多克隆肽抗血清。

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3914-6_10

Tina H Pihl, Kristin E Engelhart, Gunnar Houen

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引用次数: 0

摘要

多克隆抗体的生产相对容易，在某些应用中可以补充单克隆抗体的不足，甚至具有某些优势。生产（多肽）抗血清的物种选择基于实际考虑，包括免疫原（疫苗）和动物的可用性。抗血清的生产受两个主要因素的制约：几天/几周内发生的适应性免疫反应的性质和动物福利的伦理准则。这里介绍了小鼠、兔子、绵羊、山羊、猪、马和鸡的简单免疫程序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Polyclonal Peptide Antisera.

Polyclonal antibodies are relatively easy to produce and may supplement monoclonal antibodies for some applications or even have some advantages.The choice of species for production of (peptide) antisera is based on practical considerations, including availability of immunogen (vaccine) and animals. Two major factors govern the production of antisera: the nature of adaptive immune responses, which take place over days/weeks and ethical guidelines for animal welfare.Here, simple procedures for immunization of mice, rabbits, sheep, goats, pigs, horses, and chickens are presented.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.