使用模拟血液培养物比较 Mindray TDR 和 BacT/ALERT®3D 血液培养系统的检测时间。

IF 1.6 4区 医学 Q2 Medicine Acta Clinica Belgica Pub Date : 2024-06-01 Epub Date: 2024-07-15 DOI:10.1080/17843286.2024.2376224
Özlem Aydemir, Gökçen Ormanoğlu, Mehmet Köroğlu, Yusuf Aydemir
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引用次数: 0

摘要

目的:血液培养(BC)是诊断血流感染的标准。现有的血液培养(BC)系统都是为了缩短阳性 BC 的检测时间(TTD)而开发的。本研究旨在通过与 BacT/ALERT®3D 系统进行比较,评估 Mindray TDR 自动 BC 系统的性能:方法:使用 16 个参考菌株和 14 个临床分离菌株。方法:使用 16 个参考菌株和 14 个临床分离菌株,以 100 CFU/ml 和 10 CFU/ml 的最终浓度制备所有细菌和酵母菌菌落的序列稀释液。将配制好的溶液同时接种到两个系统的瓶中,并放入血液培养装置中:对 352 个(176 个 BacT/ALERT®3D 和 176 个 Mindray TDR-X060)血液培养瓶进行了评估,其中需氧培养瓶 336 个,厌氧培养瓶 16 个。在 10 CFU/ml 和 100 CFU/ml 稀释度下,两种系统对所有分离物的平均检测时间均无显著差异(p = 0.965,p = 0.245)。根据生物类型进行评估时,BacT/ALERT 系统对 10 CFU/ml 稀释度革兰氏阳性菌的检测时间明显较短(p = 0.019),而 Mindray 系统对酵母菌的检测时间明显较短(p = 0.047)。厌氧菌的数量太少,无法得出统计结论,但我们观察到 Mindray TDR-X060 系统的检测时间有缩短的趋势:结论:两种操作原理相似的系统在阳性检测时间方面表现出不同的浓度依赖性,这取决于微生物的类型。在高浓度下使用 Mindray TDR-X060 系统是安全的,而在低浓度下则需要对新引进的 Mindray 系统进行进一步的比较研究。
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Comparison of time-to-detection of Mindray TDR and BacT/ALERT®3D blood culture systems using simulated blood cultures.

Purpose: Blood culture (BC) is the standard for diagnosing bloodstream infections. Available blood culture (BC) systems have been developed to shorten the time to detection (TTD) of positive BCs. This study aimed to evaluate the performance of the Mindray TDR automatic BC system by comparing it with the BacT/ALERT®3D system.

Methods: Sixteen reference strains and 14 clinical isolates were used. Serial dilutions were prepared from all bacterial and yeast colonies with a final concentration of 100 CFU/ml and 10 CFU/ml. The prepared solutions were simultaneously inoculated into the bottles of both systems and placed in blood culture devices.

Results: Three hundred and fifty-two (176 BacT/ALERT®3D and 176 Mindray TDR-X060) blood culture bottles were evaluated, 336 aerobic and 16 anaerobic. At both 10 CFU/ml and 100 CFU/ml dilution, there was no significant difference between the two systems in terms of mean detection times for all isolates (p = 0.965, p = 0.245). When evaluated according to the type of organism, the detection time of gram-positive bacteria at 10 CFU/ml dilution was significantly shorter in the BacT/ALERT system (p = 0.019), whereas detection time for yeasts was significantly shorter with the Mindray system (p = 0.047). The number of anaerobic bacteria was too small to draw statistical conclusions, but we observed a trend of shorter detection times in the Mindray TDR-X060 system.

Conclusion: Two systems with similar operating principles showed different concentrations-dependent performances in terms of positivity detection times depending on the type of microorganism. Mindray TDR-X060 system has been found to be safe to use at high concentrations with this at lower concentrations further comparative studies are needed on the newly introduced Mindray system.

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来源期刊
Acta Clinica Belgica
Acta Clinica Belgica 医学-医学:内科
CiteScore
2.90
自引率
0.00%
发文量
44
审稿时长
6-12 weeks
期刊介绍: Acta Clinica Belgica: International Journal of Clinical and Laboratory Medicine primarily publishes papers on clinical medicine, clinical chemistry, pathology and molecular biology, provided they describe results which contribute to our understanding of clinical problems or describe new methods applicable to clinical investigation. Readership includes physicians, pathologists, pharmacists and physicians working in non-academic and academic hospitals, practicing internal medicine and its subspecialties.
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