Oliver Sánchez-Rodríguez, Erika G Palomares-Resendiz, Efrén Díaz-Aparicio, Beatriz Arellano-Reynoso, Luary C Martínez-Chavarría, Guadalupe Martínez-Serrano, Rigoberto Hernández-Castro
{"title":"流产绵羊阴道拭子样本中燃烧性柯西氏菌的分子检测","authors":"Oliver Sánchez-Rodríguez, Erika G Palomares-Resendiz, Efrén Díaz-Aparicio, Beatriz Arellano-Reynoso, Luary C Martínez-Chavarría, Guadalupe Martínez-Serrano, Rigoberto Hernández-Castro","doi":"10.1089/vbz.2023.0153","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Background:</i></b> <i>Coxiella burnetii</i>, an obligate intracellular bacterium, is the etiological agent of Q fever in humans and one of the causes of abortion in small ruminants. Although coxiellosis is considered an exotic disease, there are a few reports in Mexico. <b><i>Methods:</i></b> The objective of this work was to determine the presence of <i>C</i>. <i>burnetii</i> DNA in vaginal samples from sheep that presented abortion and ram semen. A total of 180 vaginal exudate samples and 20 semen samples were obtained from five Central and Southern States of Mexico. Total DNA was extracted from vaginal swabs and <i>C</i>. <i>burnetii</i> was identified by PCR amplification and sequencing of the IS<i>1111</i> insertion sequence. <b><i>Results and Conclusion:</i></b> In total, 110 (110/180) vaginal samples and 12 (12/20) semen samples were positive for <i>C</i>. <i>burnetii</i>. This is the first report of <i>C</i>. <i>burnetii</i> in sheep that aborted and in ram semen in Mexico.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular Detection of <i>Coxiella burnetii</i> in Vaginal Swab Samples from Sheep That Aborted.\",\"authors\":\"Oliver Sánchez-Rodríguez, Erika G Palomares-Resendiz, Efrén Díaz-Aparicio, Beatriz Arellano-Reynoso, Luary C Martínez-Chavarría, Guadalupe Martínez-Serrano, Rigoberto Hernández-Castro\",\"doi\":\"10.1089/vbz.2023.0153\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b><i>Background:</i></b> <i>Coxiella burnetii</i>, an obligate intracellular bacterium, is the etiological agent of Q fever in humans and one of the causes of abortion in small ruminants. Although coxiellosis is considered an exotic disease, there are a few reports in Mexico. <b><i>Methods:</i></b> The objective of this work was to determine the presence of <i>C</i>. <i>burnetii</i> DNA in vaginal samples from sheep that presented abortion and ram semen. A total of 180 vaginal exudate samples and 20 semen samples were obtained from five Central and Southern States of Mexico. Total DNA was extracted from vaginal swabs and <i>C</i>. <i>burnetii</i> was identified by PCR amplification and sequencing of the IS<i>1111</i> insertion sequence. <b><i>Results and Conclusion:</i></b> In total, 110 (110/180) vaginal samples and 12 (12/20) semen samples were positive for <i>C</i>. <i>burnetii</i>. This is the first report of <i>C</i>. <i>burnetii</i> in sheep that aborted and in ram semen in Mexico.</p>\",\"PeriodicalId\":23683,\"journal\":{\"name\":\"Vector borne and zoonotic diseases\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vector borne and zoonotic diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1089/vbz.2023.0153\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vector borne and zoonotic diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1089/vbz.2023.0153","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Molecular Detection of Coxiella burnetii in Vaginal Swab Samples from Sheep That Aborted.
Background:Coxiella burnetii, an obligate intracellular bacterium, is the etiological agent of Q fever in humans and one of the causes of abortion in small ruminants. Although coxiellosis is considered an exotic disease, there are a few reports in Mexico. Methods: The objective of this work was to determine the presence of C. burnetii DNA in vaginal samples from sheep that presented abortion and ram semen. A total of 180 vaginal exudate samples and 20 semen samples were obtained from five Central and Southern States of Mexico. Total DNA was extracted from vaginal swabs and C. burnetii was identified by PCR amplification and sequencing of the IS1111 insertion sequence. Results and Conclusion: In total, 110 (110/180) vaginal samples and 12 (12/20) semen samples were positive for C. burnetii. This is the first report of C. burnetii in sheep that aborted and in ram semen in Mexico.
期刊介绍：
Vector-Borne and Zoonotic Diseases is an authoritative, peer-reviewed journal providing basic and applied research on diseases transmitted to humans by invertebrate vectors or non-human vertebrates. The Journal examines geographic, seasonal, and other risk factors that influence the transmission, diagnosis, management, and prevention of this group of infectious diseases, and identifies global trends that have the potential to result in major epidemics.
Vector-Borne and Zoonotic Diseases coverage includes:
-Ecology
-Entomology
-Epidemiology
-Infectious diseases
-Microbiology
-Parasitology
-Pathology
-Public health
-Tropical medicine
-Wildlife biology
-Bacterial, rickettsial, viral, and parasitic zoonoses
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