p>Our results show significant differences in immune cell populations and cytokine levels between Fib and Myo. These differences could account for the increased inflammation in fib and a potential mechanism by which these tumors evade the immune system. These findings provide a foundation for further studies exploring the role of immune cells in Fib development.</p></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 3","pages":"Pages 272-282"},"PeriodicalIF":0.0000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The immune landscape of uterine fibroids as determined by mass cytometry\",\"authors\":\"\",\"doi\":\"10.1016/j.xfss.2024.06.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>To study the differences in immune cell<span> profiles in uterine fibroids<span> (Fibs) and matched myometrium (Myo).</span></span></p></div><div><h3>Design</h3><p>Observational study.</p></div><div><h3>Setting</h3><p>Laboratory study.</p></div><div><h3>Patient(s)</h3><p><span>The study included tissue that was collected from 10 pairs of Fib and matched Myo from women, not on hormonal medications, undergoing hysterectomy and </span>myomectomy.</p></div><div><h3>Intervention(s)</h3><p>None.</p></div><div><h3>Main Outcome Measure(s)</h3><p>Differences in immune cell and cytokine composition between Fib and matched Myo.</p></div><div><h3>Result(s)</h3><p>The mass cytometry<span><span><span><span> analysis indicated that Fibs had a significantly higher number of natural killer (NK) cells, total macrophages, M2 macrophages<span>, and conventional dendritic cells when compared with matched Myo from the same patient. In contrast, Fibs had significantly fewer CD3<span> and CD4 T cells when compared with Myo. The mass </span></span></span>cytometry analysis results did not show any significant difference in the number of resting mast cells. Immunoflurorescent and immunohistochemical imaging confirmed the cytometry by time of flight results, showing a significantly higher number of NK cells, tryptase-positive mast cells indicative of mast </span>cell activation, total macrophages, and M2 cells in Fibs and a significantly lower number of CD3 and CD4 T cells. The cytokine assay revealed significantly increased levels of </span>human interferon<span> α2, interleukin (IL)-1α, and platelet-derived growth factor AA and significantly lower levels of macrophage colony-stimulating factor and IL-1 receptor antagonist in Fib.</span></span></p></div><div><h3>Conclusion(s)</h3><p>Our results show significant differences in immune cell populations and cytokine levels between Fib and Myo. These differences could account for the increased inflammation in fib and a potential mechanism by which these tumors evade the immune system. These findings provide a foundation for further studies exploring the role of immune cells in Fib development.</p></div>\",\"PeriodicalId\":73012,\"journal\":{\"name\":\"F&S science\",\"volume\":\"5 3\",\"pages\":\"Pages 272-282\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"F&S science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666335X24000338\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"F&S science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666335X24000338","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的:研究子宫肌瘤(Fib)和匹配子宫肌层(Myo)免疫细胞谱的差异:研究子宫肌瘤(Fib)和匹配子宫肌瘤(Myo)免疫细胞谱的差异:观察性研究研究对象: 实验室研究研究包括从接受子宫切除术和子宫肌瘤剔除术的未服用激素药物的妇女的10对子宫肌瘤和匹配子宫肌层中收集的组织:主要结果测量:结果:质控细胞仪分析表明,与来自同一患者的匹配子宫肌瘤相比,子宫肌瘤的 NK 细胞、巨噬细胞总数、M2 巨噬细胞和传统树突状细胞数量明显较高。相比之下,子宫肌瘤的 CD3 和 CD4 T 细胞数量明显少于子宫肌层。质谱分析显示,静止肥大细胞的数量没有明显差异。IFC 和 IHC 成像证实了 CytoF 的结果,显示子宫肌瘤中的 CD3 和 CD4 T 细胞数量明显较高:我们的研究结果表明,Fib 和 Myo 的免疫细胞群和细胞因子水平存在明显差异。在 Fib 中,巨噬细胞、M2 巨噬细胞、NK 细胞和树突状细胞的总数明显增加,而 CD3 和 CD4 T 细胞则明显减少。IHC 证实静息肥大细胞总数无差异,但纤维中胰蛋白酶阳性肥大细胞明显增加。与匹配的子宫肌层相比,纤维的IFNA2、IL-1α和PDGF-AA水平明显更高,而IL-1RA和M-CSF水平明显更低。这些发现为进一步研究免疫细胞在纤维发育中的作用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The immune landscape of uterine fibroids as determined by mass cytometry

Objective

To study the differences in immune cell profiles in uterine fibroids (Fibs) and matched myometrium (Myo).

Design

Observational study.

Setting

Laboratory study.

Patient(s)

The study included tissue that was collected from 10 pairs of Fib and matched Myo from women, not on hormonal medications, undergoing hysterectomy and myomectomy.

Intervention(s)

None.

Main Outcome Measure(s)

Differences in immune cell and cytokine composition between Fib and matched Myo.

Result(s)

The mass cytometry analysis indicated that Fibs had a significantly higher number of natural killer (NK) cells, total macrophages, M2 macrophages, and conventional dendritic cells when compared with matched Myo from the same patient. In contrast, Fibs had significantly fewer CD3 and CD4 T cells when compared with Myo. The mass cytometry analysis results did not show any significant difference in the number of resting mast cells. Immunoflurorescent and immunohistochemical imaging confirmed the cytometry by time of flight results, showing a significantly higher number of NK cells, tryptase-positive mast cells indicative of mast cell activation, total macrophages, and M2 cells in Fibs and a significantly lower number of CD3 and CD4 T cells. The cytokine assay revealed significantly increased levels of human interferon α2, interleukin (IL)-1α, and platelet-derived growth factor AA and significantly lower levels of macrophage colony-stimulating factor and IL-1 receptor antagonist in Fib.

Conclusion(s)

Our results show significant differences in immune cell populations and cytokine levels between Fib and Myo. These differences could account for the increased inflammation in fib and a potential mechanism by which these tumors evade the immune system. These findings provide a foundation for further studies exploring the role of immune cells in Fib development.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
F&S science
F&S science Endocrinology, Diabetes and Metabolism, Obstetrics, Gynecology and Women's Health, Urology
CiteScore
2.00
自引率
0.00%
发文量
0
审稿时长
51 days
期刊最新文献
Mitochondrial activator BGP-15 protects sperm quality against oxidative damage and improves embryo developmental competence. Receptive window might be shorter in patients with endometriosis and lesions cyclically prepare for implantation. Sphingosine 1-phosphate acts as proliferative and fibrotic cue in leiomyoma cells. Blood-based inflammatory markers in female infertility: evidence from Mendelian randomization analysis. Phthalates are detected in the follicular fluid of adolescents and oocyte donors with associated changes in the cumulus cell transcriptome.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1