EquCab_Finn:家马芬马的新参考基因组组装。

IF 1.8 3区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal genetics Pub Date : 2024-07-10 DOI:10.1111/age.13463
Kisun Pokharel, Melak Weldenegodguad, Tiina Reilas, Juha Kantanen
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引用次数: 0

摘要

芬兰马是芬兰本土的国家马种,与北欧和亚洲的马有亲缘关系。它在农业、森林作业、运输以及作为战马方面具有重要的历史意义。芬兰马在种马目录中有四个繁殖区,目前正在进行保护和特征描述工作。我们使用 PacBio 和 Omni-C 数据对来自工作马区的一匹芬霍马母马的基因组进行了测序和注释。该基因组可以补充现有的纯血马参考基因组(EquCab 3.0),促进对欧亚大陆北部马匹的遗传研究。我们组装了 2.4 Gb 的基因组,N50 支架长度为 83.8 Mb,通过基因组注释共获得 19 748 个蛋白质编码基因,其中 1200 个为芬兰马特异基因。基因组注释共得到 19 748 个蛋白质编码基因,其中 1200 个是芬兰马特有的。该基因组与当前的马参考基因组具有很高的质量和同源性。我们对五个感兴趣的基因进行了人工标注,并将最终组装结果存入欧洲核苷酸档案,登录号为 PRJEB71364。PRJEB71364。
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EquCab_Finn: A new reference genome assembly for the domestic horse, Finnhorse

Finnhorse is Finland's native and national horse breed and it has genetic affinities to northern European and Asian horses. It has historical importance for agriculture, forest work and transport and as a war horse. Finnhorse has four breeding sections in the studbook and is under conservation and characterisation efforts. We sequenced and annotated the genome of a Finnhorse mare from the working horse section using PacBio and Omni-C data. This genome can complement the existing Thoroughbred reference genome (EquCab 3.0) and facilitate genetic studies of horses from northern Eurasia. We assembled 2.4 Gb of the genome with an N50 scaffold length of 83.8 Mb and the genome annotation resulted in a total of 19 748 protein coding genes of which 1200 were Finnhorse specific. The assembly has high quality and synteny with the current horse reference genome. We manually curated five genes of interest and deposited the final assembly in the European Nucleotide Archive under the accession no. PRJEB71364.

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来源期刊
Animal genetics
Animal genetics 生物-奶制品与动物科学
CiteScore
4.60
自引率
4.20%
发文量
115
审稿时长
5 months
期刊介绍: Animal Genetics reports frontline research on immunogenetics, molecular genetics and functional genomics of economically important and domesticated animals. Publications include the study of variability at gene and protein levels, mapping of genes, traits and QTLs, associations between genes and traits, genetic diversity, and characterization of gene or protein expression and control related to phenotypic or genetic variation. The journal publishes full-length articles, short communications and brief notes, as well as commissioned and submitted mini-reviews on issues of interest to Animal Genetics readers.
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