Qiong Wang, Junzhao Duan, Jian Hong, Kexin Ding, Fumin Tai, Jie Zhu, Hanjiang Fu, Xiaofei Zheng, Changhui Ge
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Thus, this study aimed to elucidate the mechanisms underlying IR-induced intestinal injury and the protective effects of CBLB502 against this condition in mice.</p><p><strong>Materials and methods: </strong>Mice were administered 0.2 mg/kg CBLB502 before IR at different doses for different time points, and then the survival rate, body weight, hemogram, and histopathology of the mice were analyzed.</p><p><strong>Results: </strong>CBLB502 reduced IR-induced intestinal injury. RNA-seq analysis revealed that different doses and durations of IR induced different regulatory patterns. CBLB502 protected against intestinal injury mainly after IR by reversing the expression of IR-induced genes and regulating immune processes and metabolic pathways.</p><p><strong>Conclusion: </strong>This study preliminarily describes the regulatory mechanism of IR-induced intestinal injury and the potential molecular protective mechanism of CBLB502, providing a basis for identifying the functional genes and molecular mechanisms that mediate protection against IR-induced injury.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11215590/pdf/","citationCount":"0","resultStr":"{\"title\":\"Toll-like Receptor Agonist CBLB502 Protects Against Radiation-induced Intestinal Injury in Mice.\",\"authors\":\"Qiong Wang, Junzhao Duan, Jian Hong, Kexin Ding, Fumin Tai, Jie Zhu, Hanjiang Fu, Xiaofei Zheng, Changhui Ge\",\"doi\":\"10.21873/invivo.13613\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background/aim: </strong>The small intestine is one of the organs most vulnerable to ionizing radiation (IR) damage. 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引用次数: 0
摘要
背景/目的:小肠是最容易受到电离辐射(IR)损伤的器官之一。然而,防止 IR 引起的肠道损伤的方法却很有限。CBLB502是一种来自沙门氏菌鞭毛蛋白的Toll样受体5(TLR5)激动剂,可对多种组织和器官产生辐射保护作用。然而,CBLB502 保护红外诱导的肠道损伤的分子机制仍不清楚。因此,本研究旨在阐明红外诱导小鼠肠道损伤的机制以及 CBLB502 对这种情况的保护作用:结果:CBLB502可减少IR诱导的肠道损伤:结果:CBLB502减轻了IR引起的肠道损伤。RNA-seq分析显示,不同剂量和持续时间的红外诱导了不同的调控模式。CBLB502主要通过逆转IR诱导的基因表达、调节免疫过程和代谢途径来保护IR后的肠道损伤:本研究初步描述了IR诱导肠道损伤的调控机制和CBLB502潜在的分子保护机制,为确定IR诱导损伤的功能基因和分子机制提供了依据。
Toll-like Receptor Agonist CBLB502 Protects Against Radiation-induced Intestinal Injury in Mice.
Background/aim: The small intestine is one of the organs most vulnerable to ionizing radiation (IR) damage. However, methods to protect against IR-induced intestinal injury are limited. CBLB502, a Toll-like receptor 5 (TLR5) agonist from Salmonella flagellin, exerts radioprotective effects on various tissues and organs. However, the molecular mechanisms by which CBLB502 protects against IR-induced intestinal injury remain unclear. Thus, this study aimed to elucidate the mechanisms underlying IR-induced intestinal injury and the protective effects of CBLB502 against this condition in mice.
Materials and methods: Mice were administered 0.2 mg/kg CBLB502 before IR at different doses for different time points, and then the survival rate, body weight, hemogram, and histopathology of the mice were analyzed.
Results: CBLB502 reduced IR-induced intestinal injury. RNA-seq analysis revealed that different doses and durations of IR induced different regulatory patterns. CBLB502 protected against intestinal injury mainly after IR by reversing the expression of IR-induced genes and regulating immune processes and metabolic pathways.
Conclusion: This study preliminarily describes the regulatory mechanism of IR-induced intestinal injury and the potential molecular protective mechanism of CBLB502, providing a basis for identifying the functional genes and molecular mechanisms that mediate protection against IR-induced injury.
期刊介绍:
IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management.
The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.