CRISPR 在寄生虫学中的应用。

IF 2.2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Current pharmaceutical biotechnology Pub Date : 2024-07-12 DOI:10.2174/0113892010316710240626042205

Phoebe Yon Ern Tee, Sylvester Yee Chun Chu, Chloe Chien Yee Kok, Mun Foo, Clarence Zhen Jin Tan, Jhi Biau Foo, Fazleen Haslinda Mohd Hatta, Li Kar Stella Tan, Sharina Hamzah

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引用次数: 0

摘要

基于基因组互套密码重复序列（CRISPR）的技术已被用于农业、生物技术和医学等多个研究领域。通过使用短序列向导 RNA 和 CRISPR 相关（Cas）蛋白，这种技术可以对基因组进行稳健、特定位点的操作，帮助研究人员取得重要的生物医学发现和科学进步。在这篇综述中，我们探讨了 CRISPR/Cas 系统在寄生虫学领域的应用，包括新型功能基因的鉴定和验证、寄生虫感染的诊断、降低寄生虫的毒性以及破坏疾病的传播。我们还讨论了如何将 CRISPR 用于开发治疗药物、疫苗和药物发现。此外，我们还强调了这项技术面临的挑战和未来前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Applications of CRISPR in Parasitology.

Clustered Regions of Interspersed Palindromic Repeat (CRISPR)-based techniques have been utilized in various research areas, including agriculture, biotechnology, and medicine. With the use of a short sequence guide RNA and CRISPR-associated (Cas) protein, this technique allows for robust, site-specific manipulation of the genome, aiding researchers in making important biomedical discoveries and scientific advancements. In this review, we explored the applications of CRISPR/Cas systems in the field of parasitology for the identification and validation of novel functional genes, diagnosis of parasitic infections, reduction of parasite virulence, and the disruption of disease transmission. We also discussed how CRISPR can be used for the development of therapeutics, vaccines, and drug discovery. Furthermore, the challenges and future perspectives of this technology are also highlighted.

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来源期刊

Current pharmaceutical biotechnology 医学-生化与分子生物学

CiteScore

5.60

自引率

3.60%

发文量

203

审稿时长

6 months

期刊介绍： Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal includes timely in-depth reviews, original research articles and letters written by leaders in the field, covering a range of current topics in scientific areas of Pharmaceutical Biotechnology. Invited and unsolicited review articles are welcome. The journal encourages contributions describing research at the interface of drug discovery and pharmacological applications, involving in vitro investigations and pre-clinical or clinical studies. Scientific areas within the scope of the journal include pharmaceutical chemistry, biochemistry and genetics, molecular and cellular biology, and polymer and materials sciences as they relate to pharmaceutical science and biotechnology. In addition, the journal also considers comprehensive studies and research advances pertaining food chemistry with pharmaceutical implication. Areas of interest include: DNA/protein engineering and processing Synthetic biotechnology Omics (genomics, proteomics, metabolomics and systems biology) Therapeutic biotechnology (gene therapy, peptide inhibitors, enzymes) Drug delivery and targeting Nanobiotechnology Molecular pharmaceutics and molecular pharmacology Analytical biotechnology (biosensing, advanced technology for detection of bioanalytes) Pharmacokinetics and pharmacodynamics Applied Microbiology Bioinformatics (computational biopharmaceutics and modeling) Environmental biotechnology Regenerative medicine (stem cells, tissue engineering and biomaterials) Translational immunology (cell therapies, antibody engineering, xenotransplantation) Industrial bioprocesses for drug production and development Biosafety Biotech ethics Special Issues devoted to crucial topics, providing the latest comprehensive information on cutting-edge areas of research and technological advances, are welcome. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.