介质酸度对水溶液中金纳米粒子和牛血清白蛋白相互作用影响的光学研究

IF 1.1 4区物理与天体物理 Q3 PHYSICS, MULTIDISCIPLINARY Physics of Wave Phenomena Pub Date : 2024-07-08 DOI:10.3103/S1541308X24700213

E. A. Molkova, V. I. Pustovoi, I. V. Baimler, A. V. Simakin, D. E. Burmistrov, I. V. Gorudko, S. V. Gudkov

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摘要

摘要通过吸光度光谱法、荧光光谱法和动态光散射法（DLS）研究了溶液酸度对溶液中牛血清白蛋白（BSA）分子和金纳米粒子之间相互作用的影响。结果表明，pH 值对有 BSA 和无 BSA 的金纳米粒子的聚集-解聚过程以及蛋白质溶液的变性都有影响。研究还表明，在 pH 值为 2.0-4.0 的酸性环境中，BSA 分子可以稳定金纳米粒子。获得的数据对生理学家研究纳米粒子对人体不同生物介质的影响很有帮助。

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Optical Study of the Influence of the Medium Acidity on the Interaction between Gold Nanoparticles and Bovine Serum Albumin in Aqueous Solution

The influence of the solution acidity on the interaction between bovine serum albumin (BSA) molecules and gold nanoparticles in solutions has been investigated by absorbance spectroscopy, fluorescence spectroscopy, and dynamic light scattering (DLS). The influence of pH on the processes of aggregation–disaggregation of gold nanoparticles with BSA and without it and on the denaturation of protein solution is demonstrated. It is also shown that BSA molecules can stabilize gold nanoparticles at acidic pH values of 2.0–4.0. The data obtained can be useful for physiologists studying the influence of nanoparticles on different biological media of the body.

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来源期刊

Physics of Wave Phenomena PHYSICS, MULTIDISCIPLINARY-

CiteScore

2.50

自引率

21.40%

发文量

审稿时长

>12 weeks

期刊介绍： Physics of Wave Phenomena publishes original contributions in general and nonlinear wave theory, original experimental results in optics, acoustics and radiophysics. The fields of physics represented in this journal include nonlinear optics, acoustics, and radiophysics; nonlinear effects of any nature including nonlinear dynamics and chaos; phase transitions including light- and sound-induced; laser physics; optical and other spectroscopies; new instruments, methods, and measurements of wave and oscillatory processes; remote sensing of waves in natural media; wave interactions in biophysics, econophysics and other cross-disciplinary areas.