Claudia Reyes-San-Martin , Arturo Elías-Llumbet , Elkin Escobar-Chaves , Marcia Manterola , Aldona Mzyk , Romana Schirhagl
{"title":"基于金刚石的自由基迁移人类乳腺癌细胞量子传感技术","authors":"Claudia Reyes-San-Martin , Arturo Elías-Llumbet , Elkin Escobar-Chaves , Marcia Manterola , Aldona Mzyk , Romana Schirhagl","doi":"10.1016/j.carbon.2024.119405","DOIUrl":null,"url":null,"abstract":"<div><p>Cell migration is a crucial parameter for disease progression in cancer. Reactive oxygen species (ROS) levels are involved in the regulation of the migration process, however, the exact role of free radical generation and where it occurs is unknown. Here we use a diamond-based quantum sensing technique to detect free radicals during cancer cell migration in real time with subcellular resolution. We investigated metastatic MDA-MB-231 human breast cancer cells and observed free radical formation after 16 h of starvation and 24 h of migration under low-serum conditions. Intracellular diamond dynamics were monitored at different migration points (0, 12, and 24 h), and cell morphology was evaluated. Additionally, the number of focal adhesions was analyzed as an indicator of the migratory potential of the cells. We further measured free radical generation under nicotinamide adenine dinucleotide phosphate hydrogen NADPH oxidases 2 (NOX2) inhibition by apocynin. We found that free radical levels decreased after 24 h treatment with 36 μg/mL apocynin while the levels of ROS and the migratory capacity of the cells increased. Our results evidence the complexity of the redox regulation in migrating cancer cells and offer a novel approach to specifically and locally interrogate pivotal players of the oxidative network behind metastatic success.</p></div>","PeriodicalId":262,"journal":{"name":"Carbon","volume":null,"pages":null},"PeriodicalIF":10.5000,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0008622324006249/pdfft?md5=59a4c8d8cd523f21da2e675ca09ad8df&pid=1-s2.0-S0008622324006249-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Diamond-based quantum sensing of free radicals in migrating human breast cancer cells\",\"authors\":\"Claudia Reyes-San-Martin , Arturo Elías-Llumbet , Elkin Escobar-Chaves , Marcia Manterola , Aldona Mzyk , Romana Schirhagl\",\"doi\":\"10.1016/j.carbon.2024.119405\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Cell migration is a crucial parameter for disease progression in cancer. Reactive oxygen species (ROS) levels are involved in the regulation of the migration process, however, the exact role of free radical generation and where it occurs is unknown. Here we use a diamond-based quantum sensing technique to detect free radicals during cancer cell migration in real time with subcellular resolution. We investigated metastatic MDA-MB-231 human breast cancer cells and observed free radical formation after 16 h of starvation and 24 h of migration under low-serum conditions. Intracellular diamond dynamics were monitored at different migration points (0, 12, and 24 h), and cell morphology was evaluated. Additionally, the number of focal adhesions was analyzed as an indicator of the migratory potential of the cells. We further measured free radical generation under nicotinamide adenine dinucleotide phosphate hydrogen NADPH oxidases 2 (NOX2) inhibition by apocynin. We found that free radical levels decreased after 24 h treatment with 36 μg/mL apocynin while the levels of ROS and the migratory capacity of the cells increased. Our results evidence the complexity of the redox regulation in migrating cancer cells and offer a novel approach to specifically and locally interrogate pivotal players of the oxidative network behind metastatic success.</p></div>\",\"PeriodicalId\":262,\"journal\":{\"name\":\"Carbon\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":10.5000,\"publicationDate\":\"2024-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0008622324006249/pdfft?md5=59a4c8d8cd523f21da2e675ca09ad8df&pid=1-s2.0-S0008622324006249-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Carbon\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0008622324006249\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbon","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0008622324006249","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Diamond-based quantum sensing of free radicals in migrating human breast cancer cells
Cell migration is a crucial parameter for disease progression in cancer. Reactive oxygen species (ROS) levels are involved in the regulation of the migration process, however, the exact role of free radical generation and where it occurs is unknown. Here we use a diamond-based quantum sensing technique to detect free radicals during cancer cell migration in real time with subcellular resolution. We investigated metastatic MDA-MB-231 human breast cancer cells and observed free radical formation after 16 h of starvation and 24 h of migration under low-serum conditions. Intracellular diamond dynamics were monitored at different migration points (0, 12, and 24 h), and cell morphology was evaluated. Additionally, the number of focal adhesions was analyzed as an indicator of the migratory potential of the cells. We further measured free radical generation under nicotinamide adenine dinucleotide phosphate hydrogen NADPH oxidases 2 (NOX2) inhibition by apocynin. We found that free radical levels decreased after 24 h treatment with 36 μg/mL apocynin while the levels of ROS and the migratory capacity of the cells increased. Our results evidence the complexity of the redox regulation in migrating cancer cells and offer a novel approach to specifically and locally interrogate pivotal players of the oxidative network behind metastatic success.
期刊介绍:
The journal Carbon is an international multidisciplinary forum for communicating scientific advances in the field of carbon materials. It reports new findings related to the formation, structure, properties, behaviors, and technological applications of carbons. Carbons are a broad class of ordered or disordered solid phases composed primarily of elemental carbon, including but not limited to carbon black, carbon fibers and filaments, carbon nanotubes, diamond and diamond-like carbon, fullerenes, glassy carbon, graphite, graphene, graphene-oxide, porous carbons, pyrolytic carbon, and other sp2 and non-sp2 hybridized carbon systems. Carbon is the companion title to the open access journal Carbon Trends. Relevant application areas for carbon materials include biology and medicine, catalysis, electronic, optoelectronic, spintronic, high-frequency, and photonic devices, energy storage and conversion systems, environmental applications and water treatment, smart materials and systems, and structural and thermal applications.