{"title":"脓疱型苔癣和变异型脓疱病皮损的免疫分型和病毒研究。","authors":"Salih Mishlab, Emily Avitan-Hersh, Yaniv Zohar, Moran Szwarcwort-Cohen, Reuven Bergman","doi":"10.1111/cup.14679","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>The underlying pathogenesis of pityriasis lichenoides et varioliformis acuta (PLEVA) remains unclear, although immunologic injury and viral etiology have been suggested.</p>\n </section>\n \n <section>\n \n <h3> Objective</h3>\n \n <p>To evaluate and expand the immunophenotype of PLEVA and to search for possible viral pathogens.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Formalin-fixed, paraffin-embedded specimens of 20 patients with PLEVA and 9 patients with common inflammatory dermatoses (ID) were studied for immunophenotyping and for human herpesvirus (HHV) 1 and 2, cytomegalovirus (CMV), HHV-8, parvovirus B19, and Epstein–Barr virus (EBV) immunohistochemistry. The presence of HHV-6, HHV-7, and enteroviruses was assayed molecularly.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The numbers of CD8<sup>+</sup> T cells and T-cell intracellular antigen-1 (TIA-1)<sup>+</sup> cells were statistically significantly higher in PLEVA compared to the ID group. Immunohistochemistry for human HHV-1 and HHV-2, CMV and HHV-8, parvovirus B19, and in situ hybridization for EBV were all negative. There was molecular evidence for HHV-7 in only one PLEVA case (5%). Molecular studies for HHV-6 and enterovirus involvement were negative in all the PLEVA specimens.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The predominant T-cell infiltrate in PLEVA is dominated by CD8<sup>+</sup> cells, and by increased numbers of TIA1<sup>+</sup> cells, which may indicate a cytotoxic T-cell damage to the epidermis. Viral presence was not detected.</p>\n </section>\n </div>","PeriodicalId":15407,"journal":{"name":"Journal of Cutaneous Pathology","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunophenotyping and viral studies in pityriasis lichenoides et varioliformis acuta lesions\",\"authors\":\"Salih Mishlab, Emily Avitan-Hersh, Yaniv Zohar, Moran Szwarcwort-Cohen, Reuven Bergman\",\"doi\":\"10.1111/cup.14679\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>The underlying pathogenesis of pityriasis lichenoides et varioliformis acuta (PLEVA) remains unclear, although immunologic injury and viral etiology have been suggested.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>To evaluate and expand the immunophenotype of PLEVA and to search for possible viral pathogens.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Formalin-fixed, paraffin-embedded specimens of 20 patients with PLEVA and 9 patients with common inflammatory dermatoses (ID) were studied for immunophenotyping and for human herpesvirus (HHV) 1 and 2, cytomegalovirus (CMV), HHV-8, parvovirus B19, and Epstein–Barr virus (EBV) immunohistochemistry. The presence of HHV-6, HHV-7, and enteroviruses was assayed molecularly.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The numbers of CD8<sup>+</sup> T cells and T-cell intracellular antigen-1 (TIA-1)<sup>+</sup> cells were statistically significantly higher in PLEVA compared to the ID group. Immunohistochemistry for human HHV-1 and HHV-2, CMV and HHV-8, parvovirus B19, and in situ hybridization for EBV were all negative. There was molecular evidence for HHV-7 in only one PLEVA case (5%). Molecular studies for HHV-6 and enterovirus involvement were negative in all the PLEVA specimens.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>The predominant T-cell infiltrate in PLEVA is dominated by CD8<sup>+</sup> cells, and by increased numbers of TIA1<sup>+</sup> cells, which may indicate a cytotoxic T-cell damage to the epidermis. Viral presence was not detected.</p>\\n </section>\\n </div>\",\"PeriodicalId\":15407,\"journal\":{\"name\":\"Journal of Cutaneous Pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cutaneous Pathology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/cup.14679\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cutaneous Pathology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cup.14679","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景:尽管免疫损伤和病毒病因已被提出,但脓疱性苔藓和变异性苔藓(PLEVA)的潜在发病机制仍不清楚:评估并扩展 PLEVA 的免疫表型,寻找可能的病毒病原体:方法:对20例PLEVA患者和9例常见炎症性皮肤病(ID)患者的福尔马林固定、石蜡包埋标本进行免疫分型和人类疱疹病毒(HHV)1和2、巨细胞病毒(CMV)、HHV-8、副病毒B19和爱泼斯坦-巴尔病毒(EBV)免疫组化研究。分子检测了 HHV-6、HHV-7 和肠道病毒的存在:结果:与 ID 组相比,PLEVA 组 CD8+ T 细胞和 T 细胞胞内抗原-1 (TIA-1)+ 细胞的数量在统计学上明显增加。人类HHV-1和HHV-2、CMV和HHV-8、副病毒B19的免疫组化以及EB病毒的原位杂交均为阴性。只有一个 PLEVA 病例(5%)有 HHV-7 的分子证据。在所有 PLEVA 标本中,HHV-6 和肠道病毒的分子研究均为阴性:结论:PLEVA 中主要的 T 细胞浸润以 CD8+ 细胞为主,TIA1+ 细胞数量增加,这可能表明表皮受到了细胞毒性 T 细胞的损伤。未检测到病毒的存在。
Immunophenotyping and viral studies in pityriasis lichenoides et varioliformis acuta lesions
Background
The underlying pathogenesis of pityriasis lichenoides et varioliformis acuta (PLEVA) remains unclear, although immunologic injury and viral etiology have been suggested.
Objective
To evaluate and expand the immunophenotype of PLEVA and to search for possible viral pathogens.
Methods
Formalin-fixed, paraffin-embedded specimens of 20 patients with PLEVA and 9 patients with common inflammatory dermatoses (ID) were studied for immunophenotyping and for human herpesvirus (HHV) 1 and 2, cytomegalovirus (CMV), HHV-8, parvovirus B19, and Epstein–Barr virus (EBV) immunohistochemistry. The presence of HHV-6, HHV-7, and enteroviruses was assayed molecularly.
Results
The numbers of CD8+ T cells and T-cell intracellular antigen-1 (TIA-1)+ cells were statistically significantly higher in PLEVA compared to the ID group. Immunohistochemistry for human HHV-1 and HHV-2, CMV and HHV-8, parvovirus B19, and in situ hybridization for EBV were all negative. There was molecular evidence for HHV-7 in only one PLEVA case (5%). Molecular studies for HHV-6 and enterovirus involvement were negative in all the PLEVA specimens.
Conclusions
The predominant T-cell infiltrate in PLEVA is dominated by CD8+ cells, and by increased numbers of TIA1+ cells, which may indicate a cytotoxic T-cell damage to the epidermis. Viral presence was not detected.
期刊介绍:
Journal of Cutaneous Pathology publishes manuscripts broadly relevant to diseases of the skin and mucosae, with the aims of advancing scientific knowledge regarding dermatopathology and enhancing the communication between clinical practitioners and research scientists. Original scientific manuscripts on diagnostic and experimental cutaneous pathology are especially desirable. Timely, pertinent review articles also will be given high priority. Manuscripts based on light, fluorescence, and electron microscopy, histochemistry, immunology, molecular biology, and genetics, as well as allied sciences, are all welcome, provided their principal focus is on cutaneous pathology. Publication time will be kept as short as possible, ensuring that articles will be quickly available to all interested in this speciality.