多柔比星处理的人冠状动脉内皮细胞的细胞因子反应：体外实验结果。

Q3 Biochemistry, Genetics and Molecular Biology Biomeditsinskaya khimiya Pub Date : 2024-06-01 DOI:10.18097/PBMC20247003156

A V Sinitskaya, E A Velikanova, E A Senokosova, M Yu Sinitsky, M V Khutornaya, M A Asanov, A O Poddubnyak, A V Ponasenko

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引用次数: 0

摘要

使用酶联免疫吸附试验和 qPCR 评估了在多柔比星（2 μg/ml 和 6 μg/ml）存在下培养的原发性冠状动脉内皮细胞的细胞因子谱。在这些浓度的多柔比星存在下培养细胞 24 小时后，下列基因的表达上调：IL6（分别增加 2.30 倍和 2.66 倍）、IL1B（分别增加 1.25 倍和 3.44 倍）、CXCL8（分别增加 6.47 倍和 6.42 倍）、MIF（分别增加 2.34 倍和 2.28 倍）、CCL2（分别增加 4.22 倍和 3.98 倍）。在这些条件下，下列基因被下调：IL10、IL1R2、TNF。在多柔比星（2 μg/ml 和 6 μg/ml）存在下培养细胞 24 小时也会增加 IL-6 的分泌。

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The cytokine response of human coronary artery endothelial cells treated with doxorubicin: results of an in vitro experiment.

The cytokine profile of primary coronary artery endothelial cells cultivated in the presence of doxorubicin (2 μg/ml and 6 μg/ml) was evaluated using enzyme-linked immunosorbent assay and qPCR. Cultivation of cells in the presence of these concentrations of doxorubicin for 24 h, upregulated expression of the following genes: IL6 (by 2.30 and 2.66 times, respectively), IL1B (by 1.25 and 3.44 times), and CXCL8 (by 6.47 times and 6.42 times), MIF (2.34 and 2.28 times), CCL2 (4.22 and 3.98 times). Under these conditions the following genes were downregulated: IL10, IL1R2, TNF. Cultivation of cells in the presence of doxorubicin (2 μg/ml and 6 μg/ml) for 24 h also increased the secretion of IL-6.

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来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).