Weiqin Zhao, Ruichen Zhai, Qianxiao Chen, Chun Huang, Haojia Li, Youyu Zhu, Yingfeng Duan, Jie Gao
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引用次数: 0
摘要
谷胱甘肽(GSH)通常被用作多种疾病的诊断生物标志物。本研究以火龙果皮制备的碳量子点(D-CQDs)和 T-Hg(Ⅱ)-T 错配为基础,开发了一种检测 GSH 的双模式生物传感器。该系统由两条单链 DNA(ssDNA)组成。DNA1 是富含 T 的序列;DNA2 连接到链霉亲和素包被的磁珠上,由富含 T 和富含 G 的片段组成。由于 Hg(Ⅱ)的存在,两个 ssDNA 的富 T 片段之间形成了 T-Hg(Ⅱ)-T 错配。在有 GSH 存在的情况下,Hg(Ⅱ) 从 dsDNA 上脱离,并与 GSH 结合形成新的复合物。富含 G 的片段与 D-CQDs 上脱落的 hemin 结合,形成 G-四叠体/hemin 复合物。此时,在荧光模式下,被hemin淬灭的D-CQDs荧光可以恢复。在比色模式下,磁珠分离后,利用 G-quadruplex/hemin 复合物的过氧化物样活性催化 ABTS 氧化,可产生视觉信号。该生物传感器在荧光模式和比色模式下均具有良好的选择性和灵敏度,对 GSH 的检测限分别为 0.089 μM 和 0.26 μM。此外,该双模式生物传感器在检测 GSH 方面具有良好的应用前景。
Detection of GSH with a dual-mode biosensor based on carbon quantum dots prepared from dragon fruit peel and T-Hg(Ⅱ)-T mismatch
Glutathione (GSH) was commonly used as a diagnostic biomarker for many diseases. In this study, based on carbon quantum dots prepared from dragon fruit peel (D-CQDs) and T-Hg(Ⅱ)-T mismatch, a dual-mode biosensor was developed for the detection of GSH. This system consists of two single-stranded DNA (ssDNA). DNA1 was the T-rich sequence; DNA2 was attached to streptavidin-coated magnetic beads, and consisted of T-rich and G-rich fragment. Due to the presence of Hg(Ⅱ), the T-Hg(Ⅱ)-T mismatch was formed between T-rich fragment of two ssDNA. In the presence of GSH, Hg(Ⅱ) detached from dsDNA and bound with GSH to form a new complex. The G-rich fragment assembled with the hemin shed from D-CQDs to form the G-quadruplex/hemin complex. At this time, in fluorescence mode, the fluorescence of D-CQDs quenched by hemin could be restored. In colorimetric mode, after the magnetic beads separate, a visual signal could be produced by catalyzing the oxidation of ABTS using the peroxide-like activity of G-quadruplex/hemin complex. This biosensor in fluorescence mode and colorimetric mode both had excellent selectivity and sensitivity, and the limit of detection was 0.089 μM and 0.26 μM for GSH, respectively. Moreover, this proposed dual-mode biosensor had good application prospects for detection of GSH.