使用水杨酸、过氧化氢和热水处理法减少受感染茎插条上的木薯花叶病毒

IF 1.1 4区 农林科学 Q3 PLANT SCIENCES Journal of Phytopathology Pub Date : 2024-06-24 DOI:10.1111/jph.13347
Mercy W. Kung'u, Evans N. Nyaboga, Chrissie Rey
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引用次数: 0

摘要

木薯马赛克病(CMD)给非洲的木薯生产造成重大损失。该病由几种木薯花叶病毒(CMG)引起,通过使用受感染的植物材料传播。由于标准的植物保护措施无法控制这种疾病,受感染的植物在其整个无性生殖周期中都会受到感染。因此,重要的是要开发成本低廉的田间方法,使小规模农户能够轻松采用这些方法来消毒受 geminiviruses 感染的用作种植材料的茎插条。本研究旨在通过使用水杨酸(SA)、过氧化氢(H2O2)和热水(HW)处理,消除受感染木薯茎插条中的 CMGs。八个受 CMD 感染的木薯茎插条在玻璃温室中插植之前,分别用水杨酸(1.25、2.5 和 5 mM)、过氧化氢(0.5%、1.0% 和 1.5%)或热水(50 和 55°C)在不同的暴露时间下进行预处理,以评估随后的植物生长和 geminivirus 检测情况。根据用于不同处理的木薯品种,50°C 热水处理 5 分钟和 1.0% H2O2 处理 12 小时对消除 CMGs 最有效,效率分别为 81.7% 和 77.8%。浓度为 5 mM 的水杨酸持续 6 小时可消除 65.1% 插条上的病毒。本文测试的方法有可能为小农户和木薯产业生产出显著降低 CMD 风险的种植材料,以满足他们日益增长的需求。它还能促进全球种质资源交流,用于保护和育种计划。
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Reduction of cassava mosaic geminiviruses from infected stem cuttings using salicylic acid, hydrogen peroxide and hot water treatment

Cassava mosaic disease (CMD) causes significant losses in cassava production in Africa. The disease is caused by several cassava mosaic geminiviruses (CMGs) and spread through the use of infected plant materials. The infected plants remain infected throughout their vegetative lifecycle as the disease cannot be controlled by standard plant protection measures. Therefore, it is important to develop inexpensive field-based methods that can be easily adopted by small-scale farmers to sanitize geminiviruses-infected stem cuttings used as planting material. This study aimed at eliminating CMGs from infected cassava stem cuttings by use of salicylic acid (SA), hydrogen peroxide (H2O2) and hot water (HW) treatments. Eight varieties of CMD-infected stem cuttings were pretreated with SA (1.25, 2.5 and 5 mM), H2O2 (0.5%, 1.0% and 1.5%) or hot water (50 and 55°C) at different exposure times before establishment in the glasshouse for evaluation of subsequent plant growth and geminivirus detection. Based on the cassava varieties used for the different treatments, treatment with hot water at 50°C for 5 min and 1.0% H2O2 for 12 h were the most effective in eliminating CMGs with an efficiency of 81.7% and 77.8%, respectively. Salicylic acid at a concentration of 5 mM for 6 h eliminated the viruses in 65.1% of the cuttings. The methods tested herein have the potential for producing planting materials with significantly reduced CMD risk for smallholder farmers and the cassava industry to meet their increasing demand. It could also contribute to the global exchange of germplasm for conservation and breeding programs.

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来源期刊
Journal of Phytopathology
Journal of Phytopathology 生物-植物科学
CiteScore
2.90
自引率
0.00%
发文量
88
审稿时长
4-8 weeks
期刊介绍: Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
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