Yuchen Zhou, Wanchun Chen, Guangyuan Wang, Zhenfeng Lei, Mei Zhang and Yanxia Li
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引用次数: 0
摘要
研究人员提出了一种基于掺氮激光直接石墨烯(N-LIG)/金电极的无标记免疫传感器,用于检测甲型 H1N1 流感病毒。利用激光辐照的瞬时高温,三聚氰胺分解产生的 N 原子滴落到石墨烯电极表面,从而获得了导电率更高的 N-LIG。N 原子的掺杂为 LIG 微电极提供了大量的活性位点。结合金氧化物的电沉积和抗体的共价交联,构建了一个简单、高灵敏度和高稳定性的免疫传感界面。该 H1N1 流感病毒免疫传感器的检测范围为 0.01 fg mL-1 至 10 ng mL-1,检测限低至 0.004 fg mL-1。所构建的传感器具有超高灵敏度和良好的选择性,可用于复杂生物样品的分析,在防止流感大规模传播方面具有潜在的应用前景。利用 N-LIG 电极的特性将为开发用于健康和环境应用的便携式电化学生物传感器提供机会。
A label-free H1N1 influenza virus immunosensor based on an N-LIG/Au laser induced graphene microelectrode†
A label-free immunosensor based on an N-doped laser direct graphene (N-LIG)/Au electrode was proposed for H1N1 influenza virus detection. By utilizing the instantaneous high temperature of laser irradiation, N atoms are generated by the decomposition of melamine dripped onto the surface of an LIG electrode to obtain N-LIG with higher conductivity. The doping of N atoms provides a large number of active sites for LIG microelectrodes. Combined with the electrodeposition of Au NPs, and covalently crosslinking antibodies, a simple, highly sensitive and stable immunosensing interface is constructed. The proposed H1N1 influenza virus immunosensor has a detection range of 0.01 fg mL−1 to 10 ng mL−1 with a detection limit as low as 0.004 fg mL−1. The constructed sensor has ultra-high sensitivity and good selectivity and can be used for complex biological sample analysis, with potential application prospects in preventing the large-scale spread of influenza. Taking advantage of N-LIG electrode's properties will provide opportunities for developing portable electrochemical biosensors for health and environmental applications.