掺氮芦丁结合葡萄糖碳点对阿尔茨海默病的治疗潜力

IF 2.5 3区 工程技术 Q2 BIOLOGY Yale Journal of Biology and Medicine Pub Date : 2024-06-28 eCollection Date: 2024-06-01 DOI:10.59249/EWOI2166
Sana Khan, Faria Hasan Jatala, Alveena Muti, Noor Afza, Aneeqa Noor, Sara Mumtaz, Saima Zafar
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引用次数: 0

摘要

血脑屏障(BBB)阻碍了许多治疗神经系统疾病药物的使用。最近,掺氮碳点(NCDs)作为一种有希望穿越血脑屏障的纳米载体出现了。我们研究的主要重点是评估 NCDs 在对症治疗阿尔茨海默病(AD)方面的有效性。在这项研究中,我们开发了与芦丁结合的 NCDs 并对其进行了表征。尽管芦丁有很多益处,但通过 NCDs 转运芦丁用于治疗老年痴呆症的研究以前还没有过。我们使用傅立叶变换红外光谱和紫外可见光谱对颗粒进行了表征,然后使用原子力显微镜进行了观察。设计优化和验证完成后,我们通过溶血试验进行了体内测试,以优化剂量。我们在 AlCl3 诱导的大鼠注意力缺失模型中进行了初步体外测试,腹腔注射单剂量 10 mg/kg NCDs-芦丁。有趣的是,单剂量 10 毫克/千克 NCDs-芦丁产生的行为效果与腹腔注射 50 毫克/千克芦丁 1 个月产生的效果相同。同样,组织学和生物标志物(SOD2 和 TLR4)也显示,NCDs-芦丁对神经元损失、炎症和氧化应激具有显著的保护作用。因此,NCDs-芦丁是治疗神经系统疾病的一种很有前景的方法。
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Therapeutic Potential of Nitrogen-Doped Rutin-Bound Glucose Carbon Dots for Alzheimer's Disease.

The blood-brain barrier (BBB) prevents the use of many drugs for the treatment of neurological disorders. Recently, nitrogen-doped carbon dots (NCDs) have emerged as promising nanocarriers to cross BBB. The primary focus of our study was to evaluate the effectiveness of NCDs for the symptomatic treatment of Alzheimer's disease (AD). In this study, we developed and characterized NCDs bound to rutin, a flavonoid with known benefits for AD. Despite its benefits, the transportation of rutin via NCDs for AD therapy has not been explored previously. We characterized the particles using FTIR and UV-visible spectroscopy followed by atomic force microscopy. Once the design was optimized and validated, we performed in vivo testing via a hemolytic assay to optimize the dosage. Preliminary in vitro testing was performed in AlCl3-induced rat models of AD whereby a single dose of 10 mg/kg NCDs-rutin was administered intraperitoneally. Interestingly, this single dose of 10 mg/kg NCDs-rutin produced the same behavioral effects as 50 mg/kg rutin administered intraperitoneally for 1 month. Similarly, histological and biomarker profiles (SOD2 and TLR4) also presented significant protective effects of NCDs-rutin against neuronal loss, inflammation, and oxidative stress. Hence, NCDs-rutin are a promising approach for the treatment of neurological diseases.

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来源期刊
Yale Journal of Biology and Medicine
Yale Journal of Biology and Medicine Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology
CiteScore
5.00
自引率
0.00%
发文量
41
期刊介绍: The Yale Journal of Biology and Medicine (YJBM) is a graduate and medical student-run, peer-reviewed, open-access journal dedicated to the publication of original research articles, scientific reviews, articles on medical history, personal perspectives on medicine, policy analyses, case reports, and symposia related to biomedical matters. YJBM is published quarterly and aims to publish articles of interest to both physicians and scientists. YJBM is and has been an internationally distributed journal with a long history of landmark articles. Our contributors feature a notable list of philosophers, statesmen, scientists, and physicians, including Ernst Cassirer, Harvey Cushing, Rene Dubos, Edward Kennedy, Donald Seldin, and Jack Strominger. Our Editorial Board consists of students and faculty members from Yale School of Medicine and Yale University Graduate School of Arts & Sciences. All manuscripts submitted to YJBM are first evaluated on the basis of scientific quality, originality, appropriateness, contribution to the field, and style. Suitable manuscripts are then subject to rigorous, fair, and rapid peer review.
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