由简单 TEMPO 自由基介导的明亮发光酚电化学发光，用于可视化多重检测。

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2024-07-09 DOI:10.1016/j.talanta.2024.126530

Haidong Li, Guangyue Zhao, Yuxin Yang, Danli Zhong, Zhenxing Yang, Chengyin Wang

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引用次数: 0

摘要

本研究利用一系列具有不同官能团的 2,2,6,6-四甲基哌啶-N-氧自由基（TEMPO）作为简单催化剂，促进发光酚/H2O2 体系中电化学发光（ECL）的产生。研究发现，这些 TEMPO 自由基通过不同的催化机理促进了 H2O2 和鲁米诺的电化学氧化，以及随后鲁米诺/H2O2 体系的 ECL 生成。电化学氧化和发光酚 ECL 生成可通过 TEMPO 对位上的官能团进行调节，并揭示了其结构/活性关系。最后，结合酶系统，通过催化 4-hydroxyl-TEMPO 可使发光酚 ECL 增强 9.6 倍。增强的发光酚 ECL 可以在单电化学系统（SEES）中获得更亮的 ECL 图像，用于胆固醇、H2O2 和葡萄糖的多重检测。

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Bright luminol electrochemiluminescence mediated by a simple TEMPO radical for visualized multiplex detection

In this work, a series of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) radicals bearing different functional groups were exploited as a simple catalyst to promote electrochemiluminescence (ECL) generation in luminol/H₂O₂ system. These TEMPO radicals were found to facilitate the electrochemical oxidation of H₂O₂ and luminol through different catalytic mechanisms, as well as the subsequent ECL generation of luminol/H₂O₂ system. The electrochemical oxidation and luminol ECL generation could be tuned by the functional group on the para-position of TEMPO, for which the structure/activity relationship was revealed. Finally, with the combination of enzymatic system, luminol ECL enhancement up to 9.6-fold was obtained through the catalysis of 4-hydroxyl-TEMPO. The enhanced luminol ECL allows acquiring brighter ECL images in a single-electrochemical system (SEES) for multiplex detection of cholesterol, H₂O₂ and glucose.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.