Zihao Li, Yongbin Gu, Zixuan Hu, Furi Zhang, Yi Li, Mengqi Tian, Weijun Tian, Weirong Tian, Jiaru Li
{"title":"转录组和代谢组的综合分析揭示了二氢茉莉酸甲酯对云南巴黎绿变种生理指标和多花素合成的促进作用","authors":"Zihao Li, Yongbin Gu, Zixuan Hu, Furi Zhang, Yi Li, Mengqi Tian, Weijun Tian, Weirong Tian, Jiaru Li","doi":"10.1007/s00344-024-11413-9","DOIUrl":null,"url":null,"abstract":"<p>Jasmonic acids, their effects on plant resistance and metabolites, have been extensively researched. However, methyl dihydrojasmonate (MDJ), a cost-effective and safe substitute for jasmonic acids, lacks sufficient research. This study presents a comprehensive analysis the impact of MDJ on <i>Paris polyphylla</i> var. <i>yunnanensis.</i> The present study aimed to enhance the accumulation of steroidal saponins (polyphyllin I, II, III, D, V, VI, VII, H) in <i>Paris polyphylla</i> var<i>. yunnanensis</i> which is a medicinal plant that contains medicinal steroidal saponins. To achieve this, different concentrations of MDJ were applied to <i>Paris polyphylla</i> var<i>. yunnanensis</i>. The study examined the impact of MDJ on physiology and polyphyllins. The optimal treatment concentration was identified, and the effects of MDJ on physiological metabolism and the expression pattern of related genes were explored. The results indicated that MDJ treatment significantly increased the levels of photosynthetic pigments, antioxidant enzymes, osmoregulators peaked at M2. Moreover, MDJ enhanced the accumulation of polyphyllins in both the leaves and rhizomes peaked at M2, particularly the sum of four polyphyllins (polyphyllin I, II, VI, VII) stated in the Chinese Pharmacopoeia, which increased by 45.65% following treatment. Based on transcriptome analysis, we revealed the polyphyllins-related genes by WGCNA. Furthermore, the majority of genes related to steroidal saponins were up-regulated following MDJ treatment. In the future, we recommend treating <i>Paris polyphylla</i> var<i>. yunnanensis</i> with suitable MDJ concentrations in production to efficiently enhance the yield of steroidal saponins.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integrated Analysis of Transcriptome and Metabolome Reveal the Enhancement of Methyl Dihydrojasmonate on Physiological Indicators and Polyphyllins Biosynthesis in Paris polyphylla var. yunnanensis\",\"authors\":\"Zihao Li, Yongbin Gu, Zixuan Hu, Furi Zhang, Yi Li, Mengqi Tian, Weijun Tian, Weirong Tian, Jiaru Li\",\"doi\":\"10.1007/s00344-024-11413-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Jasmonic acids, their effects on plant resistance and metabolites, have been extensively researched. However, methyl dihydrojasmonate (MDJ), a cost-effective and safe substitute for jasmonic acids, lacks sufficient research. This study presents a comprehensive analysis the impact of MDJ on <i>Paris polyphylla</i> var. <i>yunnanensis.</i> The present study aimed to enhance the accumulation of steroidal saponins (polyphyllin I, II, III, D, V, VI, VII, H) in <i>Paris polyphylla</i> var<i>. yunnanensis</i> which is a medicinal plant that contains medicinal steroidal saponins. To achieve this, different concentrations of MDJ were applied to <i>Paris polyphylla</i> var<i>. yunnanensis</i>. The study examined the impact of MDJ on physiology and polyphyllins. The optimal treatment concentration was identified, and the effects of MDJ on physiological metabolism and the expression pattern of related genes were explored. The results indicated that MDJ treatment significantly increased the levels of photosynthetic pigments, antioxidant enzymes, osmoregulators peaked at M2. Moreover, MDJ enhanced the accumulation of polyphyllins in both the leaves and rhizomes peaked at M2, particularly the sum of four polyphyllins (polyphyllin I, II, VI, VII) stated in the Chinese Pharmacopoeia, which increased by 45.65% following treatment. Based on transcriptome analysis, we revealed the polyphyllins-related genes by WGCNA. Furthermore, the majority of genes related to steroidal saponins were up-regulated following MDJ treatment. In the future, we recommend treating <i>Paris polyphylla</i> var<i>. yunnanensis</i> with suitable MDJ concentrations in production to efficiently enhance the yield of steroidal saponins.</p>\",\"PeriodicalId\":16842,\"journal\":{\"name\":\"Journal of Plant Growth Regulation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Growth Regulation\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00344-024-11413-9\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Growth Regulation","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00344-024-11413-9","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Integrated Analysis of Transcriptome and Metabolome Reveal the Enhancement of Methyl Dihydrojasmonate on Physiological Indicators and Polyphyllins Biosynthesis in Paris polyphylla var. yunnanensis
Jasmonic acids, their effects on plant resistance and metabolites, have been extensively researched. However, methyl dihydrojasmonate (MDJ), a cost-effective and safe substitute for jasmonic acids, lacks sufficient research. This study presents a comprehensive analysis the impact of MDJ on Paris polyphylla var. yunnanensis. The present study aimed to enhance the accumulation of steroidal saponins (polyphyllin I, II, III, D, V, VI, VII, H) in Paris polyphylla var. yunnanensis which is a medicinal plant that contains medicinal steroidal saponins. To achieve this, different concentrations of MDJ were applied to Paris polyphylla var. yunnanensis. The study examined the impact of MDJ on physiology and polyphyllins. The optimal treatment concentration was identified, and the effects of MDJ on physiological metabolism and the expression pattern of related genes were explored. The results indicated that MDJ treatment significantly increased the levels of photosynthetic pigments, antioxidant enzymes, osmoregulators peaked at M2. Moreover, MDJ enhanced the accumulation of polyphyllins in both the leaves and rhizomes peaked at M2, particularly the sum of four polyphyllins (polyphyllin I, II, VI, VII) stated in the Chinese Pharmacopoeia, which increased by 45.65% following treatment. Based on transcriptome analysis, we revealed the polyphyllins-related genes by WGCNA. Furthermore, the majority of genes related to steroidal saponins were up-regulated following MDJ treatment. In the future, we recommend treating Paris polyphylla var. yunnanensis with suitable MDJ concentrations in production to efficiently enhance the yield of steroidal saponins.
期刊介绍:
The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches.
The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress.
In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports.
The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.