Ping Wei , Shiying Huang , Juan Yang , Mo Zhao , Qiugu Chen , Xiaoyu Deng , Jianping Chen , Yisheng Li
{"title":"UPLC-Q-TOF/MS鉴定和表征麻黄桂枝煎剂中的化学成分及其在大鼠血浆和脑中的代谢物","authors":"Ping Wei , Shiying Huang , Juan Yang , Mo Zhao , Qiugu Chen , Xiaoyu Deng , Jianping Chen , Yisheng Li","doi":"10.1016/j.chmed.2024.01.006","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Mahuang Guizhi Decoction (MGD), an essential herbal pair in traditional Chinese medicine, is able to release cold, fever and asthma, mainly containing alkaloids, flavonoids, phenylpropanoids and amino acids. However, the absorption and distribution of these four category compounds <em>in vivo</em> still remained unclearly.</p></div><div><h3>Methods</h3><p>In our research, we utilized UPLC-Q-TOF-MS technique to identify the constituents within MGD, as well as the prototypes of MGD and their metabolites absorbed in plasma and brain. We further profiled the drug-time curve of prototypes and metabolites of MGD both in plasma and brain.</p></div><div><h3>Results</h3><p>Our results showed that 105 constituents were characterized in MGD. Thirty of them could be absorbed into blood, and ten of them could be distributed into brain. We also discovered eight new bio-transformed metabolites in blood, and a half of which could pass through the blood–brain barrier. In addition, all components detected <em>in vivo</em> could be absorbed and distributed immediately.</p></div><div><h3>Conclusion</h3><p>These findings provide an approachable method to analyze the potential bio-active compounds in MGD and their <em>in vivo</em> behaviors, which could promote the efficacious material basis study of MGD and the security of clinical utilization.</p></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"16 3","pages":"Pages 466-480"},"PeriodicalIF":4.7000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1674638424000479/pdfft?md5=65c0244ee53b36a8b86b7af6cc5fb975&pid=1-s2.0-S1674638424000479-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Identification and characterization of chemical constituents in Mahuang Guizhi Decoction and their metabolites in rat plasma and brain by UPLC-Q-TOF/MS\",\"authors\":\"Ping Wei , Shiying Huang , Juan Yang , Mo Zhao , Qiugu Chen , Xiaoyu Deng , Jianping Chen , Yisheng Li\",\"doi\":\"10.1016/j.chmed.2024.01.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>Mahuang Guizhi Decoction (MGD), an essential herbal pair in traditional Chinese medicine, is able to release cold, fever and asthma, mainly containing alkaloids, flavonoids, phenylpropanoids and amino acids. However, the absorption and distribution of these four category compounds <em>in vivo</em> still remained unclearly.</p></div><div><h3>Methods</h3><p>In our research, we utilized UPLC-Q-TOF-MS technique to identify the constituents within MGD, as well as the prototypes of MGD and their metabolites absorbed in plasma and brain. We further profiled the drug-time curve of prototypes and metabolites of MGD both in plasma and brain.</p></div><div><h3>Results</h3><p>Our results showed that 105 constituents were characterized in MGD. Thirty of them could be absorbed into blood, and ten of them could be distributed into brain. We also discovered eight new bio-transformed metabolites in blood, and a half of which could pass through the blood–brain barrier. In addition, all components detected <em>in vivo</em> could be absorbed and distributed immediately.</p></div><div><h3>Conclusion</h3><p>These findings provide an approachable method to analyze the potential bio-active compounds in MGD and their <em>in vivo</em> behaviors, which could promote the efficacious material basis study of MGD and the security of clinical utilization.</p></div>\",\"PeriodicalId\":9916,\"journal\":{\"name\":\"Chinese Herbal Medicines\",\"volume\":\"16 3\",\"pages\":\"Pages 466-480\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1674638424000479/pdfft?md5=65c0244ee53b36a8b86b7af6cc5fb975&pid=1-s2.0-S1674638424000479-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Herbal Medicines\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1674638424000479\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Herbal Medicines","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1674638424000479","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Identification and characterization of chemical constituents in Mahuang Guizhi Decoction and their metabolites in rat plasma and brain by UPLC-Q-TOF/MS
Objective
Mahuang Guizhi Decoction (MGD), an essential herbal pair in traditional Chinese medicine, is able to release cold, fever and asthma, mainly containing alkaloids, flavonoids, phenylpropanoids and amino acids. However, the absorption and distribution of these four category compounds in vivo still remained unclearly.
Methods
In our research, we utilized UPLC-Q-TOF-MS technique to identify the constituents within MGD, as well as the prototypes of MGD and their metabolites absorbed in plasma and brain. We further profiled the drug-time curve of prototypes and metabolites of MGD both in plasma and brain.
Results
Our results showed that 105 constituents were characterized in MGD. Thirty of them could be absorbed into blood, and ten of them could be distributed into brain. We also discovered eight new bio-transformed metabolites in blood, and a half of which could pass through the blood–brain barrier. In addition, all components detected in vivo could be absorbed and distributed immediately.
Conclusion
These findings provide an approachable method to analyze the potential bio-active compounds in MGD and their in vivo behaviors, which could promote the efficacious material basis study of MGD and the security of clinical utilization.
期刊介绍:
Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included.
CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.