{"title":"用微型仪器消除背景荧光,对牛奶中的四环素进行灵敏、高通量的时间分辨发光检测","authors":"Jiahui Luo, and , Zece Zhu*, ","doi":"10.1021/acs.analchem.4c01508","DOIUrl":null,"url":null,"abstract":"<p >Fluorescence detection has always suffered from high background fluorescence from real samples such as milk. Therefore, cumbersome pretreatments of samples were necessary to remove the fluorescent substances but led to long processing times and low efficiency. Time-resolved luminescence detection is a powerful technique for eliminating short-lived background fluorescence without additional pretreatments. However, the related instruments are usually equipped with high-speed excitation sources and detectors, which are always bulky and expensive. Herein, we developed a low-cost and miniaturized imaging system for high-throughput time-gated luminescence detection. An UV LED array was used to excite multiple samples, the luminescence of which could be detected by a smartphone simultaneously. An analog circuit was designed to synchronize the LED to the mechanical chopper to eliminate the background signals resulting from scattering and short-lived autofluorescence. Compared to other synchronous circuits based on FPGAs and microcontrollers, this analog circuit required no programming and memory. For the first time, high-throughput time-resolved luminescence detection of tetracycline in milk without any separation or enrichment was achieved by utilizing a smartphone as a camera, and the scattered signals and the background fluorescence were eliminated efficiently. The limit of detection reached as low as 53 nM (∼0.024 ppm), lower than the residue limit set by the European Union. 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引用次数: 0
摘要
荧光检测一直受到来自牛奶等真实样品的高背景荧光的影响。因此,必须对样品进行繁琐的预处理,以去除荧光物质,但这导致处理时间长、效率低。时间分辨荧光检测是一种无需额外预处理即可消除短寿命背景荧光的强大技术。然而,相关仪器通常都配备有高速激发光源和检测器,体积庞大且价格昂贵。在此,我们开发了一种用于高通量时间门控荧光检测的低成本微型成像系统。紫外 LED 阵列用于激发多个样品,智能手机可同时检测这些样品的发光。设计了一个模拟电路,使 LED 与机械斩波器同步,以消除散射和短寿命自发荧光产生的背景信号。与其他基于 FPGA 和微控制器的同步电路相比,这种模拟电路无需编程和内存。利用智能手机作为摄像头,首次实现了牛奶中四环素的高通量时间分辨发光检测,无需任何分离或富集,并有效消除了散射信号和背景荧光。检测限低至 53 nM(∼0.024 ppm),低于欧盟规定的残留限量。这种高通量时间门控荧光检测方法可用于对许多具有高背景荧光的真实样品进行定量分析。
Sensitive and High-Throughput Time-Resolved Luminescence Detection of Tetracycline in Milk for Eliminating Background Fluorescence on a Miniaturized Apparatus
Fluorescence detection has always suffered from high background fluorescence from real samples such as milk. Therefore, cumbersome pretreatments of samples were necessary to remove the fluorescent substances but led to long processing times and low efficiency. Time-resolved luminescence detection is a powerful technique for eliminating short-lived background fluorescence without additional pretreatments. However, the related instruments are usually equipped with high-speed excitation sources and detectors, which are always bulky and expensive. Herein, we developed a low-cost and miniaturized imaging system for high-throughput time-gated luminescence detection. An UV LED array was used to excite multiple samples, the luminescence of which could be detected by a smartphone simultaneously. An analog circuit was designed to synchronize the LED to the mechanical chopper to eliminate the background signals resulting from scattering and short-lived autofluorescence. Compared to other synchronous circuits based on FPGAs and microcontrollers, this analog circuit required no programming and memory. For the first time, high-throughput time-resolved luminescence detection of tetracycline in milk without any separation or enrichment was achieved by utilizing a smartphone as a camera, and the scattered signals and the background fluorescence were eliminated efficiently. The limit of detection reached as low as 53 nM (∼0.024 ppm), lower than the residue limit set by the European Union. This high-throughput time-gated luminescence detection method can be used for quantitative analysis of many real samples with high background fluorescence.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.