用魔鬼爪预处理对局灶性脑缺血大鼠运动活动、梗塞体积和神经元密度的影响

IF 1.9 Q3 CHEMISTRY, MEDICINAL Avicenna Journal of Phytomedicine Pub Date : 2024-07-01 DOI:10.22038/AJP.2024.24294
Shima Shirzad, Mona Riyahi Rad, Mohammad Rezaei, Mitra Tayaranian Marvian, Arman Abroumand Gholami, Fatemeh Forouzanfar, Mansoureh Sabzalizadeh, Hamed Ghazavi, Farzaneh Vafaee
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引用次数: 0

摘要

目的:中风是一种发病率很高的破坏性疾病,影响着全球数百万人。恶魔爪(DCW)是一种非洲本地植物,其抗炎、抗氧化和神经保护特性已得到研究。我们推测,DCW 可以保护脑缺血引起的脑损伤:大鼠随机分为四组。假组和对照组(Ctrl)使用蒸馏水载体进行预处理。DCW预处理剂量分别为200和400 mg/kg。采用丝状或血管内闭塞法进行大脑中动脉闭塞(MCAO)。采用三苯基氯化四氮唑(TTC)染色法检测梗死区和半影体积。通过苏木精染色法测量DCW的神经保护作用。通过神经功能缺损评分、转体表现和开放场地测试评估运动能力:结果:TTC 染色显示,与对照组相比,DCW/400 组能维持半影的结构并缩小梗死体积(p 结论:该研究表明,DCW/400 预处理能维持半影的结构并缩小梗死体积:该研究表明,使用具有神经元保护潜能的 DCW 预处理可减少 MCAO 后的梗死面积并恢复运动功能。
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Effect of pretreatment with Devil's Claw on locomotor activity, infarct volume, and neuronal density in focal cerebral ischemia in rats.

Objective: Stroke is a highly prevalent and devastating condition affecting millions worldwide. The Devil's Claw (DCW) plant is a native African plant whose anti-inflammatory, antioxidant, and neuroprotective properties have been investigated. We postulated that DCW could protect the brain injury caused by cerebral ischemia.

Materials and methods: The rats were randomly divided into four groups. The sham and control (Ctrl) groups received pretreatment with a distilled water vehicle. Doses of 200 and 400 mg/kg were selected for pretreatment with DCW. The filament or intravascular occlusion method was used for middle cerebral artery occlusion (MCAO). The Triphenyl tetrazolium chloride (TTC) staining method was used to investigate the infarct zone and penumbra volume. The neuroprotective effect of DCW was measured by hematoxylin staining. Movement performance was evaluated from neurological deficit score, rotarod performance, and open field tests.

Results: TTC staining showed that the DCW/400 group could maintain the penumbra's structure and reduce the infarct volume compared to the Ctrl group (p<0.001). Histological studies confirmed the neuroprotective properties of DCW at doses of 200 and 400 mg/kg compared to the Ctrl group (p<0.01 and p<0.0001, respectively). The results of behavioral tests showed an improvement in behavioral performance in pretreatment 400 mg/kg doses compare to Ctrl group (p<0.0001).

Conclusion: The study showed that pretreatment with DCW with its neuron protection potential reduces the infarct area and restores motor function after MCAO.

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来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
期刊最新文献
Aloe vera supplementation improves cardiovascular risk factors in hemodialysis patients: A randomized, double-blind, placebo-controlled trial. An in vitro antiviral evaluation of punicalagin toward influenza A virus. Apigenin attenuates serum concentrations of TNF-a, interleukin 1b and interleukin 6 in lipopolysaccharide-stimulated rats. Comparison of effects of P-coumaric acid and coumarin on colorectal cancer cell line by inducing apoptosis and autophagy. Effect of pretreatment with Devil's Claw on locomotor activity, infarct volume, and neuronal density in focal cerebral ischemia in rats.
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