{"title":"用于高灵敏度和选择性测定生物液体中谷胱甘肽及细胞成像的多功能掺杂 Eu 的碳点纳米探针","authors":"Haitham Saad Al-mashriqi , Pascaline Sanga , Jia Chen , Eskandar Qaed , Jing Xiao , Xin Li , Hongdeng Qiu","doi":"10.1016/j.carbon.2024.119380","DOIUrl":null,"url":null,"abstract":"<div><p>Glutathione (GSH) holds a vital role in biological systems, serving diverse cellular functions. Selective determination of GSH over cysteine (Cys) and homocysteine (Hcy) stays challenging due to the shared functional groups among these biothiols. In this study, a versatile Eu-doped carbon dots (Eu-CDs) nanoprobe has been developed for the exceptionally sensitive and selective detection of GSH in biological fluids. The synthesized Eu-CDs exhibited high solubility in water, stability to salt and pH variations, and consistent fluorescence emission, boasting a quantum yield of 40.67 %. Following the optimization of detection parameters, a robust linear relationship for GSH was established in the concentration range of 0.0–50 μM, with a detection limit of 0.03 μM. The viability of the technique in actual samples was evaluated by successfully measuring the GSH in urine and human serum samples with a range recovery from 90.6 % to 98.5 %. Furthermore, Eu-CDs proved to be exceptionally suitable for imaging Hela cells owing to their low cytotoxicity and remarkable biocompatibility. As a result, the developed fluorescent biosensor demonstrated significant potential for widespread application in clinical diagnostic analyses.</p></div>","PeriodicalId":262,"journal":{"name":"Carbon","volume":null,"pages":null},"PeriodicalIF":10.5000,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multifunctional Eu-doped carbon dots nanoprobe for highly sensitive and selective determination of glutathione in biological fluid and cell imaging\",\"authors\":\"Haitham Saad Al-mashriqi , Pascaline Sanga , Jia Chen , Eskandar Qaed , Jing Xiao , Xin Li , Hongdeng Qiu\",\"doi\":\"10.1016/j.carbon.2024.119380\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Glutathione (GSH) holds a vital role in biological systems, serving diverse cellular functions. Selective determination of GSH over cysteine (Cys) and homocysteine (Hcy) stays challenging due to the shared functional groups among these biothiols. In this study, a versatile Eu-doped carbon dots (Eu-CDs) nanoprobe has been developed for the exceptionally sensitive and selective detection of GSH in biological fluids. The synthesized Eu-CDs exhibited high solubility in water, stability to salt and pH variations, and consistent fluorescence emission, boasting a quantum yield of 40.67 %. Following the optimization of detection parameters, a robust linear relationship for GSH was established in the concentration range of 0.0–50 μM, with a detection limit of 0.03 μM. The viability of the technique in actual samples was evaluated by successfully measuring the GSH in urine and human serum samples with a range recovery from 90.6 % to 98.5 %. Furthermore, Eu-CDs proved to be exceptionally suitable for imaging Hela cells owing to their low cytotoxicity and remarkable biocompatibility. As a result, the developed fluorescent biosensor demonstrated significant potential for widespread application in clinical diagnostic analyses.</p></div>\",\"PeriodicalId\":262,\"journal\":{\"name\":\"Carbon\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":10.5000,\"publicationDate\":\"2024-06-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Carbon\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0008622324005992\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbon","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0008622324005992","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Multifunctional Eu-doped carbon dots nanoprobe for highly sensitive and selective determination of glutathione in biological fluid and cell imaging
Glutathione (GSH) holds a vital role in biological systems, serving diverse cellular functions. Selective determination of GSH over cysteine (Cys) and homocysteine (Hcy) stays challenging due to the shared functional groups among these biothiols. In this study, a versatile Eu-doped carbon dots (Eu-CDs) nanoprobe has been developed for the exceptionally sensitive and selective detection of GSH in biological fluids. The synthesized Eu-CDs exhibited high solubility in water, stability to salt and pH variations, and consistent fluorescence emission, boasting a quantum yield of 40.67 %. Following the optimization of detection parameters, a robust linear relationship for GSH was established in the concentration range of 0.0–50 μM, with a detection limit of 0.03 μM. The viability of the technique in actual samples was evaluated by successfully measuring the GSH in urine and human serum samples with a range recovery from 90.6 % to 98.5 %. Furthermore, Eu-CDs proved to be exceptionally suitable for imaging Hela cells owing to their low cytotoxicity and remarkable biocompatibility. As a result, the developed fluorescent biosensor demonstrated significant potential for widespread application in clinical diagnostic analyses.
期刊介绍:
The journal Carbon is an international multidisciplinary forum for communicating scientific advances in the field of carbon materials. It reports new findings related to the formation, structure, properties, behaviors, and technological applications of carbons. Carbons are a broad class of ordered or disordered solid phases composed primarily of elemental carbon, including but not limited to carbon black, carbon fibers and filaments, carbon nanotubes, diamond and diamond-like carbon, fullerenes, glassy carbon, graphite, graphene, graphene-oxide, porous carbons, pyrolytic carbon, and other sp2 and non-sp2 hybridized carbon systems. Carbon is the companion title to the open access journal Carbon Trends. Relevant application areas for carbon materials include biology and medicine, catalysis, electronic, optoelectronic, spintronic, high-frequency, and photonic devices, energy storage and conversion systems, environmental applications and water treatment, smart materials and systems, and structural and thermal applications.