UPLC-Q-TOF/MS鉴定和表征麻黄桂枝煎剂中的化学成分及其在大鼠血浆和脑中的代谢物

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2024-07-01 DOI:10.1016/j.chmed.2024.01.006
Ping Wei , Shiying Huang , Juan Yang , Mo Zhao , Qiugu Chen , Xiaoyu Deng , Jianping Chen , Yisheng Li
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引用次数: 0

摘要

目的 麻黄桂枝汤(MGD)是中药中不可或缺的一副中药,具有解表散寒、清热平喘的功效,主要成分为生物碱、黄酮类、苯丙类和氨基酸。在研究中,我们利用 UPLC-Q-TOF-MS 技术鉴定了味甘草中的成分,以及在血浆和脑中吸收的味甘草原型及其代谢物。我们进一步分析了 MGD 原型和代谢物在血浆和大脑中的药物时间曲线。结果表明,在 MGD 中发现了 105 种成分,其中 30 种可被血液吸收,10 种可进入大脑。我们还在血液中发现了八种新的生物转化代谢物,其中一半可以通过血脑屏障。结论:这些发现为分析 MGD 中潜在的生物活性化合物及其体内行为提供了一种简便易行的方法,可促进 MGD 的有效物质基础研究和临床应用的安全性。
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Identification and characterization of chemical constituents in Mahuang Guizhi Decoction and their metabolites in rat plasma and brain by UPLC-Q-TOF/MS

Objective

Mahuang Guizhi Decoction (MGD), an essential herbal pair in traditional Chinese medicine, is able to release cold, fever and asthma, mainly containing alkaloids, flavonoids, phenylpropanoids and amino acids. However, the absorption and distribution of these four category compounds in vivo still remained unclearly.

Methods

In our research, we utilized UPLC-Q-TOF-MS technique to identify the constituents within MGD, as well as the prototypes of MGD and their metabolites absorbed in plasma and brain. We further profiled the drug-time curve of prototypes and metabolites of MGD both in plasma and brain.

Results

Our results showed that 105 constituents were characterized in MGD. Thirty of them could be absorbed into blood, and ten of them could be distributed into brain. We also discovered eight new bio-transformed metabolites in blood, and a half of which could pass through the blood–brain barrier. In addition, all components detected in vivo could be absorbed and distributed immediately.

Conclusion

These findings provide an approachable method to analyze the potential bio-active compounds in MGD and their in vivo behaviors, which could promote the efficacious material basis study of MGD and the security of clinical utilization.

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来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
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