Circ_0005699 通过靶向 miR-384/ASPH 轴加速 ox-LDL 触发的内皮细胞损伤

IF 3.4 3区 医学 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS Cardiovascular Toxicology Pub Date : 2024-10-01 Epub Date: 2024-07-08 DOI:10.1007/s12012-024-09889-8
Xiaobiao Cao, Jun Yang, Lujun He, Cangcang Liu
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引用次数: 0

摘要

动脉粥样硬化(AS)是一种有多种病因的炎症性疾病。已知多种环状 RNA(circRNA)参与了动脉粥样硬化的发病机制。目的:探讨 circ_0005699 在氧化性低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞(HUVECs)损伤中的功能和机制。Ox-LDL 处理抑制了 HUVECs 的活力、细胞增殖和血管生成能力,并加速了 HUVECs 的细胞凋亡、炎症反应和氧化应激。Circ_0005699在强直性脊柱炎患者和氧化-LDL诱导的HUVECs血清样本中上调。干扰circ_0005699可有效缓解氧化-LDL诱导的HUVEC损伤。此外,miR-384能与circ_0005699结合,miR-384的缺失能逆转circ_0005699缺乏对ox-LDL介导的HUVEC损伤的影响。此外,ASPH 是 miR-384 的直接靶标,ASPH 的强制表达推翻了 miR-384 诱导的对 ox-LDL 诱导的 HUVEC 的影响。重要的是,circ_0005699 通过疏导 miR-384 来调控 ASPH 的表达。对circ_0005699的干扰至少部分是通过充当miR-384的海绵来调节ASPH的表达,从而保护HUVEC免受ox-LDL诱导的损伤。
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Circ_0005699 Expedites ox-LDL-Triggered Endothelial Cell Injury via Targeting miR-384/ASPH Axis.

Atherosclerosis (AS) is an inflammatory disease with multiple causes. Multiple circular RNAs (circRNAs) are known to be involved in the pathogenesis of AS. To explore the function and mechanism of circ_0005699 in oxidative low-density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cells (HUVECs) injury. Ox-LDL treatment restrained HUVECs viability, cell proliferation, and angiogenesis ability, and accelerated HUVECs apoptosis, inflammatory response, and oxidative stress. Circ_0005699 was up-regulated in the serum samples of AS patients and ox-LDL-induced HUVECs. Interference of circ_0005699 effectively rescued ox-LDL-induced injury in HUVECs. Additionally, miR-384 could bind to circ_0005699, and miR-384 depletion inverted the effects of circ_0005699 deficiency on ox-LDL-mediated HUVEC injury. Moreover, ASPH was a direct target of miR-384, and the enforced expression of ASPH overturned miR-384-induced effects on ox-LDL-induced HUVECs. Importantly, circ_0005699 regulated ASPH expression via sponging miR-384. Interference of circ_0005699 protected against ox-LDL-induced injury in HUVECs at least partly by regulating ASPH expression via acting as a miR-384 sponge.

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来源期刊
Cardiovascular Toxicology
Cardiovascular Toxicology 医学-毒理学
CiteScore
6.60
自引率
3.10%
发文量
61
审稿时长
>12 weeks
期刊介绍: Cardiovascular Toxicology is the only journal dedicated to publishing contemporary issues, timely reviews, and experimental and clinical data on toxicological aspects of cardiovascular disease. CT publishes papers that will elucidate the effects, molecular mechanisms, and signaling pathways of environmental toxicants on the cardiovascular system. Also covered are the detrimental effects of new cardiovascular drugs, and cardiovascular effects of non-cardiovascular drugs, anti-cancer chemotherapy, and gene therapy. In addition, Cardiovascular Toxicology reports safety and toxicological data on new cardiovascular and non-cardiovascular drugs.
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