Jiayi Yang, Wei Zhang, Binghao Lin, Shuming Mao, Guangyao Liu, Kai Tan and Jiahao Tang*,
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引用次数: 0
摘要
本研究探讨了如何对钛(Ti)植入物进行表面改性,以增强早期阶段的骨结合,从而降低骨质疏松性骨折内固定的失败率,因为骨质疏松性骨折本身就会降低骨量和骨强度。我们采用了逐层电装配技术,将含透明质酸/壳聚糖的梓醇多层膜沉积到钛植入物表面。为了评估梓醇涂层 Ti 植入体的体外骨诱导效应,我们采用了具有强大成骨细胞分化能力的小鼠前成骨细胞系 MC3T3-E1。此外,还通过在 Sprague-Dawley 大鼠的股骨髓内植入对这些植入物的性能进行了体内评估。工程植入物能有效调节梓醇的释放,在植入初期促进骨形成。体外研究结果表明,涂有 catalpol 的钛表面提高了 ALP 活性、CCK-8 测定的细胞增殖和 WB 分析的成骨蛋白表达,超过了未涂覆的钛组(P < 0.05)。体内显微计算机断层扫描(CT)和组织学分析表明,在植入后 14 天,梓醇涂层 Ti 显著促进了骨质疏松大鼠新骨的形成和重塑。这项研究为制造生物功能钛植入体解决骨质疏松症问题提供了一种全面而简单的方法。
Enhancement of Local Osseointegration and Implant Stability of Titanium Implant in Osteoporotic Rats by Biomimetic Multilayered Structures Containing Catalpol
This study examined the surface modification of titanium (Ti) implants to enhance early-stage osseointegration, which reduced the failure rate of internal fixation in osteoporotic fractures that inherently decrease in bone mass and strength. We employed a layer-by-layer electroassembly technique to deposit catalpol-containing hyaluronic acid/chitosan multilayers onto the surface of Ti implants. To evaluate the in vitro osteoinductive effects of catalpol-coated Ti implants, the robust osteoblast differentiation capacity of the murine preosteoblast cell line, MC3T3-E1, was employed. Furthermore, the performance of these implants was evaluated in vivo through femoral intramedullary implantation in Sprague–Dawley rats. The engineered implant effectively regulated catalpol release, promoting increased bone formation during the initial stages of implantation. The in vitro findings demonstrated that catalpol-coated Ti surfaces boosted ALP activity, cell proliferation as measured by CCK-8, and osteogenic protein expression via WB analysis, surpassing the uncoated Ti group (P < 0.05). In vivo micro-computed tomography (CT) and histological analyses revealed that catalpol-coated Ti significantly facilitated the formation and remodeling of new bone in osteoporotic rats at 14 days post-implantation. This study outlines a comprehensive and straightforward methodology for the fabrication of biofunctional Ti implants to address osteoporosis.
ACS OmegaChemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍:
ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.