环状 RNA 寡核苷酸：酶法合成和纳米结构支架

IF 8 2区材料科学 Q1 CHEMISTRY, PHYSICAL Nanoscale Horizons Pub Date : 2024-07-12 DOI:10.1039/D4NH00236A

Shijie Li, Yanxin Chu, Xin Guo, Chengde Mao and Shou-Jun Xiao

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引用次数: 0

摘要

我们报告了利用一种新颖的 DNA 哑铃拼接加 T4 DNA 连接策略高效合成大小范围为 16-44 nt 的单体环状 RNA（circRNA）的情况。我们中的一个研究小组最近开发出了这种 DNA 哑铃拼接策略，可将短线性 DNA 近乎定量地转化为单体环状 DNA。此外，我们还以 44 nt circRNA 为支架链，构建了混合 RNA:DNA 和纯 RNA:RNA 双交叉瓦片及其核酸纳米管和扁平阵列组装体。

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Circular RNA oligonucleotides: enzymatic synthesis and scaffolding for nanoconstruction†

We report the efficient synthesis of monomeric circular RNAs (circRNAs) in the size range of 16–44 nt with a novel DNA dumbbell splinting plus T4 DNA ligation strategy. Such a DNA dumbbell splinting strategy was developed by one group among ours recently for near-quantitative conversion of short linear DNAs into monomeric circular ones. Furthermore, using the 44 nt circRNA as scaffold strands, we constructed hybrid RNA:DNA and pure RNA:RNA double crossover tiles and their assemblies of nucleic acid nanotubes and flat arrays.

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来源期刊

Nanoscale Horizons Materials Science-General Materials Science

CiteScore

16.30

自引率

1.00%

发文量

141

期刊介绍： Nanoscale Horizons stands out as a premier journal for publishing exceptionally high-quality and innovative nanoscience and nanotechnology. The emphasis lies on original research that introduces a new concept or a novel perspective (a conceptual advance), prioritizing this over reporting technological improvements. Nevertheless, outstanding articles showcasing truly groundbreaking developments, including record-breaking performance, may also find a place in the journal. Published work must be of substantial general interest to our broad and diverse readership across the nanoscience and nanotechnology community.