将元基因组下一代测序作为 HIV 阴性患者滑膜真菌病的有效诊断工具。

IF 3.6 3区生物学 Q2 MYCOLOGY Mycopathologia Pub Date : 2024-07-10 DOI:10.1007/s11046-024-00866-x

Li Jiang, Tian-Wei Liang, Najwa Al-Odaini, Yuan Hu, Minli Huang, Lili Wei, Xiu-Ying Li, Kai-Su Pan, Dong-Yan Zheng, Zhi-Wen Jiang, Gao Wei, Cun-Wei Cao

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引用次数: 0

摘要

在艾滋病毒阴性患者中诊断马内菲氏塔拉罗米菌感染仍然具有挑战性。目前迫切需要快速便捷的方法来诊断这种复杂的疾病。本研究旨在通过比较 mNGS 和传统微生物培养法，评估元基因组新一代测序（mNGS）对非 HIV 感染者滑轮霉菌病的诊断效率。共有来自 57 名患者的 66 份样本通过 mNGS 和微生物培养进行了分析。ROC 曲线显示，mNGS 的灵敏度为 97.22%，高于微生物培养的灵敏度（61.11%）。建议将呼吸道、感染性皮肤病变和淋巴结样本作为通过 mNGS 检测滑液菌病的常规样本。此外，与微生物培养相比，mNGS 能大大缩短诊断时间。总之，我们的研究表明，mNGS 是一种很有前途的工具，可用于快速、准确地检测 HIV 阴性滑液菌病患者的病原体。

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Metagenomic Next-Generation Sequencing as an Effective Diagnostic Tool for Talaromycosis in HIV-Negative Patients.

The diagnosis of Talaromyces marneffei infection in HIV-negative patients remains challenging. There is an urgent need for rapid and convenient methods to diagnose this complicated disease. The aim of this study was to evaluate the diagnostic efficiency of metagenomic next-generation sequencing (mNGS) for talaromycosis in non-HIV-infected patients by comparing mNGS with traditional microbial culture. In total, 66 samples from 57 patients were analyzed via both mNGS and microbial culture. The ROC curve showed a sensitivity for mNGS of 97.22%, which was greater than that of microbial culture (61.11%). Samples from the respiratory tract, infectious skin lesions, and lymph nodes are recommended as routine samples for talaromycosis detection via mNGS. Furthermore, mNGS significantly reduced the diagnostic time compared to microbial culture. Overall, our study demonstrated that mNGS is a promising tool for rapid and accurate pathogenic detection in HIV-negative patients with talaromycosis.

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来源期刊

Mycopathologia 生物-真菌学

CiteScore

6.80

自引率

3.60%

发文量

审稿时长

3 months

期刊介绍： Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.