{"title":"利用带荧光检测器的高压液相色谱法测量真菌和细菌坏死物质的精确方法，适用于无机土、有机土和泥炭土","authors":"Sylwia Adamczyk, Raisa Mäkipää, Aleksi Lehtonen, Bartosz Adamczyk","doi":"10.1016/j.pedobi.2024.150977","DOIUrl":null,"url":null,"abstract":"<div><p>Soil organic matter is the dominant pool of carbon (C) in terrestrial ecosystems. Recent advances in understanding of the mechanisms of C stabilization in the soil emphasize microbes as the main drivers. Special attention is placed on the accumulation of bacterial and fungal necromasses. This calls for development of fast and reliable methods to estimate microbial necromass in a various type of soils, including peat soils. Here we provide precise method to measure fungal and bacterial necromasses with high-pressure liquid chromatography-fluorescence detector (HPLC-FLD) and its comparison with gas chromatography method. Purity of the chromatographic peaks was confirmed with mass spectrometry. The HPLC-FLD method provides reliable results for mineral, organic and highly organic peat soils.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S003140562403498X/pdfft?md5=9138454a3c41d0432368884a8768965c&pid=1-s2.0-S003140562403498X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Precise method to measure fungal and bacterial necromass using high pressure liquid chromatography with fluorescence detector adjusted to inorganic, organic and peat soils\",\"authors\":\"Sylwia Adamczyk, Raisa Mäkipää, Aleksi Lehtonen, Bartosz Adamczyk\",\"doi\":\"10.1016/j.pedobi.2024.150977\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Soil organic matter is the dominant pool of carbon (C) in terrestrial ecosystems. Recent advances in understanding of the mechanisms of C stabilization in the soil emphasize microbes as the main drivers. Special attention is placed on the accumulation of bacterial and fungal necromasses. This calls for development of fast and reliable methods to estimate microbial necromass in a various type of soils, including peat soils. Here we provide precise method to measure fungal and bacterial necromasses with high-pressure liquid chromatography-fluorescence detector (HPLC-FLD) and its comparison with gas chromatography method. Purity of the chromatographic peaks was confirmed with mass spectrometry. The HPLC-FLD method provides reliable results for mineral, organic and highly organic peat soils.</p></div>\",\"PeriodicalId\":49711,\"journal\":{\"name\":\"Pedobiologia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-07-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S003140562403498X/pdfft?md5=9138454a3c41d0432368884a8768965c&pid=1-s2.0-S003140562403498X-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pedobiologia\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S003140562403498X\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pedobiologia","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S003140562403498X","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ECOLOGY","Score":null,"Total":0}
Precise method to measure fungal and bacterial necromass using high pressure liquid chromatography with fluorescence detector adjusted to inorganic, organic and peat soils
Soil organic matter is the dominant pool of carbon (C) in terrestrial ecosystems. Recent advances in understanding of the mechanisms of C stabilization in the soil emphasize microbes as the main drivers. Special attention is placed on the accumulation of bacterial and fungal necromasses. This calls for development of fast and reliable methods to estimate microbial necromass in a various type of soils, including peat soils. Here we provide precise method to measure fungal and bacterial necromasses with high-pressure liquid chromatography-fluorescence detector (HPLC-FLD) and its comparison with gas chromatography method. Purity of the chromatographic peaks was confirmed with mass spectrometry. The HPLC-FLD method provides reliable results for mineral, organic and highly organic peat soils.
期刊介绍：
Pedobiologia publishes peer reviewed articles describing original work in the field of soil ecology, which includes the study of soil organisms and their interactions with factors in their biotic and abiotic environments.
Analysis of biological structures, interactions, functions, and processes in soil is fundamental for understanding the dynamical nature of terrestrial ecosystems, a prerequisite for appropriate soil management. The scope of this journal consists of fundamental and applied aspects of soil ecology; key focal points include interactions among organisms in soil, organismal controls on soil processes, causes and consequences of soil biodiversity, and aboveground-belowground interactions.
We publish:
original research that tests clearly defined hypotheses addressing topics of current interest in soil ecology (including studies demonstrating nonsignificant effects);
descriptions of novel methodological approaches, or evaluations of current approaches, that address a clear need in soil ecology research;
innovative syntheses of the soil ecology literature, including metaanalyses, topical in depth reviews and short opinion/perspective pieces, and descriptions of original conceptual frameworks; and
short notes reporting novel observations of ecological significance.
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