Goldy De Bhowmick*, Maxence Plouviez, Mariza Gomes Reis, Benoit Guieysse, David W. Everett, Michael P. Agnew, Paul Maclean and Caroline Thum*,
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Similarly, the highest total lipid and triacylglycerols (TAGs) recovery from biomass cultivated under nitrogen depletion conditions was found at 100 °C using methanol:chloroform, for <i>C. reinhardtii</i> (total, 550 ± 21; TAG, 205 ± 2 mg/g biomass) and for <i>C. vulgaris</i> (total, 612 ± 29 mg/g; TAG, 253 ± 7 mg/g biomass). ASE with MTBE:methanol:water at 100 °C yielded similar TAG recovery for <i>C. reinhardtii</i> (159 ± 6 mg/g) and <i>C. vulgaris</i> (200 ± 4 mg/g). Thus, MTBE:methanol:water is suggested as an alternative substitute to replace hazardous solvent mixtures for TAGs extraction with a much lower environmental impact. The extracted microalgal TAGs were rich in palmitic (C16:0), stearic (C18:0), oleic (C18:1,9), linoleic (C18:2n6), and α-linolenic (C18:3n3) acids. Under nitrogen depletion conditions, increased palmitic acid (C16:0) recovery up to 2-fold was recorded from the biomasses of <i>C. reinhardtii</i> and <i>C. vulgaris</i>. 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引用次数: 0
摘要
微藻类脂质含有多种脂溶性生物活性化合物,但脂质提取仍是其商业用途的关键限制因素。研究人员采用加速溶剂萃取法(ASE)从标准或氮耗竭条件下生长的蓝藻、螺旋藻和绿藻中提取脂质。在标准生长条件下,使用甲醇:氯仿(2:1)、甲基叔丁基醚(MTBE):甲醇:水和乙醇在 100 °C 下进行 ASE,总脂的回收率最高(分别为 C. reinhardtii、C. vulgaris 和 A. platensis 的 352 ± 30、410 ± 32 和 127 ± 15 mg/g 生物量)。同样,在 100 °C 条件下使用甲醇:氯仿培养的 C. reinhardtii(总脂量,550 ± 21;TAG,205 ± 2 mg/g 生物量)和 C. vulgaris(总脂量,612 ± 29 mg/g;TAG,253 ± 7 mg/g 生物量)的总脂量和三酰甘油(TAG)回收率最高。在 100 °C下使用MTBE:甲醇:水进行 ASE,C. reinhardtii(159 ± 6 mg/g)和 C. vulgaris(200 ± 4 mg/g)的 TAG 回收率相似。因此,MTBE:甲醇:水可替代有害的混合溶剂提取 TAG,对环境的影响更小。提取的微藻 TAG 富含棕榈酸(C16:0)、硬脂酸(C18:0)、油酸(C18:1,9)、亚油酸(C18:2n6)和α-亚麻酸(C18:3n3)。在氮耗竭条件下,C. reinhardtii 和 C. vulgaris 的生物量中棕榈酸(C16:0)的回收率增加了 2 倍。这项研究表明,所采用的提取条件与总脂质和 TAG 回收率之间存在着明显的联系。
Evaluation of Extraction Techniques for Recovery of Microalgal Lipids under Different Growth Conditions
Microalgal lipids contain a wide array of liposoluble bioactive compounds, but lipid extraction remains a critical limitation for their commercial use. An accelerated solvent extraction (ASE) was used to extract lipids from Chlamydomonas reinhardtii, Arthrospira platensis (Spirulina), and Chlorella vulgaris grown under either standard or nitrogen depletion conditions. Under standard growth conditions, ASE using methanol:chloroform (2:1), methyl tert-butyl ether (MTBE):methanol:water, and ethanol at 100 °C resulted in the highest recovery of total lipids (352 ± 30, 410 ± 32, and 127 ± 15 mg/g biomass from C. reinhardtii, C. vulgaris, and A. platensis, respectively). Similarly, the highest total lipid and triacylglycerols (TAGs) recovery from biomass cultivated under nitrogen depletion conditions was found at 100 °C using methanol:chloroform, for C. reinhardtii (total, 550 ± 21; TAG, 205 ± 2 mg/g biomass) and for C. vulgaris (total, 612 ± 29 mg/g; TAG, 253 ± 7 mg/g biomass). ASE with MTBE:methanol:water at 100 °C yielded similar TAG recovery for C. reinhardtii (159 ± 6 mg/g) and C. vulgaris (200 ± 4 mg/g). Thus, MTBE:methanol:water is suggested as an alternative substitute to replace hazardous solvent mixtures for TAGs extraction with a much lower environmental impact. The extracted microalgal TAGs were rich in palmitic (C16:0), stearic (C18:0), oleic (C18:1,9), linoleic (C18:2n6), and α-linolenic (C18:3n3) acids. Under nitrogen depletion conditions, increased palmitic acid (C16:0) recovery up to 2-fold was recorded from the biomasses of C. reinhardtii and C. vulgaris. This study demonstrates a clear linkage between the extraction conditions applied and total lipid and TAG recovery.
ACS OmegaChemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍:
ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.