对胃癌发生过程中不同胃微生境下胃黏膜细菌和血浆代谢物特征的多组学综合分析

IF 4.9 2区 医学 Q2 CELL BIOLOGY Cellular Oncology Pub Date : 2024-07-04 DOI:10.1007/s13402-024-00965-3
Bingsen Wang, Jiahui Luan, Weidong Zhao, Junbao Yu, Anqing Li, Xinxin Li, Xiaoqin Zhong, Hongyun Cao, Ruicai Wang, Bo Liu, Shiyong Lu, Mei Shi
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引用次数: 0

摘要

目的:作为微环境的重要组成部分,胃微生物群及其代谢产物与肿瘤的发生、发展和转移有关。然而,胃微生物群与胃癌发病之间的关系尚不清楚。本研究探讨了胃黏膜微生物组和代谢物在胃癌发生中作为致病因素的作用:方法:对来自不同胃微生境的胃活检样本(n = 70)进行 16S rRNA 基因测序,并对血液样本(n = 95)进行非靶向代谢组(气相色谱-质谱法,GC-MS)分析。采用各种生物信息学方法对数据集进行了分析:结果:在胃癌发生过程中,微生物群的多样性和群落组成发生了明显变化。幽门螺杆菌的高定植率改变了与胃炎和胃癌相关的微生物群的整体多样性和组成。最重要的是,对微生物群功能特征的分析表明,硝酸还原酶基因在肿瘤微生物群中显著富集,而尿素酶产生基因在幽门螺杆菌阳性患者的微生物群中显著富集。由81种代谢物组成的小组被用来区分胃癌患者和胃炎患者,由15种代谢物组成的小组被用来区分幽门螺杆菌阳性患者和幽门螺杆菌阴性患者。接收操作者特征(ROC)曲线分析确定了一系列胃微生物和血浆代谢物是胃癌的潜在生物标志物:本研究发现了一系列可能在胃癌发生过程中发挥重要作用的特征,这些特征有可能被用作诊断和监测微创胃癌患者的生物标志物。
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Comprehensive multiomics analysis of the signatures of gastric mucosal bacteria and plasma metabolites across different stomach microhabitats in the development of gastric cancer.

Purpose: As an important component of the microenvironment, the gastric microbiota and its metabolites are associated with tumour occurrence, progression, and metastasis. However, the relationship between the gastric microbiota and the development of gastric cancer is unclear. The present study investigated the role of the gastric mucosa microbiome and metabolites as aetiological factors in gastric carcinogenesis.

Methods: Gastric biopsies from different stomach microhabitats (n = 70) were subjected to 16S rRNA gene sequencing, and blood samples (n = 95) were subjected to untargeted metabolome (gas chromatography‒mass spectrometry, GC‒MS) analyses. The datasets were analysed using various bioinformatics approaches.

Results: The microbiota diversity and community composition markedly changed during gastric carcinogenesis. High Helicobacter. pylori colonization modified the overall diversity and composition of the microbiota associated with gastritis and cancer in the stomach. Most importantly, analysis of the functional features of the microbiota revealed that nitrate reductase genes were significantly enriched in the tumoral microbiota, while urease-producing genes were significantly enriched in the microbiota of H. pylori-positive patients. A panel of 81 metabolites was constructed to discriminate gastric cancer patients from gastritis patients, and a panel of 15 metabolites was constructed to discriminate H. pylori-positive patients from H. pylori-negative patients. receiver operator characteristic (ROC) curve analysis identified a series of gastric microbes and plasma metabolites as potential biomarkers of gastric cancer.

Conclusion: The present study identified a series of signatures that may play important roles in gastric carcinogenesis and have the potential to be used as biomarkers for diagnosis and for the surveillance of gastric cancer patients with minimal invasiveness.

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来源期刊
Cellular Oncology
Cellular Oncology ONCOLOGY-CELL BIOLOGY
CiteScore
10.30
自引率
1.50%
发文量
86
审稿时长
12 months
期刊介绍: The Official Journal of the International Society for Cellular Oncology Focuses on translational research Addresses the conversion of cell biology to clinical applications Cellular Oncology publishes scientific contributions from various biomedical and clinical disciplines involved in basic and translational cancer research on the cell and tissue level, technical and bioinformatics developments in this area, and clinical applications. This includes a variety of fields like genome technology, micro-arrays and other high-throughput techniques, genomic instability, SNP, DNA methylation, signaling pathways, DNA organization, (sub)microscopic imaging, proteomics, bioinformatics, functional effects of genomics, drug design and development, molecular diagnostics and targeted cancer therapies, genotype-phenotype interactions. A major goal is to translate the latest developments in these fields from the research laboratory into routine patient management. To this end Cellular Oncology forms a platform of scientific information exchange between molecular biologists and geneticists, technical developers, pathologists, (medical) oncologists and other clinicians involved in the management of cancer patients. In vitro studies are preferentially supported by validations in tumor tissue with clinicopathological associations.
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