脂质体组和转录物组的综合分析有助于深入了解过氧化氢处理对油脂红酵母 Sporobolomyces pararoseus 脂质合成的积极影响

IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY LWT - Food Science and Technology Pub Date : 2024-07-01 DOI:10.1016/j.lwt.2024.116389
Zhenyan Xie , Die Zhao , Bingxue Li , Nan Zeng , Dandan Wang , Xinxin Wang , Yunhao Sun , Mingwei Shao , Shuang Miao , Fei Feng , Ping Cheng , Guohui Yu , Chunji Li
{"title":"脂质体组和转录物组的综合分析有助于深入了解过氧化氢处理对油脂红酵母 Sporobolomyces pararoseus 脂质合成的积极影响","authors":"Zhenyan Xie ,&nbsp;Die Zhao ,&nbsp;Bingxue Li ,&nbsp;Nan Zeng ,&nbsp;Dandan Wang ,&nbsp;Xinxin Wang ,&nbsp;Yunhao Sun ,&nbsp;Mingwei Shao ,&nbsp;Shuang Miao ,&nbsp;Fei Feng ,&nbsp;Ping Cheng ,&nbsp;Guohui Yu ,&nbsp;Chunji Li","doi":"10.1016/j.lwt.2024.116389","DOIUrl":null,"url":null,"abstract":"<div><p>Microbial lipids are a group of promising renewable sources that are highly demanded by the food, feed, pharmaceutical, and biofuel industries. <em>Sporobolomyces pararoseus</em> is a well-studied oleaginous yeast that is usually considered superior for the industrial production of microbial lipids over other conventional microorganisms. The objective of this study was to investigate the specific effect of hydrogen peroxide treatment on the biosynthesis of microbial lipids in <em>S. pararoseus</em> via lipidomics and transcriptomics technologies. These results showed that hydrogen peroxide treatment significantly enhanced lipid production in <em>S. pararoseus</em> CJR, especially the triacylglycerols. The up-regulation of several key lipogenic genes might be one of the main reasons for this enhancement. Integrative analysis of the lipidome and transcriptome revealed that some key protein-encoding genes and transcription factors might play a crucial regulatory role in lipid biosynthesis under hydrogen peroxide stress. Together, the results presented herein not only provided a feasible fermentation process for boosting the yield of certain lipids but also deepened our understanding of the lipid biosynthesis in <em>S. pararoseus</em> CJR. This could serve as a molecular cornerstone for enhancing their yield through genetic engineering and then further facilitate commercial applications of microbial lipids synthesized by <em>S. pararoseus</em> in more industrial sectors.</p></div>","PeriodicalId":382,"journal":{"name":"LWT - Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0023643824006686/pdfft?md5=b0cd3185b04eff2cbda428fd45255849&pid=1-s2.0-S0023643824006686-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Integrated lipidomic and transcriptomic analyses provide insights into the positive effect of hydrogen peroxide treatment on lipid synthesis in oleaginous red yeast Sporobolomyces pararoseus\",\"authors\":\"Zhenyan Xie ,&nbsp;Die Zhao ,&nbsp;Bingxue Li ,&nbsp;Nan Zeng ,&nbsp;Dandan Wang ,&nbsp;Xinxin Wang ,&nbsp;Yunhao Sun ,&nbsp;Mingwei Shao ,&nbsp;Shuang Miao ,&nbsp;Fei Feng ,&nbsp;Ping Cheng ,&nbsp;Guohui Yu ,&nbsp;Chunji Li\",\"doi\":\"10.1016/j.lwt.2024.116389\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Microbial lipids are a group of promising renewable sources that are highly demanded by the food, feed, pharmaceutical, and biofuel industries. <em>Sporobolomyces pararoseus</em> is a well-studied oleaginous yeast that is usually considered superior for the industrial production of microbial lipids over other conventional microorganisms. The objective of this study was to investigate the specific effect of hydrogen peroxide treatment on the biosynthesis of microbial lipids in <em>S. pararoseus</em> via lipidomics and transcriptomics technologies. These results showed that hydrogen peroxide treatment significantly enhanced lipid production in <em>S. pararoseus</em> CJR, especially the triacylglycerols. The up-regulation of several key lipogenic genes might be one of the main reasons for this enhancement. Integrative analysis of the lipidome and transcriptome revealed that some key protein-encoding genes and transcription factors might play a crucial regulatory role in lipid biosynthesis under hydrogen peroxide stress. Together, the results presented herein not only provided a feasible fermentation process for boosting the yield of certain lipids but also deepened our understanding of the lipid biosynthesis in <em>S. pararoseus</em> CJR. This could serve as a molecular cornerstone for enhancing their yield through genetic engineering and then further facilitate commercial applications of microbial lipids synthesized by <em>S. pararoseus</em> in more industrial sectors.</p></div>\",\"PeriodicalId\":382,\"journal\":{\"name\":\"LWT - Food Science and Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0023643824006686/pdfft?md5=b0cd3185b04eff2cbda428fd45255849&pid=1-s2.0-S0023643824006686-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"LWT - Food Science and Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0023643824006686\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"LWT - Food Science and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0023643824006686","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

微生物脂类是一类前景广阔的可再生资源,在食品、饲料、制药和生物燃料工业中需求量很大。副嗜油孢子酵母是一种已被充分研究的含油酵母,通常被认为在微生物脂类的工业生产中优于其他传统微生物。本研究的目的是通过脂质组学和转录组学技术研究过氧化氢处理对副嗜酸梭菌微生物脂质生物合成的具体影响。结果表明,过氧化氢处理显著提高了副猪痢疾杆菌 CJR 的脂质产量,尤其是三酰甘油。几个关键生脂基因的上调可能是导致这种提高的主要原因之一。对脂质体和转录组的综合分析表明,一些关键的蛋白编码基因和转录因子可能在过氧化氢胁迫下的脂质生物合成过程中发挥着重要的调控作用。总之,本文介绍的结果不仅为提高某些脂质的产量提供了可行的发酵工艺,而且加深了我们对 S. pararoseus CJR 脂质生物合成的理解。这可以作为通过基因工程提高其产量的分子基石,进而进一步促进由 S. pararoseus 合成的微生物脂类在更多工业领域的商业应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Integrated lipidomic and transcriptomic analyses provide insights into the positive effect of hydrogen peroxide treatment on lipid synthesis in oleaginous red yeast Sporobolomyces pararoseus

Microbial lipids are a group of promising renewable sources that are highly demanded by the food, feed, pharmaceutical, and biofuel industries. Sporobolomyces pararoseus is a well-studied oleaginous yeast that is usually considered superior for the industrial production of microbial lipids over other conventional microorganisms. The objective of this study was to investigate the specific effect of hydrogen peroxide treatment on the biosynthesis of microbial lipids in S. pararoseus via lipidomics and transcriptomics technologies. These results showed that hydrogen peroxide treatment significantly enhanced lipid production in S. pararoseus CJR, especially the triacylglycerols. The up-regulation of several key lipogenic genes might be one of the main reasons for this enhancement. Integrative analysis of the lipidome and transcriptome revealed that some key protein-encoding genes and transcription factors might play a crucial regulatory role in lipid biosynthesis under hydrogen peroxide stress. Together, the results presented herein not only provided a feasible fermentation process for boosting the yield of certain lipids but also deepened our understanding of the lipid biosynthesis in S. pararoseus CJR. This could serve as a molecular cornerstone for enhancing their yield through genetic engineering and then further facilitate commercial applications of microbial lipids synthesized by S. pararoseus in more industrial sectors.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
LWT - Food Science and Technology
LWT - Food Science and Technology 工程技术-食品科技
CiteScore
11.80
自引率
6.70%
发文量
1724
审稿时长
65 days
期刊介绍: LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.
期刊最新文献
Comparative analysis of drying characteristics, physicochemical properties and volatile flavor components of Dendrocalamus brandisii shoots under four drying techniques Isolation, purification and characterization of antioxidant peptides from Spirulina platensis extracts co-fermentation with Thermus thermophilus HB27 and Saccharomyces cerevisiae CH006 Regulation of tea polyphenols in gluten-glucose Maillard reaction: Evaluation and analysis Investigation of different nanoemulsion coatings to prevent anthracnose and maintain quality parameters during storage of Irwin mango Study on ferritin glycation with dextran: Physicochemical characterization and its application in the delivery of resveratrol
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1