脓疱型苔癣和变异型脓疱病皮损的免疫分型和病毒研究。

IF 1.6 4区 医学 Q3 DERMATOLOGY Journal of Cutaneous Pathology Pub Date : 2024-07-07 DOI:10.1111/cup.14679
Salih Mishlab, Emily Avitan-Hersh, Yaniv Zohar, Moran Szwarcwort-Cohen, Reuven Bergman
{"title":"脓疱型苔癣和变异型脓疱病皮损的免疫分型和病毒研究。","authors":"Salih Mishlab,&nbsp;Emily Avitan-Hersh,&nbsp;Yaniv Zohar,&nbsp;Moran Szwarcwort-Cohen,&nbsp;Reuven Bergman","doi":"10.1111/cup.14679","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>The underlying pathogenesis of pityriasis lichenoides et varioliformis acuta (PLEVA) remains unclear, although immunologic injury and viral etiology have been suggested.</p>\n </section>\n \n <section>\n \n <h3> Objective</h3>\n \n <p>To evaluate and expand the immunophenotype of PLEVA and to search for possible viral pathogens.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Formalin-fixed, paraffin-embedded specimens of 20 patients with PLEVA and 9 patients with common inflammatory dermatoses (ID) were studied for immunophenotyping and for human herpesvirus (HHV) 1 and 2, cytomegalovirus (CMV), HHV-8, parvovirus B19, and Epstein–Barr virus (EBV) immunohistochemistry. The presence of HHV-6, HHV-7, and enteroviruses was assayed molecularly.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The numbers of CD8<sup>+</sup> T cells and T-cell intracellular antigen-1 (TIA-1)<sup>+</sup> cells were statistically significantly higher in PLEVA compared to the ID group. Immunohistochemistry for human HHV-1 and HHV-2, CMV and HHV-8, parvovirus B19, and in situ hybridization for EBV were all negative. There was molecular evidence for HHV-7 in only one PLEVA case (5%). Molecular studies for HHV-6 and enterovirus involvement were negative in all the PLEVA specimens.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The predominant T-cell infiltrate in PLEVA is dominated by CD8<sup>+</sup> cells, and by increased numbers of TIA1<sup>+</sup> cells, which may indicate a cytotoxic T-cell damage to the epidermis. Viral presence was not detected.</p>\n </section>\n </div>","PeriodicalId":15407,"journal":{"name":"Journal of Cutaneous Pathology","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunophenotyping and viral studies in pityriasis lichenoides et varioliformis acuta lesions\",\"authors\":\"Salih Mishlab,&nbsp;Emily Avitan-Hersh,&nbsp;Yaniv Zohar,&nbsp;Moran Szwarcwort-Cohen,&nbsp;Reuven Bergman\",\"doi\":\"10.1111/cup.14679\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>The underlying pathogenesis of pityriasis lichenoides et varioliformis acuta (PLEVA) remains unclear, although immunologic injury and viral etiology have been suggested.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>To evaluate and expand the immunophenotype of PLEVA and to search for possible viral pathogens.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Formalin-fixed, paraffin-embedded specimens of 20 patients with PLEVA and 9 patients with common inflammatory dermatoses (ID) were studied for immunophenotyping and for human herpesvirus (HHV) 1 and 2, cytomegalovirus (CMV), HHV-8, parvovirus B19, and Epstein–Barr virus (EBV) immunohistochemistry. The presence of HHV-6, HHV-7, and enteroviruses was assayed molecularly.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The numbers of CD8<sup>+</sup> T cells and T-cell intracellular antigen-1 (TIA-1)<sup>+</sup> cells were statistically significantly higher in PLEVA compared to the ID group. Immunohistochemistry for human HHV-1 and HHV-2, CMV and HHV-8, parvovirus B19, and in situ hybridization for EBV were all negative. There was molecular evidence for HHV-7 in only one PLEVA case (5%). Molecular studies for HHV-6 and enterovirus involvement were negative in all the PLEVA specimens.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>The predominant T-cell infiltrate in PLEVA is dominated by CD8<sup>+</sup> cells, and by increased numbers of TIA1<sup>+</sup> cells, which may indicate a cytotoxic T-cell damage to the epidermis. Viral presence was not detected.</p>\\n </section>\\n </div>\",\"PeriodicalId\":15407,\"journal\":{\"name\":\"Journal of Cutaneous Pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cutaneous Pathology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/cup.14679\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cutaneous Pathology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cup.14679","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:尽管免疫损伤和病毒病因已被提出,但脓疱性苔藓和变异性苔藓(PLEVA)的潜在发病机制仍不清楚:评估并扩展 PLEVA 的免疫表型,寻找可能的病毒病原体:方法:对20例PLEVA患者和9例常见炎症性皮肤病(ID)患者的福尔马林固定、石蜡包埋标本进行免疫分型和人类疱疹病毒(HHV)1和2、巨细胞病毒(CMV)、HHV-8、副病毒B19和爱泼斯坦-巴尔病毒(EBV)免疫组化研究。分子检测了 HHV-6、HHV-7 和肠道病毒的存在:结果:与 ID 组相比,PLEVA 组 CD8+ T 细胞和 T 细胞胞内抗原-1 (TIA-1)+ 细胞的数量在统计学上明显增加。人类HHV-1和HHV-2、CMV和HHV-8、副病毒B19的免疫组化以及EB病毒的原位杂交均为阴性。只有一个 PLEVA 病例(5%)有 HHV-7 的分子证据。在所有 PLEVA 标本中,HHV-6 和肠道病毒的分子研究均为阴性:结论:PLEVA 中主要的 T 细胞浸润以 CD8+ 细胞为主,TIA1+ 细胞数量增加,这可能表明表皮受到了细胞毒性 T 细胞的损伤。未检测到病毒的存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Immunophenotyping and viral studies in pityriasis lichenoides et varioliformis acuta lesions

Background

The underlying pathogenesis of pityriasis lichenoides et varioliformis acuta (PLEVA) remains unclear, although immunologic injury and viral etiology have been suggested.

Objective

To evaluate and expand the immunophenotype of PLEVA and to search for possible viral pathogens.

Methods

Formalin-fixed, paraffin-embedded specimens of 20 patients with PLEVA and 9 patients with common inflammatory dermatoses (ID) were studied for immunophenotyping and for human herpesvirus (HHV) 1 and 2, cytomegalovirus (CMV), HHV-8, parvovirus B19, and Epstein–Barr virus (EBV) immunohistochemistry. The presence of HHV-6, HHV-7, and enteroviruses was assayed molecularly.

Results

The numbers of CD8+ T cells and T-cell intracellular antigen-1 (TIA-1)+ cells were statistically significantly higher in PLEVA compared to the ID group. Immunohistochemistry for human HHV-1 and HHV-2, CMV and HHV-8, parvovirus B19, and in situ hybridization for EBV were all negative. There was molecular evidence for HHV-7 in only one PLEVA case (5%). Molecular studies for HHV-6 and enterovirus involvement were negative in all the PLEVA specimens.

Conclusions

The predominant T-cell infiltrate in PLEVA is dominated by CD8+ cells, and by increased numbers of TIA1+ cells, which may indicate a cytotoxic T-cell damage to the epidermis. Viral presence was not detected.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.20
自引率
5.90%
发文量
174
审稿时长
3-8 weeks
期刊介绍: Journal of Cutaneous Pathology publishes manuscripts broadly relevant to diseases of the skin and mucosae, with the aims of advancing scientific knowledge regarding dermatopathology and enhancing the communication between clinical practitioners and research scientists. Original scientific manuscripts on diagnostic and experimental cutaneous pathology are especially desirable. Timely, pertinent review articles also will be given high priority. Manuscripts based on light, fluorescence, and electron microscopy, histochemistry, immunology, molecular biology, and genetics, as well as allied sciences, are all welcome, provided their principal focus is on cutaneous pathology. Publication time will be kept as short as possible, ensuring that articles will be quickly available to all interested in this speciality.
期刊最新文献
A Legacy of Life and Science: Personal Reflections on the TITAN. Gene Expression Patterns in a Congenital Neurocristic Hamartoma With Multiple Proliferative Nodules. Histopathologic Comparisons of Discoid Lupus Erythematosus and Folliculotropic Mycosis Fungoides in a Series of 43 Cases. Issue Information Kaposi Sarcoma in the Context of Post-Modified Radical Mastectomy: A New Case Report and Brief Review.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1