Jin Cheng, Zijun Zeng, Riaz Muhammad, Zeinab El-Desouki, Cuncang Jiang
{"title":"脯氨酸抗硼缺乏对不同生长阶段棉花(Gossypium hirsutum L.)光合作用和脯氨酸代谢物的保护作用","authors":"Jin Cheng, Zijun Zeng, Riaz Muhammad, Zeinab El-Desouki, Cuncang Jiang","doi":"10.1007/s00344-024-11403-x","DOIUrl":null,"url":null,"abstract":"<p>Boron (B) is the most common trace element deficiency of cotton (<i>Gossypium hirsutum</i> L.) plantation in China. Whereas the narrow range of the deficiency and toxicity restrict the application of B, proline (Pro) mitigates many kinds of stress damage in plants, including B deficiency. How the pro-regulation of pro-metabolites affects plant growth, photosynthesis and leaf structure under boron (B) deficiency condition remains poorly understood. Here, this study investigates the effects of exogenous proline on the growth of Pro metabolites in cotton (E Kang 10) under B deficiency at different growth stages. We applied three Pro concentrations (0, 1.5 and 3 mg/L) with two B concentrations (0.1, 0.5 mg/L), with a total of six treatments. The addition of exogenous Pro at 1.5 mg/L significantly promoted the accumulation of B in leaves and increased the dry and fresh weight of various parts of the plant under low B stress. Additionally, the content of MDA was reduced, while the content of soluble protein increased in cotton plants under B<sub>0.1</sub> treatment after the addition of Pro. Both 1.5 and 3 mg/L of Pro increased the ratio of chlorophyll a/b under B stress. In the late stage, the synthesis of Pro is mainly promoted by regulating the activities of Arg, P5CS, P5CR and ProDH. Under two B levels, the application of Pro significantly reduced the Pro content in roots at both growth stages, which was related to the decrease in Arg, Glu, GSA and P5CS activities. Additionally, exogenous applicable Pro has a better-alleviating effect on low B stress of cotton seedlings.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protective Effect of Proline Against Boron Deficiency on Photosynthesis and Proline Metabolites in Cotton (Gossypium hirsutum L.) at Different Growth Stages\",\"authors\":\"Jin Cheng, Zijun Zeng, Riaz Muhammad, Zeinab El-Desouki, Cuncang Jiang\",\"doi\":\"10.1007/s00344-024-11403-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Boron (B) is the most common trace element deficiency of cotton (<i>Gossypium hirsutum</i> L.) plantation in China. Whereas the narrow range of the deficiency and toxicity restrict the application of B, proline (Pro) mitigates many kinds of stress damage in plants, including B deficiency. How the pro-regulation of pro-metabolites affects plant growth, photosynthesis and leaf structure under boron (B) deficiency condition remains poorly understood. Here, this study investigates the effects of exogenous proline on the growth of Pro metabolites in cotton (E Kang 10) under B deficiency at different growth stages. We applied three Pro concentrations (0, 1.5 and 3 mg/L) with two B concentrations (0.1, 0.5 mg/L), with a total of six treatments. The addition of exogenous Pro at 1.5 mg/L significantly promoted the accumulation of B in leaves and increased the dry and fresh weight of various parts of the plant under low B stress. Additionally, the content of MDA was reduced, while the content of soluble protein increased in cotton plants under B<sub>0.1</sub> treatment after the addition of Pro. Both 1.5 and 3 mg/L of Pro increased the ratio of chlorophyll a/b under B stress. In the late stage, the synthesis of Pro is mainly promoted by regulating the activities of Arg, P5CS, P5CR and ProDH. Under two B levels, the application of Pro significantly reduced the Pro content in roots at both growth stages, which was related to the decrease in Arg, Glu, GSA and P5CS activities. Additionally, exogenous applicable Pro has a better-alleviating effect on low B stress of cotton seedlings.</p>\",\"PeriodicalId\":16842,\"journal\":{\"name\":\"Journal of Plant Growth Regulation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Growth Regulation\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00344-024-11403-x\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Growth Regulation","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00344-024-11403-x","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
硼(B)是中国棉花(Gossypium hirsutum L.)种植中最常见的微量元素缺乏症。硼元素的缺乏范围窄、毒性大,限制了硼元素的应用,而脯氨酸(Pro)能减轻植物的多种胁迫伤害,包括硼元素缺乏。硼(B)缺乏条件下,脯氨酸代谢产物的脯氨酸调节如何影响植物的生长、光合作用和叶片结构,目前尚不清楚。在此,本研究调查了外源脯氨酸对棉花(E Kang 10)在不同生长阶段缺硼情况下脯代谢物生长的影响。我们采用了三种 Pro 浓度(0、1.5 和 3 mg/L)和两种 B 浓度(0.1、0.5 mg/L),共六种处理。在低硼胁迫下,添加 1.5 mg/L 的外源 Pro 能显著促进叶片中硼的积累,并增加植株各部分的干重和鲜重。此外,添加 Pro 后,B0.1 处理下棉花植株的 MDA 含量降低,而可溶性蛋白含量增加。在硼胁迫下,1.5 毫克/升和 3 毫克/升的 Pro 都提高了叶绿素 a/b 的比率。在后期,主要通过调节 Arg、P5CS、P5CR 和 ProDH 的活性来促进 Pro 的合成。在两种 B 水平下,施用 Pro 会显著降低两个生长阶段根中的 Pro 含量,这与 Arg、Glu、GSA 和 P5CS 活性的降低有关。此外,外源应用 Pro 对棉花幼苗的低 B 胁迫有较好的缓解作用。
Protective Effect of Proline Against Boron Deficiency on Photosynthesis and Proline Metabolites in Cotton (Gossypium hirsutum L.) at Different Growth Stages
Boron (B) is the most common trace element deficiency of cotton (Gossypium hirsutum L.) plantation in China. Whereas the narrow range of the deficiency and toxicity restrict the application of B, proline (Pro) mitigates many kinds of stress damage in plants, including B deficiency. How the pro-regulation of pro-metabolites affects plant growth, photosynthesis and leaf structure under boron (B) deficiency condition remains poorly understood. Here, this study investigates the effects of exogenous proline on the growth of Pro metabolites in cotton (E Kang 10) under B deficiency at different growth stages. We applied three Pro concentrations (0, 1.5 and 3 mg/L) with two B concentrations (0.1, 0.5 mg/L), with a total of six treatments. The addition of exogenous Pro at 1.5 mg/L significantly promoted the accumulation of B in leaves and increased the dry and fresh weight of various parts of the plant under low B stress. Additionally, the content of MDA was reduced, while the content of soluble protein increased in cotton plants under B0.1 treatment after the addition of Pro. Both 1.5 and 3 mg/L of Pro increased the ratio of chlorophyll a/b under B stress. In the late stage, the synthesis of Pro is mainly promoted by regulating the activities of Arg, P5CS, P5CR and ProDH. Under two B levels, the application of Pro significantly reduced the Pro content in roots at both growth stages, which was related to the decrease in Arg, Glu, GSA and P5CS activities. Additionally, exogenous applicable Pro has a better-alleviating effect on low B stress of cotton seedlings.
期刊介绍:
The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches.
The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress.
In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports.
The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.