Developing the ‘omic toolkit of comparative physiologists

Typical ‘omic analyses reduce complex biological systems to simple lists of supposedly independent variables, failing to account for changes in the wider transcriptional landscape. In this commentary, we discuss the utility of network approaches for incorporating this wider context into the study of physiological phenomena. We highlight opportunities to build on traditional network tools by utilising cutting-edge techniques to account for higher order interactions (i.e. beyond pairwise associations) within datasets, allowing for more accurate models of complex ‘omic systems. Finally, we show examples of previous works utilising network approaches to gain additional insight into their organisms of interest. As ‘omics grow in both their popularity and breadth of application, so does the requirement for flexible analytical tools capable of interpreting and synthesising complex datasets.