Hossein Rezvan, Selman A Ali, Sahar Hamoonnavard, Robert Rees
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引用次数: 0
摘要
肿瘤坏死因子受体 OX40（CD134）是刺激 T 淋巴细胞功能的重要分子之一，它被其同源配体 OX40L（CD134L，CD252）激活。OX40L 的相互作用被认为是治疗感染性和非感染性疾病的潜在治疗靶点。本研究的主要目的是测定 MBI 和 MEP 这两种新型树脂纯化 OX40L-IgG 融合蛋白的效力以及这种 OX40L-IgG 融合蛋白的生物活性。将这些树脂纯化的 OX40L-IgG 的生物活性与蛋白 A sepharose 树脂进行比较。与注射 PBS 的对照组相比，用三种树脂纯化的相同剂量的 OX40L 处理小鼠后，肿瘤生长明显延迟。与注射其他树脂纯化的 OX40L 的小鼠相比，注射 MBI 树脂纯化的 OX40L 的小鼠的肿瘤细胞（CT26）生长明显延迟。
Biological Activity of Mouse OX40L-Igg Purified With Two Novel Resins.
One of the important stimulating molecules for the function of T lymphocytes is tumor necrosis factor receptor OX40 (CD134), activated by its cognate ligand OX40L (CD134L, CD252). OX40L interactions have been proposed as a potential therapeutic target for treating infectious and non-infectious diseases. The main purpose of this study was to determine the potency of two novel resins MBI and MEP for the purification of OX40L-IgG fusion protein and the biological activities of this OX40L-IgG fusion protein. The biological activity of the OX40L-IgG purified by these resins compared with protein A sepharose resin. Mice treated with the same doses of the OX40L purified by the three resins showed a significant delay in tumor growth compared to the controls injected with PBS. Mice treated with the OX40L purified by MBI resin showed a significant delay in tumor cell (CT26) growth compared with mice injected with OX40L purified by other resins.
期刊介绍：
The International Journal of Molecular and Cellular Medicine (IJMCM) is a peer-reviewed, quarterly publication of Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. The journal covers all cellular & molecular biology and medicine disciplines such as the genetic basis of disease, biomarker discovery in diagnosis and treatment, genomics and proteomics, bioinformatics, computer applications in human biology, stem cells and tissue engineering, medical biotechnology, nanomedicine, cellular processes related to growth, death and survival, clinical biochemistry, molecular & cellular immunology, molecular and cellular aspects of infectious disease and cancer research. IJMCM is a free access journal. All open access articles published in IJMCM are distributed under the terms of the Creative Commons Attribution CC BY. The journal doesn''t have any submission and article processing charges (APCs).
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