增大聚合体尺寸可减少嵌入水凝胶的湿地微生物对单细胞有机碳的吸收。

IF 5.1 Q1 ECOLOGY ISME communications Pub Date : 2024-06-20 eCollection Date: 2024-01-01 DOI:10.1093/ismeco/ycae086
Juliet T Johnston, Bao Nguyen Quoc, Britt Abrahamson, Pieter Candry, Christina Ramon, Kevin J Cash, Sam C Saccomano, Ty J Samo, Congwang Ye, Peter K Weber, Mari-Karoliina Henriikka Winkler, Xavier Mayali
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引用次数: 0

摘要

沉积物中有机碳的微生物降解受到氧气和生长基质供应的影响。为了更好地了解颗粒大小和氧化还原带如何影响微生物有机碳的吸收,有必要采用保持空间信息的技术来量化微观尺度上的元素循环。在这项研究中,我们制作了不同直径(100、250 和 500 μm)的水凝胶微球,并在其中接种了从淡水湿地分离出来的好氧异养细菌(黄杆菌属),在第二次实验中接种了从城市湖沼湿地分离出来的微生物群落。水凝胶包埋的微生物种群与 13C 标记的底物一起培养,通过纳米吸附质谱(nanoSIMS)量化有机碳融入生物量的情况。此外,发光纳米传感器还能对微球内部的氧气浓度进行明确的空间测量。实验数据随后被纳入反应传输模型,以预测长期稳态条件。与较大的颗粒(250 微米和 500 微米)相比,较小(100 微米)的颗粒在单位体积内表现出最高的微生物细胞特异性生长,但在表面附近也表现出更高的绝对活性。实验结果和计算模型表明,有机碳的供应量不足以形成陡峭的氧气梯度,因此,所有尺寸的颗粒都保持了良好的供氧条件。我们的研究为今后利用同位素标记的底物来量化聚集体中微生物的生长情况,从而调查其空间依赖性活动的研究提供了一个基础框架。
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Increasing aggregate size reduces single-cell organic carbon incorporation by hydrogel-embedded wetland microbes.

Microbial degradation of organic carbon in sediments is impacted by the availability of oxygen and substrates for growth. To better understand how particle size and redox zonation impact microbial organic carbon incorporation, techniques that maintain spatial information are necessary to quantify elemental cycling at the microscale. In this study, we produced hydrogel microspheres of various diameters (100, 250, and 500 μm) and inoculated them with an aerobic heterotrophic bacterium isolated from a freshwater wetland (Flavobacterium sp.), and in a second experiment with a microbial community from an urban lacustrine wetland. The hydrogel-embedded microbial populations were incubated with 13C-labeled substrates to quantify organic carbon incorporation into biomass via nanoSIMS. Additionally, luminescent nanosensors enabled spatially explicit measurements of oxygen concentrations inside the microspheres. The experimental data were then incorporated into a reactive-transport model to project long-term steady-state conditions. Smaller (100 μm) particles exhibited the highest microbial cell-specific growth per volume, but also showed higher absolute activity near the surface compared to the larger particles (250 and 500 μm). The experimental results and computational models demonstrate that organic carbon availability was not high enough to allow steep oxygen gradients and as a result, all particle sizes remained well-oxygenated. Our study provides a foundational framework for future studies investigating spatially dependent microbial activity in aggregates using isotopically labeled substrates to quantify growth.

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