利用磁化功能化柔性微磁体阵列研究聚苯乙烯微珠在集成微流体系统中的聚焦和分离问题

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Microfluidics and Nanofluidics Pub Date : 2024-07-09 DOI:10.1007/s10404-024-02749-5
Shuang Chen, Jiajia Sun, Zongqian Shi, Xiaofeng Liu, Yuxin Ma, Ruohan Li, Shumin Xin, Nan Wang, Xiaoling Li, Kai Wu
{"title":"利用磁化功能化柔性微磁体阵列研究聚苯乙烯微珠在集成微流体系统中的聚焦和分离问题","authors":"Shuang Chen,&nbsp;Jiajia Sun,&nbsp;Zongqian Shi,&nbsp;Xiaofeng Liu,&nbsp;Yuxin Ma,&nbsp;Ruohan Li,&nbsp;Shumin Xin,&nbsp;Nan Wang,&nbsp;Xiaoling Li,&nbsp;Kai Wu","doi":"10.1007/s10404-024-02749-5","DOIUrl":null,"url":null,"abstract":"<div><p>Focusing and separation of cells by microfluidic techniques are significant steps in many applications, such as single-cell analysis and disease diagnosis. Among the microfluidic techniques, passive magnetophoresis, as a label-free manner, can manipulate samples by means of magnetic field. Nowadays, most magnetic fields are generated by permanent magnets and electromagnets with large size. However, it is difficult to assemble a magnetic array using permanent magnets or electromagnets to optimize the field distribution. To produce a flexible magnetic field, a micro-magnet made by NdFeB powder and polydimethyl siloxane is proposed in this paper, and those magnetized micro-magnets are arranged into different arrays according to the arrangements of their magnetization directions. Meanwhile, a microfluidic chip containing magnetized micro-magnet arrays is designed for focusing and separating polystyrene microbeads with different diameters. The focusing and separation behaviors of microbeads in the designed microfluidic system are numerical and experimental investigated. In addition, the effects of flow rate and the arrangement of the magnetic micro-magnet array on microbead focusing and separation are discussed. Finally, a multistage microfluidic chip is designed to successfully isolate 5 μm-diameter, 10 μm-diameter, and 15 μm-diameter microbeads from their mixture at a flow rate of 240 μL/min with high purity.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation on the focusing and separation of polystyrene microbeads in an integrated microfluidic system using magnetized functionalized flexible micro-magnet arrays\",\"authors\":\"Shuang Chen,&nbsp;Jiajia Sun,&nbsp;Zongqian Shi,&nbsp;Xiaofeng Liu,&nbsp;Yuxin Ma,&nbsp;Ruohan Li,&nbsp;Shumin Xin,&nbsp;Nan Wang,&nbsp;Xiaoling Li,&nbsp;Kai Wu\",\"doi\":\"10.1007/s10404-024-02749-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Focusing and separation of cells by microfluidic techniques are significant steps in many applications, such as single-cell analysis and disease diagnosis. Among the microfluidic techniques, passive magnetophoresis, as a label-free manner, can manipulate samples by means of magnetic field. Nowadays, most magnetic fields are generated by permanent magnets and electromagnets with large size. However, it is difficult to assemble a magnetic array using permanent magnets or electromagnets to optimize the field distribution. To produce a flexible magnetic field, a micro-magnet made by NdFeB powder and polydimethyl siloxane is proposed in this paper, and those magnetized micro-magnets are arranged into different arrays according to the arrangements of their magnetization directions. Meanwhile, a microfluidic chip containing magnetized micro-magnet arrays is designed for focusing and separating polystyrene microbeads with different diameters. The focusing and separation behaviors of microbeads in the designed microfluidic system are numerical and experimental investigated. In addition, the effects of flow rate and the arrangement of the magnetic micro-magnet array on microbead focusing and separation are discussed. Finally, a multistage microfluidic chip is designed to successfully isolate 5 μm-diameter, 10 μm-diameter, and 15 μm-diameter microbeads from their mixture at a flow rate of 240 μL/min with high purity.</p></div>\",\"PeriodicalId\":706,\"journal\":{\"name\":\"Microfluidics and Nanofluidics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-07-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microfluidics and Nanofluidics\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10404-024-02749-5\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INSTRUMENTS & INSTRUMENTATION\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microfluidics and Nanofluidics","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10404-024-02749-5","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INSTRUMENTS & INSTRUMENTATION","Score":null,"Total":0}
引用次数: 0

摘要

利用微流体技术聚焦和分离细胞是单细胞分析和疾病诊断等许多应用中的重要步骤。在微流体技术中,无源磁泳作为一种无标记的方式,可以通过磁场操纵样品。目前,磁场大多由体积较大的永久磁铁和电磁铁产生。然而,使用永久磁铁或电磁铁组装磁阵列以优化磁场分布却很困难。为了产生柔性磁场,本文提出了一种由钕铁硼粉末和聚二甲基硅氧烷制成的微型磁体,并根据磁化方向的排列将这些磁化的微型磁体排列成不同的阵列。同时,设计了一种包含磁化微磁体阵列的微流控芯片,用于聚焦和分离不同直径的聚苯乙烯微珠。通过数值和实验研究了微珠在所设计的微流控系统中的聚焦和分离行为。此外,还讨论了流速和磁性微磁体阵列排列对微珠聚焦和分离的影响。最后,设计了一种多级微流控芯片,可在 240 μL/min 的流速下成功地从混合物中分离出直径为 5 μm、10 μm 和 15 μm 的微珠,且分离纯度很高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Investigation on the focusing and separation of polystyrene microbeads in an integrated microfluidic system using magnetized functionalized flexible micro-magnet arrays

Focusing and separation of cells by microfluidic techniques are significant steps in many applications, such as single-cell analysis and disease diagnosis. Among the microfluidic techniques, passive magnetophoresis, as a label-free manner, can manipulate samples by means of magnetic field. Nowadays, most magnetic fields are generated by permanent magnets and electromagnets with large size. However, it is difficult to assemble a magnetic array using permanent magnets or electromagnets to optimize the field distribution. To produce a flexible magnetic field, a micro-magnet made by NdFeB powder and polydimethyl siloxane is proposed in this paper, and those magnetized micro-magnets are arranged into different arrays according to the arrangements of their magnetization directions. Meanwhile, a microfluidic chip containing magnetized micro-magnet arrays is designed for focusing and separating polystyrene microbeads with different diameters. The focusing and separation behaviors of microbeads in the designed microfluidic system are numerical and experimental investigated. In addition, the effects of flow rate and the arrangement of the magnetic micro-magnet array on microbead focusing and separation are discussed. Finally, a multistage microfluidic chip is designed to successfully isolate 5 μm-diameter, 10 μm-diameter, and 15 μm-diameter microbeads from their mixture at a flow rate of 240 μL/min with high purity.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
期刊最新文献
Visualizing conformance control mechanisms in high-temperature reservoirs: a microfluidic analysis of Pickering emulsified gel systems Exploring fluid flow in microchannels with branching and variable constrictions Variance-reduction kinetic simulation for characterization of surface and corner effects in low-speed rarefied gas flows through long micro-ducts Dynamic response of a weakly ionized fluid in a vibrating Riga channel exposed to intense electromagnetic rotation Physiological hypoxia promotes cancer cell migration and attenuates angiogenesis in co-culture using a microfluidic device
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1