转录组比较分析揭示了日本鲭在受到 polyI:C 挑战后卵巢和肝胰腺的不同反应机制

IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2024-07-06 DOI:10.1016/j.cbd.2024.101289
Haifu Wan , Lei Yu , Xiaodong Cui , Shuai Guo , Shumei Mu , Xianjiang Kang
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引用次数: 0

摘要

哺乳动物的卵巢已发展出专门的机制来抵御病原体感染;然而,人们对甲壳类动物卵巢先天性免疫系统的了解仍然有限。为了阐明卵巢应对病毒挑战的防御机制,我们将东方对虾(Macrobrachium nipponense)置于仿病毒dsRNA的双链RNA类似物poly I:C中,并分析了卵巢的转录组特征。同时,为了研究卵巢和肝胰腺的不同反应机制,并全面了解这两种组织的免疫反应,我们还对肝胰腺(一种公认的免疫相关组织)进行了多聚 I:C 挑战后的 RNA 序列分析。结果表明,1368 个基因在卵巢中差异表达,其中 903 个基因上调,465 个基因下调。随后的分析表明,这些差异表达基因(DEGs)包括许多与先天性免疫相关的基因,如C型凝集素、纤维蛋白原相关蛋白(Frep)、Toll样受体和NOD样受体(NLR)基因家族成员,以及酸性磷酸酶、清道夫受体、甲壳素、唐氏综合征细胞粘附分子(Dscam)、血蓝蛋白、脂多糖和β-1,3-葡聚糖结合蛋白(LGBP)。此外,DEGs还包括几个与卵巢发育有关的基因,如sox8、卵黄素、原粒蛋白、细胞周期蛋白依赖性激酶、蜕皮激素受体、frizzled和Fox基因家族成员。在肝胰腺中,共发现了 729 个 DEGs。对这两种组织中的 DEGs 进行比较后发现,只有 91 个基因在两组中是共通的,这凸显了组织对 poly I:C 刺激的显著特异性反应。这项研究旨在加深我们对卵巢应对病原体暴露所采用的免疫保护机制的了解,并为研究甲壳类动物的卵巢生殖免疫奠定基础。
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Comparative transcriptome analysis reveals the different responding mechanisms of ovary and hepatopancreas following polyI:C challenge in Macrobrachium nipponense

The ovary in mammals has developed specialized mechanisms for protection against pathogen infections; however, the understanding of the innate immune system in the ovary of crustaceans is still limited. To elucidate the ovary's defense mechanisms in response to viral challenges, we subjected oriental river prawns (Macrobrachium nipponense) to poly I:C, a double-stranded RNA analog that emulates viral dsRNA, and analyzed the ovary's transcriptome profiles. Concurrently, RNA-seq analysis was performed on the hepatopancreas, a well-recognized immune-related tissue, following poly I:C challenge to investigate the distinct response mechanisms of the ovary and hepatopancreas and to gain a comprehensive understanding of the immune responses in both tissues. The results indicate that 1368 genes are differentially expressed in the ovary, with 903 genes upregulated and 465 genes downregulated. Subsequent analysis reveals that these differentially expressed genes (DEGs) include numerous genes associated with innate immunity, such as members of the C-type lectin, fibrinogen-related protein (Frep), Toll-like receptor, and NOD-like receptor (NLR) gene families, as well as acid phosphatase, scavenger receptor, crustin, Down syndrome cell adhesion molecule (Dscam), hemocyanin, and lipopolysaccharide and beta-1,3-glucan binding protein (LGBP). Furthermore, the DEGs include several genes related to ovary development, such as sox8, vitellogenin, progranulin, cyclin-dependent kinase, ecdysone receptor, frizzled, and members of the Fox gene family. In the hepatopancreas, a total of 729 DEGs were identified. Comparison of the DEGs in both tissues indicates that only 91 genes are common to both groups, highlighting significant tissue-specific responses to poly I:C stimulation. This study aims to enhance our understanding of the immune protective mechanisms employed by the ovary in response to pathogen exposure and establishes a foundation for investigating ovarian reproductive immunity in crustaceans.

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来源期刊
CiteScore
5.10
自引率
3.30%
发文量
69
审稿时长
33 days
期刊介绍: Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.
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